Improving virus production through quasispecies genomic selection and molecular breedings

Virus production still is a challenging issue in antigen manufacture, particularly with slow-growing viruses. Deep-sequencing of genomic regions indicative of efficient replication may be used to identify high-fitness minority individuals suppressed by the ensemble of mutants in a virus quasispecies...

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Autores: Pérez-Rodríguez, Francisco Javier, D'Andrea Rodríguez-Vida, Lucía, Castellarnau Serra, Montserrat de, Costafreda Salvany, M. Isabel (Maria Isabel), Guix Arnau, Susana, Ribes Mora, Enric, Quer, Josep, Gregori Font, Josep, Bosch, Albert, Pintó Solé, Rosa María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/116589
Acceso en línea:https://hdl.handle.net/2445/116589
Access Level:acceso abierto
Palabra clave:Virologia
Espècies (Biologia)
Virology
Species
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spelling Improving virus production through quasispecies genomic selection and molecular breedingsPérez-Rodríguez, Francisco JavierD'Andrea Rodríguez-Vida, LucíaCastellarnau Serra, Montserrat deCostafreda Salvany, M. Isabel (Maria Isabel)Guix Arnau, SusanaRibes Mora, EnricQuer, JosepGregori Font, JosepBosch, AlbertPintó Solé, Rosa MaríaVirologiaEspècies (Biologia)VirologySpeciesVirus production still is a challenging issue in antigen manufacture, particularly with slow-growing viruses. Deep-sequencing of genomic regions indicative of efficient replication may be used to identify high-fitness minority individuals suppressed by the ensemble of mutants in a virus quasispecies. Molecular breeding of quasispecies containing colonizer individuals, under regimes allowing more than one replicative cycle, is a strategy to select the fittest competitors among the colonizers. A slow-growing cell culture-adapted hepatitis A virus strain was employed as a model for this strategy. Using genomic selection in two regions predictive of efficient translation, the internal ribosome entry site and the VP1-coding region, high-fitness minority colonizer individuals were identified in a population adapted to conditions of artificially-induced cellular transcription shut-off. Molecular breeding of this population with a second one, also adapted to transcription shut-off and showing an overall colonizer phenotype, allowed the selection of a fast-growing population of great biotechnological potential.Nature Publishing Group2017201720162017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion12 p.application/pdfhttps://hdl.handle.net/2445/116589Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1038/srep35962Scientific Reports, 2016, vol. 6, p. 35962https://doi.org/10.1038/srep35962cc-by (c) Pérez et al., 2016http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1165892026-05-29T05:05:01Z
dc.title.none.fl_str_mv Improving virus production through quasispecies genomic selection and molecular breedings
title Improving virus production through quasispecies genomic selection and molecular breedings
spellingShingle Improving virus production through quasispecies genomic selection and molecular breedings
Pérez-Rodríguez, Francisco Javier
Virologia
Espècies (Biologia)
Virology
Species
title_short Improving virus production through quasispecies genomic selection and molecular breedings
title_full Improving virus production through quasispecies genomic selection and molecular breedings
title_fullStr Improving virus production through quasispecies genomic selection and molecular breedings
title_full_unstemmed Improving virus production through quasispecies genomic selection and molecular breedings
title_sort Improving virus production through quasispecies genomic selection and molecular breedings
dc.creator.none.fl_str_mv Pérez-Rodríguez, Francisco Javier
D'Andrea Rodríguez-Vida, Lucía
Castellarnau Serra, Montserrat de
Costafreda Salvany, M. Isabel (Maria Isabel)
Guix Arnau, Susana
Ribes Mora, Enric
Quer, Josep
Gregori Font, Josep
Bosch, Albert
Pintó Solé, Rosa María
author Pérez-Rodríguez, Francisco Javier
author_facet Pérez-Rodríguez, Francisco Javier
D'Andrea Rodríguez-Vida, Lucía
Castellarnau Serra, Montserrat de
Costafreda Salvany, M. Isabel (Maria Isabel)
Guix Arnau, Susana
Ribes Mora, Enric
Quer, Josep
Gregori Font, Josep
Bosch, Albert
Pintó Solé, Rosa María
author_role author
author2 D'Andrea Rodríguez-Vida, Lucía
Castellarnau Serra, Montserrat de
Costafreda Salvany, M. Isabel (Maria Isabel)
Guix Arnau, Susana
Ribes Mora, Enric
Quer, Josep
Gregori Font, Josep
Bosch, Albert
Pintó Solé, Rosa María
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Virologia
Espècies (Biologia)
Virology
Species
topic Virologia
Espècies (Biologia)
Virology
Species
description Virus production still is a challenging issue in antigen manufacture, particularly with slow-growing viruses. Deep-sequencing of genomic regions indicative of efficient replication may be used to identify high-fitness minority individuals suppressed by the ensemble of mutants in a virus quasispecies. Molecular breeding of quasispecies containing colonizer individuals, under regimes allowing more than one replicative cycle, is a strategy to select the fittest competitors among the colonizers. A slow-growing cell culture-adapted hepatitis A virus strain was employed as a model for this strategy. Using genomic selection in two regions predictive of efficient translation, the internal ribosome entry site and the VP1-coding region, high-fitness minority colonizer individuals were identified in a population adapted to conditions of artificially-induced cellular transcription shut-off. Molecular breeding of this population with a second one, also adapted to transcription shut-off and showing an overall colonizer phenotype, allowed the selection of a fast-growing population of great biotechnological potential.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/116589
url https://hdl.handle.net/2445/116589
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1038/srep35962
Scientific Reports, 2016, vol. 6, p. 35962
https://doi.org/10.1038/srep35962
dc.rights.none.fl_str_mv cc-by (c) Pérez et al., 2016
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Pérez et al., 2016
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12 p.
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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