RNA-sequencing reveals genes linked with oocyte developmental potential in bovine cumulus cells

Cumulus cells provide an interesting biological material to perform analyses to understand the molecular clues determining oocyte competence. The objective of this study was to analyze the transcriptional differences between cumulus cells from oocytes exhibiting different developmental potentials fo...

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Autores: Martínez-Moro, Álvaro, González-Brusi, Leopoldo, Lamas-Toranzo, Ismael, O'Callaghan, Elena, Esteve-Codina, Anna, Lonergan, Pat, Bermejo-Álvarez, Pablo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/54667
Acceso en línea:http://hdl.handle.net/10230/54667
http://dx.doi.org/10.1002/mrd.23631
Access Level:acceso abierto
Palabra clave:Granulosa cell
In vitro maturation
Integrin
Oocyte quality
Transcription
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spelling RNA-sequencing reveals genes linked with oocyte developmental potential in bovine cumulus cellsMartínez-Moro, ÁlvaroGonzález-Brusi, LeopoldoLamas-Toranzo, IsmaelO'Callaghan, ElenaEsteve-Codina, AnnaLonergan, PatBermejo-Álvarez, PabloGranulosa cellIn vitro maturationIntegrinOocyte qualityTranscriptionCumulus cells provide an interesting biological material to perform analyses to understand the molecular clues determining oocyte competence. The objective of this study was to analyze the transcriptional differences between cumulus cells from oocytes exhibiting different developmental potentials following individual in vitro embryo production by RNA-seq. Cumulus cells were allocated into three groups according to the developmental potential of the oocyte following fertilization: (1) oocytes developing to blastocysts (Bl+), (2) oocytes cleaving but arresting development before the blastocyst stage (Bl-), and (3) oocytes not cleaving (Cl-). RNAseq was performed on 4 (Cl-) or 5 samples (Bl+ and Bl-) of cumulus cells pooled from 10 cumulus-oocyte complexes per group. A total of 49, 50, and 18 differentially expressed genes (DEGs) were detected in the comparisons Bl+ versus Bl-, Bl+ versus Cl- and Bl- versus Cl-, respectively, showing a fold change greater than 1.5 at an adjusted p value <0.05. Focussing on DEGs in cumulus cells from Bl+ group, 10 DEGs were common to both comparisons (10/49 from Bl+ vs. Bl-, 10/50 from Bl+ vs. Cl-). These DEGs correspond to 6 upregulated genes (HBE1, ITGA1, PAPPA, AKAP12, ITGA5, and SLC1A4), and 4 downregulated genes (GSTA1, PSMB8, FMOD, and SFRP4) in Bl+ compared to the other groups, from which 7 were validated by quantitative PCR (HBE1, ITGA1, PAPPA, AKAP12, ITGA5, PSMB8 and SFRP4). These genes are involved in critical biological functions such as integrin-mediated cell adhesion, oxygen availability, IGF and Wnt signaling or PKA pathway, highlighting specific biological processes altered in incompetent in vitro maturation oocytes.This work was funded by the projects IND2017/BIO-7748 from Madrid Region Government and AGL2017-84908-R and PID2020-117501RB-I00 by the Spanish Ministry of Economy, Industry and Competitiveness (MINECO). AMM was funded by project IND2017/BIO-7748 and ILT by a FPI fellowship by MINECO.Wiley202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/54667http://dx.doi.org/10.1002/mrd.23631reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésMol Reprod Dev. 2022 Sep;89(9):399-412info:eu-repo/grantAgreement/ES/2PE/AGL2017-84908-Rinfo:eu-repo/grantAgreement/ES/2PE/PID2020-117501RB-I00© 2022 The Authors. Molecular Reproduction and Development published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/546672026-06-12T07:21:37Z
dc.title.none.fl_str_mv RNA-sequencing reveals genes linked with oocyte developmental potential in bovine cumulus cells
