Characterization and partial sequencing of species-specific sarcoplasmic polypeptides from commercial hake species by mass spectrometry following two-dimensional electrophoresis

8 páginas, 1 tabla, 4 figuras

Detalhes bibliográficos
Autores: Piñeiro, Carmen, Vázquez, Jesús, Marina, Anabel, Barros-Velázquez, Jorge, Gallardo, José Manuel
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2001
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/56696
Acesso em linha:http://hdl.handle.net/10261/56696
Access Level:acceso abierto
Palavra-chave:Proteomics
Two-dimensional electrophoresis
Mass spectrometry
Polypeptide
Characterization
Hake
Parvalbumin
Species identification
Nucleoside diphosphate kinase
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spelling Characterization and partial sequencing of species-specific sarcoplasmic polypeptides from commercial hake species by mass spectrometry following two-dimensional electrophoresisPiñeiro, CarmenVázquez, JesúsMarina, AnabelBarros-Velázquez, JorgeGallardo, José ManuelProteomicsTwo-dimensional electrophoresisMass spectrometryPolypeptideCharacterizationHakeParvalbuminSpecies identificationNucleoside diphosphate kinase8 páginas, 1 tabla, 4 figurasThe Merluccidae family comprises marine species, some of them of high commercial value and others less appreciated, whose commercialization in Europe under the generic name of “hake” is highly remarkable. The potential of proteomics was employed in this study with the aim of achieving the differential characterization of five different hake species: Merluccius merluccius (European hake), M. australis (Southern hake), M. hubbsi (Argentinian hake), M. gayi (Chilean hake), and M. capensis (Cape hake), some of them very closely related. Species-specific polypeptides were observed for the five hake species studied in isoelectric focusing (IEF) and/or two-dimensional electrophoresis (2-DE) high-resolution gels. The peptide mass maps of two polypeptide groups, previously selected by 2-DE analysis as potentially species-specific, were obtained by “in-gel” tryptic digestion, followed by matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS). Analysis of group A polypeptides (with pI in the range of 5.0–5.5 and molecular mass of 17 kDa), allowed the differential classification of the hake species into two groups: the East Atlantic coast group and the West Atlantic coast group. Moreover, the peptide mass-maps from the heat-resistant parvalbumin fraction (pI below 4.5; molecular mass <12 kDa) allowed the detection of a peptide characteristic of M. australis not present in the other four hake species tested. A specific 17 kDa protein from M. merluccius was also partially sequenced by nanospray-ion trap-tandem MS, revealing a high homology with rat nucleoside diphosphate kinase A (NDKA). This work opens the way to the application of proteomics to the differential characterization of commercial hake species at the molecular levelThis work was partially supported by the Comision Interministerial de Ciencia y Tecnologia (CICYT) (Project ALI95-0053)Peer reviewedWiley-BlackwellComisión Interministerial de Ciencia y Tecnología, CICYT (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201220122001info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/56696reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1002/1522-2683(200105)22:8<1545::AID-ELPS1545>3.0.CO;2-5Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/566962026-05-22T06:33:51Z
dc.title.none.fl_str_mv Characterization and partial sequencing of species-specific sarcoplasmic polypeptides from commercial hake species by mass spectrometry following two-dimensional electrophoresis
title Characterization and partial sequencing of species-specific sarcoplasmic polypeptides from commercial hake species by mass spectrometry following two-dimensional electrophoresis
spellingShingle Characterization and partial sequencing of species-specific sarcoplasmic polypeptides from commercial hake species by mass spectrometry following two-dimensional electrophoresis
Piñeiro, Carmen
Proteomics
Two-dimensional electrophoresis
Mass spectrometry
Polypeptide
Characterization
Hake
Parvalbumin
Species identification
Nucleoside diphosphate kinase
title_short Characterization and partial sequencing of species-specific sarcoplasmic polypeptides from commercial hake species by mass spectrometry following two-dimensional electrophoresis
title_full Characterization and partial sequencing of species-specific sarcoplasmic polypeptides from commercial hake species by mass spectrometry following two-dimensional electrophoresis
title_fullStr Characterization and partial sequencing of species-specific sarcoplasmic polypeptides from commercial hake species by mass spectrometry following two-dimensional electrophoresis
title_full_unstemmed Characterization and partial sequencing of species-specific sarcoplasmic polypeptides from commercial hake species by mass spectrometry following two-dimensional electrophoresis
title_sort Characterization and partial sequencing of species-specific sarcoplasmic polypeptides from commercial hake species by mass spectrometry following two-dimensional electrophoresis
dc.creator.none.fl_str_mv Piñeiro, Carmen
Vázquez, Jesús
Marina, Anabel
Barros-Velázquez, Jorge
Gallardo, José Manuel
author Piñeiro, Carmen
author_facet Piñeiro, Carmen
Vázquez, Jesús
Marina, Anabel
Barros-Velázquez, Jorge
Gallardo, José Manuel
author_role author
author2 Vázquez, Jesús
Marina, Anabel
Barros-Velázquez, Jorge
Gallardo, José Manuel
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Proteomics
Two-dimensional electrophoresis
Mass spectrometry
Polypeptide
Characterization
Hake
Parvalbumin
Species identification
Nucleoside diphosphate kinase
topic Proteomics
Two-dimensional electrophoresis
Mass spectrometry
Polypeptide
Characterization
Hake
Parvalbumin
Species identification
Nucleoside diphosphate kinase
description 8 páginas, 1 tabla, 4 figuras
publishDate 2001
dc.date.none.fl_str_mv 2001
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/56696
url http://hdl.handle.net/10261/56696
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1002/1522-2683(200105)22:8<1545::AID-ELPS1545>3.0.CO;2-5
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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