INO80 complex regulates the G1-to-S transcriptional wave through MBF
In eukaryotic cells a key regulatory step of cell cycle occurs in late G1, which has been termed “Start” in yeast cells. At this point, cells determine whether they will go to through a new round of proliferation, or choose alternative pathways; cell cycle arrest or sexual differentiation. In fissio...
| Author: | |
|---|---|
| Format: | doctoral thesis |
| Status: | Published version |
| Publication Date: | 2015 |
| Country: | España |
| Institution: | CBUC, CESCA |
| Repository: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/586378 |
| Online Access: | http://hdl.handle.net/10803/586378 |
| Access Level: | Open access |
| Keyword: | S. pombe MBF INO80 Transcription factor DNA damage Les4 Histona H2A.Z Nucleosomas Transcripción Ciclo celular 576 |
| Summary: | In eukaryotic cells a key regulatory step of cell cycle occurs in late G1, which has been termed “Start” in yeast cells. At this point, cells determine whether they will go to through a new round of proliferation, or choose alternative pathways; cell cycle arrest or sexual differentiation. In fission yeast, the MBF complex controls the transcriptional activation of some genes of G1/S transcriptional wave, including cdc18, cdt1 (necessary to prevent onset of mitosis before of completion of DNA synthesis), and cdc22 (ribonucleotide reductase). Control of MBF activity is essential for normal cell cycle progression. It has been found that MBF complex is bound to the promoter of its target genes throughout the cell cycle, implicating that MBF activity is not regulated by pure binding to DNA. We purified novel inetractor of MBF complex, INO80 complex. Here we demonstrate that INO80 regulates cell cycle genes through MBF, and that proper acetylation of histone variant H2A.Z is crucial for MBF dependent transcription. |
|---|