Biodegradation of carbon materials by environmental peroxidases depends on the type of allotropic form

Carbon nanomaterials, possessing unique properties and advantages, exhibit broad application prospects. However, their potential risks to life and the environment have constrained their development. Investigating various degradation strategies can mitigate their adverse effects and expand their appl...

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Autores: Wang, Tengfei, Dasgupta, Nandita, Artiga, Álvaro, Janica, Iwona, Tamayo Ramos, Juan Antonio, Rumbo, Carlos, Bianco, Alberto
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/401518
Acceso en línea:http://hdl.handle.net/10261/401518
https://api.elsevier.com/content/abstract/scopus_id/105015149786
Access Level:acceso abierto
Palabra clave:Graphene
Horseradish peroxidase
Manganese peroxidase
Pichia pastoris
Raman
Single-wall carbon nanotubes
graphene
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spelling Biodegradation of carbon materials by environmental peroxidases depends on the type of allotropic formWang, TengfeiDasgupta, NanditaArtiga, ÁlvaroJanica, IwonaTamayo Ramos, Juan AntonioRumbo, CarlosBianco, AlbertoGrapheneHorseradish peroxidaseManganese peroxidasePichia pastorisRamanSingle-wall carbon nanotubesPichia pastorisgrapheneCarbon nanomaterials, possessing unique properties and advantages, exhibit broad application prospects. However, their potential risks to life and the environment have constrained their development. Investigating various degradation strategies can mitigate their adverse effects and expand their applications, particularly within the fields of life and materials sciences. Peroxidases are widely utilized for degradation due to their capability to catalyse the breakdown of various organic compounds. In this study, three peroxidases, namely horseradish peroxidase (HRP), Pichia pastoris-expressed Eucodis® peroxidase (EP 13), and manganese peroxidase (MnP), were selected to investigate their effects on the enzymatic biodegradation of different allotropic forms of carbon materials, including graphene and single-wall carbon nanotubes (SWCNT). The obvious increase of defects and decomposition of the structures were demonstrated for graphene by Raman spectroscopy and transmission electron microscope (TEM) after the treatment with these peroxidases. No degradation was instead observed in the enzyme-treated pristine SWCNT. The differences of degradation in two carbon nanomaterials are supposed to result from their distinct physicochemical properties. X-ray photoelectron spectroscopy (XPS) and thermogravimetric analysis (TGA) evidenced that a number of oxygen-containing functional groups are present in graphene, likely providing the catalytic sites for the peroxidase action thus facilitating its degradation, as previously demonstrated using other types of oxidative conditions.This work is supported by funding from the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 953152 (DIAGONAL). The authors would like to thank Simone Ligi from Graphene-XT, and Gunther Van Kerckhove from OCSiAl Europe Sarl from providing the materials and critically reading the manuscript, Cathy Royer from Plateforme Imagerie In Vitro de l’ITI Neurostra (CNRS UAR 3156, University of Strasbourg) for the sample fixation and TEM observations. N. D. would like to acknowledge the financial support received from Maria Zambrano aid modality financed by Next Generation EU.Peer reviewedElsevierEuropean CommissionArtiga, Álvaro [0000-0001-9475-6367]Rumbo, Carlos [0000-0002-5038-0334]Bianco, Alberto [0000-0002-1090-296X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/401518https://api.elsevier.com/content/abstract/scopus_id/105015149786reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/953152Journal of Environmental Chemical EngineeringThe underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1016/j.jece.2025.118671https://doi.org/10.1016/j.jece.2025.118671Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4015182026-05-22T06:33:51Z
dc.title.none.fl_str_mv Biodegradation of carbon materials by environmental peroxidases depends on the type of allotropic form
title Biodegradation of carbon materials by environmental peroxidases depends on the type of allotropic form
spellingShingle Biodegradation of carbon materials by environmental peroxidases depends on the type of allotropic form
Wang, Tengfei
Graphene
Horseradish peroxidase
Manganese peroxidase
Pichia pastoris
Raman
Single-wall carbon nanotubes
Pichia pastoris
graphene
title_short Biodegradation of carbon materials by environmental peroxidases depends on the type of allotropic form
title_full Biodegradation of carbon materials by environmental peroxidases depends on the type of allotropic form
title_fullStr Biodegradation of carbon materials by environmental peroxidases depends on the type of allotropic form
title_full_unstemmed Biodegradation of carbon materials by environmental peroxidases depends on the type of allotropic form
title_sort Biodegradation of carbon materials by environmental peroxidases depends on the type of allotropic form
dc.creator.none.fl_str_mv Wang, Tengfei
Dasgupta, Nandita
Artiga, Álvaro
Janica, Iwona
Tamayo Ramos, Juan Antonio
Rumbo, Carlos
Bianco, Alberto
author Wang, Tengfei
author_facet Wang, Tengfei
Dasgupta, Nandita
Artiga, Álvaro
Janica, Iwona
Tamayo Ramos, Juan Antonio
Rumbo, Carlos
Bianco, Alberto
author_role author
author2 Dasgupta, Nandita
Artiga, Álvaro
Janica, Iwona
Tamayo Ramos, Juan Antonio
Rumbo, Carlos
Bianco, Alberto
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Artiga, Álvaro [0000-0001-9475-6367]
Rumbo, Carlos [0000-0002-5038-0334]
Bianco, Alberto [0000-0002-1090-296X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Graphene
Horseradish peroxidase
Manganese peroxidase
Pichia pastoris
Raman
Single-wall carbon nanotubes
Pichia pastoris
graphene
topic Graphene
Horseradish peroxidase
Manganese peroxidase
Pichia pastoris
Raman
Single-wall carbon nanotubes
Pichia pastoris
graphene
description Carbon nanomaterials, possessing unique properties and advantages, exhibit broad application prospects. However, their potential risks to life and the environment have constrained their development. Investigating various degradation strategies can mitigate their adverse effects and expand their applications, particularly within the fields of life and materials sciences. Peroxidases are widely utilized for degradation due to their capability to catalyse the breakdown of various organic compounds. In this study, three peroxidases, namely horseradish peroxidase (HRP), Pichia pastoris-expressed Eucodis® peroxidase (EP 13), and manganese peroxidase (MnP), were selected to investigate their effects on the enzymatic biodegradation of different allotropic forms of carbon materials, including graphene and single-wall carbon nanotubes (SWCNT). The obvious increase of defects and decomposition of the structures were demonstrated for graphene by Raman spectroscopy and transmission electron microscope (TEM) after the treatment with these peroxidases. No degradation was instead observed in the enzyme-treated pristine SWCNT. The differences of degradation in two carbon nanomaterials are supposed to result from their distinct physicochemical properties. X-ray photoelectron spectroscopy (XPS) and thermogravimetric analysis (TGA) evidenced that a number of oxygen-containing functional groups are present in graphene, likely providing the catalytic sites for the peroxidase action thus facilitating its degradation, as previously demonstrated using other types of oxidative conditions.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/401518
https://api.elsevier.com/content/abstract/scopus_id/105015149786
url http://hdl.handle.net/10261/401518
https://api.elsevier.com/content/abstract/scopus_id/105015149786
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/953152
Journal of Environmental Chemical Engineering
The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1016/j.jece.2025.118671
https://doi.org/10.1016/j.jece.2025.118671
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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