title RNA-sequencing reveals genes linked with oocyte developmental potential in bovine cumulus cells
spellingShingle RNA-sequencing reveals genes linked with oocyte developmental potential in bovine cumulus cells
Martínez-Moro, Álvaro
Granulosa cell
In vitro maturation
Integrin
Oocyte quality
Transcription
title_short RNA-sequencing reveals genes linked with oocyte developmental potential in bovine cumulus cells
title_full RNA-sequencing reveals genes linked with oocyte developmental potential in bovine cumulus cells
title_fullStr RNA-sequencing reveals genes linked with oocyte developmental potential in bovine cumulus cells
title_full_unstemmed RNA-sequencing reveals genes linked with oocyte developmental potential in bovine cumulus cells
title_sort RNA-sequencing reveals genes linked with oocyte developmental potential in bovine cumulus cells
dc.creator.none.fl_str_mv Martínez-Moro, Álvaro
González-Brusi, Leopoldo
Lamas-Toranzo, Ismael
O'Callaghan, Elena
Esteve-Codina, Anna
Lonergan, Pat
Bermejo-Álvarez, Pablo
author Martínez-Moro, Álvaro
author_facet Martínez-Moro, Álvaro
González-Brusi, Leopoldo
Lamas-Toranzo, Ismael
O'Callaghan, Elena
Esteve-Codina, Anna
Lonergan, Pat
Bermejo-Álvarez, Pablo
author_role author
author2 González-Brusi, Leopoldo
Lamas-Toranzo, Ismael
O'Callaghan, Elena
Esteve-Codina, Anna
Lonergan, Pat
Bermejo-Álvarez, Pablo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Granulosa cell
In vitro maturation
Integrin
Oocyte quality
Transcription
topic Granulosa cell
In vitro maturation
Integrin
Oocyte quality
Transcription
description Cumulus cells provide an interesting biological material to perform analyses to understand the molecular clues determining oocyte competence. The objective of this study was to analyze the transcriptional differences between cumulus cells from oocytes exhibiting different developmental potentials following individual in vitro embryo production by RNA-seq. Cumulus cells were allocated into three groups according to the developmental potential of the oocyte following fertilization: (1) oocytes developing to blastocysts (Bl+), (2) oocytes cleaving but arresting development before the blastocyst stage (Bl-), and (3) oocytes not cleaving (Cl-). RNAseq was performed on 4 (Cl-) or 5 samples (Bl+ and Bl-) of cumulus cells pooled from 10 cumulus-oocyte complexes per group. A total of 49, 50, and 18 differentially expressed genes (DEGs) were detected in the comparisons Bl+ versus Bl-, Bl+ versus Cl- and Bl- versus Cl-, respectively, showing a fold change greater than 1.5 at an adjusted p value <0.05. Focussing on DEGs in cumulus cells from Bl+ group, 10 DEGs were common to both comparisons (10/49 from Bl+ vs. Bl-, 10/50 from Bl+ vs. Cl-). These DEGs correspond to 6 upregulated genes (HBE1, ITGA1, PAPPA, AKAP12, ITGA5, and SLC1A4), and 4 downregulated genes (GSTA1, PSMB8, FMOD, and SFRP4) in Bl+ compared to the other groups, from which 7 were validated by quantitative PCR (HBE1, ITGA1, PAPPA, AKAP12, ITGA5, PSMB8 and SFRP4). These genes are involved in critical biological functions such as integrin-mediated cell adhesion, oxygen availability, IGF and Wnt signaling or PKA pathway, highlighting specific biological processes altered in incompetent in vitro maturation oocytes.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/54667
http://dx.doi.org/10.1002/mrd.23631
url http://hdl.handle.net/10230/54667
http://dx.doi.org/10.1002/mrd.23631
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Mol Reprod Dev. 2022 Sep;89(9):399-412
info:eu-repo/grantAgreement/ES/2PE/AGL2017-84908-R
info:eu-repo/grantAgreement/ES/2PE/PID2020-117501RB-I00
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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