The computer-controlled multicompartmental dynamic model of the gastrointestinal system SIMGI

The SIMGI (SIMulator Gastro-Intestinal) is an automated gastrointestinal in vitro model designed to dynamically simulate the physiological processes taking place during digestion in the stomach and small intestine, as well as to reproduce the colonic microbiota responsible for metabolic bioconversio...

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Detalhes bibliográficos
Autores: Barroso, Elvira, Cueva, Carolina, Peláez, Carmen, Martínez-Cuesta, M. Carmen, Requena, Teresa
Formato: otro
Estado:Versión publicada
Fecha de publicación:2015
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/151895
Acesso em linha:http://hdl.handle.net/10261/151895
Access Level:acceso abierto
Palavra-chave:Three-stage fermentation
SIMGI
Automated in vitro dynamic model
Colon microbiota
Descrição
Resumo:The SIMGI (SIMulator Gastro-Intestinal) is an automated gastrointestinal in vitro model designed to dynamically simulate the physiological processes taking place during digestion in the stomach and small intestine, as well as to reproduce the colonic microbiota responsible for metabolic bioconversions in the large intestine. This computer-controlled system is a flexible modulating system that combines a gastric compartment that operates with peristaltic mixing movements, a reactor simulating the small intestine and three-stage continuous reactors that reproduce the colon region-specific microbiota. The compartments designed for digestion (stomach and small intestine) and fermentation (colon) can be connected to operate jointly. Alternatively, the digestion and fermentation processes can proceed independently. This section describes the conditions needed to inoculate, stabilize and differentiate the fecal microbiota in the SIMGI system, as well as the steps to follow in order to test the stabilized colonic microbiota with different food ingredients and/or by modifying the caloric intake in the nutrition media.