Cracking the code of 3' ss selection in s.cerevisiae
The informational content of 3' splice sites is low and the mechanisms whereby they are selected are not clear. Here we enunciate a set of rules that govern their selection. For many introns, secondary structures are a key factor, because they occlude alternative 3'ss from the spliceosome...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2010 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/7232 |
| Acceso en línea: | http://www.tdx.cat/TDX-0217111-091859 http://hdl.handle.net/10803/7232 |
| Access Level: | acceso abierto |
| Palabra clave: | Saccharomyces cerevisiae regulació genética ARN empalmament rearrangement U2 snRNP U1 snRNP Cbp80 RPL30 L30 splicing regulation secondary structure selection Branch site 3' splice site 5' splice site Intron mRNA spliceosome Pre-mRNA splicing 577 |
| Sumario: | The informational content of 3' splice sites is low and the mechanisms whereby they are selected are not clear. Here we enunciate a set of rules that govern their selection. For many introns, secondary structures are a key factor, because they occlude alternative 3'ss from the spliceosome and reduce the effective distance between the BS and the 3'ss to a maximum of 45 nucleotides. Further alternative 3'ss are disregarded by the spliceosome because they lie at 9 nucleotides or less from the branch site, or because they are weak splice sites. With these rules, we are able to explain the splicing pattern of the vast majority of introns in Saccharomyces cerevisiae. When in excess, L30 blocks the splicing of its own transcript by interfering with a critical rearrangement that is required for the proper recognition of the intron 3' end, and thus for splicing to proceed. We show that the protein Cbp80 has a role in promoting this rearrangement and therefore antagonizes splicing regulation by L30. |
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