Cracking the code of 3' ss selection in s.cerevisiae

The informational content of 3' splice sites is low and the mechanisms whereby they are selected are not clear. Here we enunciate a set of rules that govern their selection. For many introns, secondary structures are a key factor, because they occlude alternative 3'ss from the spliceosome...

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Detalles Bibliográficos
Autor: Meyer, Markus
Tipo de recurso: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2010
País:España
Institución:CBUC, CESCA
Repositorio:TDR. Tesis Doctorales en Red
OAI Identifier:oai:www.tdx.cat:10803/7232
Acceso en línea:http://www.tdx.cat/TDX-0217111-091859
http://hdl.handle.net/10803/7232
Access Level:acceso abierto
Palabra clave:Saccharomyces cerevisiae
regulació genética
ARN empalmament
rearrangement
U2 snRNP
U1 snRNP
Cbp80
RPL30
L30
splicing regulation
secondary structure
selection
Branch site
3' splice site
5' splice site
Intron
mRNA
spliceosome
Pre-mRNA
splicing
577
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oai_identifier_str oai:www.tdx.cat:10803/7232
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spelling Cracking the code of 3' ss selection in s.cerevisiaeMeyer, MarkusSaccharomyces cerevisiaeregulació genéticaARN empalmamentrearrangementU2 snRNPU1 snRNPCbp80RPL30L30splicing regulationsecondary structureselectionBranch site3' splice site5' splice siteIntronmRNAspliceosomePre-mRNAsplicing577The informational content of 3' splice sites is low and the mechanisms whereby they are selected are not clear. Here we enunciate a set of rules that govern their selection. For many introns, secondary structures are a key factor, because they occlude alternative 3'ss from the spliceosome and reduce the effective distance between the BS and the 3'ss to a maximum of 45 nucleotides. Further alternative 3'ss are disregarded by the spliceosome because they lie at 9 nucleotides or less from the branch site, or because they are weak splice sites. With these rules, we are able to explain the splicing pattern of the vast majority of introns in Saccharomyces cerevisiae. When in excess, L30 blocks the splicing of its own transcript by interfering with a critical rearrangement that is required for the proper recognition of the intron 3' end, and thus for splicing to proceed. We show that the protein Cbp80 has a role in promoting this rearrangement and therefore antagonizes splicing regulation by L30.Tanto la información que define el sitio de splicing 3' como los mecanismos de selección del mismo son poco conocidos. En este trabajo, proponemos una serie de reglas que gobiernan esta selección. Las estructuras secundarias son claves en el caso de muchos intrones, porque son capaces de ocultar sitios de splicing alternativos 3' al spliceosoma, y además reducen la distancia efectiva entre el punto de ramificación y el sitio de splicing 3' a un máximo de 45 nucleotidos. Otros sitios de splicing alternativo 3' no son considerados por el spliceosoma como tales porque se encuentran a 9 nucleotidos o menos del punto de ramificación, o porque son sitios de splicing débiles. Con estas reglas somos capaces de explicar el splicing de la mayoría de intrones de Saccharomyces cerevisiae. El exceso de proteína L30 bloquea el splicing de su propio tránscrito porque interfiere con la reorganización necesaria para el correcto reconocimiento del 3' final del intrón, y por tanto de su splicing. Demostramos que la proteína Cbp80 está implicada en promover esta reorganización y que por tanto antagoniza la regulación del splicing por L30.Programa de doctorat en BiomedicinaUniversitat Pompeu FabraVilardell, JosepUniversitat Pompeu Fabra. Departament de Ciències Experimentals i de la Salut2011201120102011info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://www.tdx.cat/TDX-0217111-091859http://hdl.handle.net/10803/7232TDX (Tesis Doctorals en Xarxa)reponame:TDR. Tesis Doctorales en Redinstname:CBUC, CESCAInglésADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs.info:eu-repo/semantics/openAccessoai:www.tdx.cat:10803/72322026-06-14T12:46:07Z
dc.title.none.fl_str_mv Cracking the code of 3' ss selection in s.cerevisiae
title Cracking the code of 3' ss selection in s.cerevisiae
spellingShingle Cracking the code of 3' ss selection in s.cerevisiae
Meyer, Markus
Saccharomyces cerevisiae
regulació genética
ARN empalmament
rearrangement
U2 snRNP
U1 snRNP
Cbp80
RPL30
L30
splicing regulation
secondary structure
selection
Branch site
3' splice site
5' splice site
Intron
mRNA
spliceosome
Pre-mRNA
splicing
577
title_short Cracking the code of 3' ss selection in s.cerevisiae
title_full Cracking the code of 3' ss selection in s.cerevisiae
title_fullStr Cracking the code of 3' ss selection in s.cerevisiae
title_full_unstemmed Cracking the code of 3' ss selection in s.cerevisiae
title_sort Cracking the code of 3' ss selection in s.cerevisiae
dc.creator.none.fl_str_mv Meyer, Markus
author Meyer, Markus
author_facet Meyer, Markus
author_role author
dc.contributor.none.fl_str_mv Vilardell, Josep
Universitat Pompeu Fabra. Departament de Ciències Experimentals i de la Salut
dc.subject.none.fl_str_mv Saccharomyces cerevisiae
regulació genética
ARN empalmament
rearrangement
U2 snRNP
U1 snRNP
Cbp80
RPL30
L30
splicing regulation
secondary structure
selection
Branch site
3' splice site
5' splice site
Intron
mRNA
spliceosome
Pre-mRNA
splicing
577
topic Saccharomyces cerevisiae
regulació genética
ARN empalmament
rearrangement
U2 snRNP
U1 snRNP
Cbp80
RPL30
L30
splicing regulation
secondary structure
selection
Branch site
3' splice site
5' splice site
Intron
mRNA
spliceosome
Pre-mRNA
splicing
577
description The informational content of 3' splice sites is low and the mechanisms whereby they are selected are not clear. Here we enunciate a set of rules that govern their selection. For many introns, secondary structures are a key factor, because they occlude alternative 3'ss from the spliceosome and reduce the effective distance between the BS and the 3'ss to a maximum of 45 nucleotides. Further alternative 3'ss are disregarded by the spliceosome because they lie at 9 nucleotides or less from the branch site, or because they are weak splice sites. With these rules, we are able to explain the splicing pattern of the vast majority of introns in Saccharomyces cerevisiae. When in excess, L30 blocks the splicing of its own transcript by interfering with a critical rearrangement that is required for the proper recognition of the intron 3' end, and thus for splicing to proceed. We show that the protein Cbp80 has a role in promoting this rearrangement and therefore antagonizes splicing regulation by L30.
publishDate 2010
dc.date.none.fl_str_mv 2010
2011
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv http://www.tdx.cat/TDX-0217111-091859
http://hdl.handle.net/10803/7232
url http://www.tdx.cat/TDX-0217111-091859
http://hdl.handle.net/10803/7232
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universitat Pompeu Fabra
publisher.none.fl_str_mv Universitat Pompeu Fabra
dc.source.none.fl_str_mv TDX (Tesis Doctorals en Xarxa)
reponame:TDR. Tesis Doctorales en Red
instname:CBUC, CESCA
instname_str CBUC, CESCA
reponame_str TDR. Tesis Doctorales en Red
collection TDR. Tesis Doctorales en Red
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869424301998866432
score 15,300724

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