In vitro production of bovine embryos: Cumulus/granulosa cell gene expression patterns point to early atresia as beneficial for oocyte competence

In vitro production (IVP) of bovine embryos has become widespread technology implemented in cattle breeding and production. Here, we review novel data on cumulus/granulosa cell gene expression, as determined by RNAseq on cellular material from pooled follicular fluids at the single animal level, and...

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Detalles Bibliográficos
Autores: Mazzoni, Gianluca, Razza, Eduardo, Pedersen, Hanne S., Secher, Jan, Kadarmideen, Haja N., Callesen, Henrik, Stroebech, Lotte, Freude, Kristine, Hyttel, Poul
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/231415
Acceso en línea:http://dx.doi.org/10.21451/1984-3143-AR990
http://hdl.handle.net/11449/231415
Access Level:acceso abierto
Palabra clave:Atresia
Biomarkers
Cattle
Granulosa cells
IVP
Oocyte competence
Transcriptomics
Descripción
Sumario:In vitro production (IVP) of bovine embryos has become widespread technology implemented in cattle breeding and production. Here, we review novel data on cumulus/granulosa cell gene expression, as determined by RNAseq on cellular material from pooled follicular fluids at the single animal level, and relate these finding to previous data on oocyte developmental competence and ultrastructure. The cumulus/granulosa cell gene expression patterns indicate that early follicular atresia is associated with increased blastocyst yield and this hypothesis is supported by previous data on oocyte competence and ultrastructure.