Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies.
The maintenance of adult stem cells in their normal quiescent state depends on intrinsic factors and extrinsic signals originating from their microenvironment (also known as the stem cell niche). In skeletal muscle, its stem cells (satellite cells) lose their regenerative potential with aging, and t...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | Instituto de Salud Carlos III (ISCIII) |
| Repositorio: | Repisalud |
| Idioma: | inglés |
| OAI Identifier: | oai:repisalud.isciii.es:20.500.12105/10606 |
| Acceso en línea: | http://hdl.handle.net/20.500.12105/10606 |
| Access Level: | acceso abierto |
| Palabra clave: | Adult Stem Cells Aging Animals Antibodies Endothelial Cells Flow Cytometry Macrophages Mesenchymal Stem Cells Mice Muscle, Skeletal Real-Time Polymerase Chain Reaction Regeneration Satellite Cells, Skeletal Muscle Stem Cell Niche Workflow |
| Sumario: | The maintenance of adult stem cells in their normal quiescent state depends on intrinsic factors and extrinsic signals originating from their microenvironment (also known as the stem cell niche). In skeletal muscle, its stem cells (satellite cells) lose their regenerative potential with aging, and this has been attributed, at least in part, to both age-associated changes in the satellite cells as in the niche cells, which include resident fibro-adipogenic progenitors (FAPs), macrophages, and endothelial cells, among others. To understand the regenerative decline of skeletal muscle with aging, there is a need for methods to specifically isolate stem and niche cells from resting muscle. Here we describe a fluorescence-activated cell sorting (FACS) protocol to simultaneously isolate discrete populations of satellite cells and niche cells from skeletal muscle of aging mice. |
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