Enzymatic removal of free and conjugated sterols forming pitch deposits in environmentally sound bleaching of eucalypt paper pulp

Instituto de Recursos Naturales y Agrobiología, CSIC, PO Box 1052, E-41080, Seville, Spain; Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, E-28040 Madrid, Spain; CIT, ENCE, Carretera de Campañó s/n, E-36157 Ribeiro Vao, Pontevedra, Spain

Bibliographic Details
Authors: Gutiérrez Suárez, Ana, Río Andrade, José Carlos del, Ibarra, David, Rencoret, Jorge, Romero Sánchez, Javier, Speranza, Mariela, Camarero, Susana, Martínez, María Jesús, Martínez, Ángel T.
Format: article
Publication Date:2006
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/39907
Online Access:http://hdl.handle.net/10261/39907
Access Level:Open access
Keyword:Pitch
Sterols
Paper pulp
Laccases
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repository_id_str
spelling Enzymatic removal of free and conjugated sterols forming pitch deposits in environmentally sound bleaching of eucalypt paper pulpGutiérrez Suárez, AnaRío Andrade, José Carlos delIbarra, DavidRencoret, JorgeRomero Sánchez, JavierSperanza, MarielaCamarero, SusanaMartínez, María JesúsMartínez, Ángel T.PitchSterolsPaper pulpLaccasesInstituto de Recursos Naturales y Agrobiología, CSIC, PO Box 1052, E-41080, Seville, Spain; Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, E-28040 Madrid, Spain; CIT, ENCE, Carretera de Campañó s/n, E-36157 Ribeiro Vao, Pontevedra, SpainFree and conjugated sterols are among the main compounds responsible for pitch deposition in the manufacture of wood chemical pulps, making difficult the implementation of totally chlorine free bleaching (TCF) and closure of bleach plant circuits. In this work, the suitability of oxidative enzymes in efficiently removing sterols from eucalypt pulps is revealed. The enzymatic treatment was applied as an additional stage of an industrial-type TCF sequence for bleaching eucalypt kraft pulp. The pulp obtained after oxygen delignification was treated with a highredox potential and thermostable fungal laccase using 1-hydroxybenzotriazole as an enzyme mediator. This pulp was further submitted to chelation and peroxide stages and compared with a control TCF pulp obtained using chemical reagents. The composition of the lipophilic extractives in the pulps and the corresponding liquids after the different stages was analyzed by gas chromatography and gas chromatography-mass spectrometry. Free sitosterol and sitosterol esters and glucosides, the major lipophilic compounds in eucalypt pulps, were completely removed during the laccase-mediator treatment. Only some intermediate products from sitosterol oxidation remained after the laccase stage, as well as in the final pulp. Pulp brightness was also improved due to the simultaneous removal of lignin by the laccase-mediator treatment.This study has been funded by the Spanish projects AGL2002-393 and BIO2002-1166, the EU projects "New environmentally-sound methods for pitch control in different paper pulp manufacturing processes" (contract QLK5-99-1357) and "Fungal metalloenzymes oxidizing aromatic compounds of industrial interest" (contract QLK3-99-590), and two ENCE-CSIC contracts.Peer reviewedAmerican Chemical SocietyEnce Energía y CelulosaConsejo Superior de Investigaciones Científicas (España)European Commission2006info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/39907reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/399072026-05-22T06:33:51Z
dc.title.none.fl_str_mv Enzymatic removal of free and conjugated sterols forming pitch deposits in environmentally sound bleaching of eucalypt paper pulp
title Enzymatic removal of free and conjugated sterols forming pitch deposits in environmentally sound bleaching of eucalypt paper pulp
spellingShingle Enzymatic removal of free and conjugated sterols forming pitch deposits in environmentally sound bleaching of eucalypt paper pulp
Gutiérrez Suárez, Ana
Pitch
Sterols
Paper pulp
Laccases
title_short Enzymatic removal of free and conjugated sterols forming pitch deposits in environmentally sound bleaching of eucalypt paper pulp
title_full Enzymatic removal of free and conjugated sterols forming pitch deposits in environmentally sound bleaching of eucalypt paper pulp
title_fullStr Enzymatic removal of free and conjugated sterols forming pitch deposits in environmentally sound bleaching of eucalypt paper pulp
title_full_unstemmed Enzymatic removal of free and conjugated sterols forming pitch deposits in environmentally sound bleaching of eucalypt paper pulp
title_sort Enzymatic removal of free and conjugated sterols forming pitch deposits in environmentally sound bleaching of eucalypt paper pulp
dc.creator.none.fl_str_mv Gutiérrez Suárez, Ana
Río Andrade, José Carlos del
Ibarra, David
Rencoret, Jorge
Romero Sánchez, Javier
Speranza, Mariela
Camarero, Susana
Martínez, María Jesús
Martínez, Ángel T.
author Gutiérrez Suárez, Ana
author_facet Gutiérrez Suárez, Ana
Río Andrade, José Carlos del
Ibarra, David
Rencoret, Jorge
Romero Sánchez, Javier
Speranza, Mariela
Camarero, Susana
Martínez, María Jesús
Martínez, Ángel T.
author_role author
author2 Río Andrade, José Carlos del
Ibarra, David
Rencoret, Jorge
Romero Sánchez, Javier
Speranza, Mariela
Camarero, Susana
Martínez, María Jesús
Martínez, Ángel T.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ence Energía y Celulosa
Consejo Superior de Investigaciones Científicas (España)
European Commission
dc.subject.none.fl_str_mv Pitch
Sterols
Paper pulp
Laccases
topic Pitch
Sterols
Paper pulp
Laccases
description Instituto de Recursos Naturales y Agrobiología, CSIC, PO Box 1052, E-41080, Seville, Spain; Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, E-28040 Madrid, Spain; CIT, ENCE, Carretera de Campañó s/n, E-36157 Ribeiro Vao, Pontevedra, Spain
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/39907
url http://hdl.handle.net/10261/39907
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869424327950073856
score 15.81155

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