Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies.

The maintenance of adult stem cells in their normal quiescent state depends on intrinsic factors and extrinsic signals originating from their microenvironment (also known as the stem cell niche). In skeletal muscle, its stem cells (satellite cells) lose their regenerative potential with aging, and t...

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Detalles Bibliográficos
Autores: Perdiguero, Eusebio, Moiseeva, Victoria, Munoz-Canoves, Pura
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/10606
Acceso en línea:http://hdl.handle.net/20.500.12105/10606
Access Level:acceso abierto
Palabra clave:Adult Stem Cells
Aging
Animals
Antibodies
Endothelial Cells
Flow Cytometry
Macrophages
Mesenchymal Stem Cells
Mice
Muscle, Skeletal
Real-Time Polymerase Chain Reaction
Regeneration
Satellite Cells, Skeletal Muscle
Stem Cell Niche
Workflow
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network_acronym_str ES
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repository_id_str
spelling Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies.Perdiguero, EusebioMoiseeva, VictoriaMunoz-Canoves, PuraAdult Stem CellsAgingAnimalsAntibodiesEndothelial CellsFlow CytometryMacrophagesMesenchymal Stem CellsMiceMuscle, SkeletalReal-Time Polymerase Chain ReactionRegenerationSatellite Cells, Skeletal MuscleStem Cell NicheWorkflowThe maintenance of adult stem cells in their normal quiescent state depends on intrinsic factors and extrinsic signals originating from their microenvironment (also known as the stem cell niche). In skeletal muscle, its stem cells (satellite cells) lose their regenerative potential with aging, and this has been attributed, at least in part, to both age-associated changes in the satellite cells as in the niche cells, which include resident fibro-adipogenic progenitors (FAPs), macrophages, and endothelial cells, among others. To understand the regenerative decline of skeletal muscle with aging, there is a need for methods to specifically isolate stem and niche cells from resting muscle. Here we describe a fluorescence-activated cell sorting (FACS) protocol to simultaneously isolate discrete populations of satellite cells and niche cells from skeletal muscle of aging mice.Humana PressMinisterio de Ciencia, Innovación y Universidades (España)Unión Europea. Comisión Europea. European Research Council (ERC)Fundación La Caixa20202020-06-2920192019-02-0120192019-02-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/10606reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)InglésengES SEV-2015-0505 Not availableES SAF2015-67369-R Not availableES SAF2015-70270-REDT Not availableES MDM-2014-0370 Not availableEuropean Commission http://dx.doi.org/10.13039/501100000780 Horizon 2020 Framework Programme 741966open accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/106062026-06-12T12:43:37Z
dc.title.none.fl_str_mv Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies.
title Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies.
spellingShingle Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies.
Perdiguero, Eusebio
Adult Stem Cells
Aging
Animals
Antibodies
Endothelial Cells
Flow Cytometry
Macrophages
Mesenchymal Stem Cells
Mice
Muscle, Skeletal
Real-Time Polymerase Chain Reaction
Regeneration
Satellite Cells, Skeletal Muscle
Stem Cell Niche
Workflow
title_short Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies.
title_full Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies.
title_fullStr Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies.
title_full_unstemmed Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies.
title_sort Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies.
dc.creator.none.fl_str_mv Perdiguero, Eusebio
Moiseeva, Victoria
Munoz-Canoves, Pura
author Perdiguero, Eusebio
author_facet Perdiguero, Eusebio
Moiseeva, Victoria
Munoz-Canoves, Pura
author_role author
author2 Moiseeva, Victoria
Munoz-Canoves, Pura
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Unión Europea. Comisión Europea. European Research Council (ERC)
Fundación La Caixa
dc.subject.none.fl_str_mv Adult Stem Cells
Aging
Animals
Antibodies
Endothelial Cells
Flow Cytometry
Macrophages
Mesenchymal Stem Cells
Mice
Muscle, Skeletal
Real-Time Polymerase Chain Reaction
Regeneration
Satellite Cells, Skeletal Muscle
Stem Cell Niche
Workflow
topic Adult Stem Cells
Aging
Animals
Antibodies
Endothelial Cells
Flow Cytometry
Macrophages
Mesenchymal Stem Cells
Mice
Muscle, Skeletal
Real-Time Polymerase Chain Reaction
Regeneration
Satellite Cells, Skeletal Muscle
Stem Cell Niche
Workflow
description The maintenance of adult stem cells in their normal quiescent state depends on intrinsic factors and extrinsic signals originating from their microenvironment (also known as the stem cell niche). In skeletal muscle, its stem cells (satellite cells) lose their regenerative potential with aging, and this has been attributed, at least in part, to both age-associated changes in the satellite cells as in the niche cells, which include resident fibro-adipogenic progenitors (FAPs), macrophages, and endothelial cells, among others. To understand the regenerative decline of skeletal muscle with aging, there is a need for methods to specifically isolate stem and niche cells from resting muscle. Here we describe a fluorescence-activated cell sorting (FACS) protocol to simultaneously isolate discrete populations of satellite cells and niche cells from skeletal muscle of aging mice.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-02-01
2019
2019-02-01
2020
2020-06-29
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/10606
url http://hdl.handle.net/20.500.12105/10606
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv ES SEV-2015-0505 Not available
ES SAF2015-67369-R Not available
ES SAF2015-70270-REDT Not available
ES MDM-2014-0370 Not available
European Commission http://dx.doi.org/10.13039/501100000780 Horizon 2020 Framework Programme 741966
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Humana Press
publisher.none.fl_str_mv Humana Press
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869424728601526272
score 15,812429

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