Comparative Analysis of TCR-gamma Gene Rearrangements by Genescan and Polyacrylamide Gel-electrophoresis in Cutaneous T-cell Lymphoma

Demonstrating T-cell clonality has become an important approach supporting a diagnosis of malignant T-cell neoplasms. A comparative study between Genescan analysis, polyacrylamide gel and agarose gel electrophoresis in visualizing X-cell receptor gamma gene rearrangement was performed on 25 biopsy s...

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Autores: Costa, Carlota, Gallardo, F. (Fernando), Pujol, Ramon M., Espinet Solà, Blanca, Bellosillo, Beatriz, Estrach Panella, Ma. Teresa (María Teresa), Servitje Bedate, Octavio, Barranco, Carlos, Serrano, Sergi, Solé Ristol, Francesc
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2004
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/127629
Acceso en línea:https://hdl.handle.net/2445/127629
Access Level:acceso abierto
Palabra clave:Limfomes
Cèl·lules T
Lymphomas
T cells
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spelling Comparative Analysis of TCR-gamma Gene Rearrangements by Genescan and Polyacrylamide Gel-electrophoresis in Cutaneous T-cell LymphomaCosta, CarlotaGallardo, F. (Fernando)Pujol, Ramon M.Espinet Solà, BlancaBellosillo, BeatrizEstrach Panella, Ma. Teresa (María Teresa)Servitje Bedate, OctavioBarranco, CarlosSerrano, SergiSolé Ristol, FrancescLimfomesCèl·lules TLymphomasT cellsDemonstrating T-cell clonality has become an important approach supporting a diagnosis of malignant T-cell neoplasms. A comparative study between Genescan analysis, polyacrylamide gel and agarose gel electrophoresis in visualizing X-cell receptor gamma gene rearrangement was performed on 25 biopsy specimens from 18 patients with different forms of cutaneous T-cell lymphomas. Clonality was detected in 17 biopsy specimens when PCR products were evaluated by Genescan analysis. Seventeen showed discrete bands when visualized in polyacrylamide gel and 14 cases were clonal when visualized with agarose gel. In five cases, a clonal population was seen in the gels, but not with Genescan. On sequencing the PCR products we demonstrated nonclonality of these five samples. Our results confirm that PCR-Genescan is a useful, reliable and specific screening method for detecting dominant clones in patients with T-cell lymphoma.Society for the Publication of Acta Dermato-Venereologica2004info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/127629Articles publicats en revistes (Ciències Clíniques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1080/00015550310015798Acta Dermato-Venereologica, 2004, vol. 84, num. 1, p. 6-11https://doi.org/10.1080/00015550310015798(c) Costa, Carlota et al., 2004info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1276292026-05-27T06:46:51Z
dc.title.none.fl_str_mv Comparative Analysis of TCR-gamma Gene Rearrangements by Genescan and Polyacrylamide Gel-electrophoresis in Cutaneous T-cell Lymphoma
title Comparative Analysis of TCR-gamma Gene Rearrangements by Genescan and Polyacrylamide Gel-electrophoresis in Cutaneous T-cell Lymphoma
spellingShingle Comparative Analysis of TCR-gamma Gene Rearrangements by Genescan and Polyacrylamide Gel-electrophoresis in Cutaneous T-cell Lymphoma
Costa, Carlota
Limfomes
Cèl·lules T
Lymphomas
T cells
title_short Comparative Analysis of TCR-gamma Gene Rearrangements by Genescan and Polyacrylamide Gel-electrophoresis in Cutaneous T-cell Lymphoma
title_full Comparative Analysis of TCR-gamma Gene Rearrangements by Genescan and Polyacrylamide Gel-electrophoresis in Cutaneous T-cell Lymphoma
title_fullStr Comparative Analysis of TCR-gamma Gene Rearrangements by Genescan and Polyacrylamide Gel-electrophoresis in Cutaneous T-cell Lymphoma
title_full_unstemmed Comparative Analysis of TCR-gamma Gene Rearrangements by Genescan and Polyacrylamide Gel-electrophoresis in Cutaneous T-cell Lymphoma
title_sort Comparative Analysis of TCR-gamma Gene Rearrangements by Genescan and Polyacrylamide Gel-electrophoresis in Cutaneous T-cell Lymphoma
dc.creator.none.fl_str_mv Costa, Carlota
Gallardo, F. (Fernando)
Pujol, Ramon M.
Espinet Solà, Blanca
Bellosillo, Beatriz
Estrach Panella, Ma. Teresa (María Teresa)
Servitje Bedate, Octavio
Barranco, Carlos
Serrano, Sergi
Solé Ristol, Francesc
author Costa, Carlota
author_facet Costa, Carlota
Gallardo, F. (Fernando)
Pujol, Ramon M.
Espinet Solà, Blanca
Bellosillo, Beatriz
Estrach Panella, Ma. Teresa (María Teresa)
Servitje Bedate, Octavio
Barranco, Carlos
Serrano, Sergi
Solé Ristol, Francesc
author_role author
author2 Gallardo, F. (Fernando)
Pujol, Ramon M.
Espinet Solà, Blanca
Bellosillo, Beatriz
Estrach Panella, Ma. Teresa (María Teresa)
Servitje Bedate, Octavio
Barranco, Carlos
Serrano, Sergi
Solé Ristol, Francesc
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Limfomes
Cèl·lules T
Lymphomas
T cells
topic Limfomes
Cèl·lules T
Lymphomas
T cells
description Demonstrating T-cell clonality has become an important approach supporting a diagnosis of malignant T-cell neoplasms. A comparative study between Genescan analysis, polyacrylamide gel and agarose gel electrophoresis in visualizing X-cell receptor gamma gene rearrangement was performed on 25 biopsy specimens from 18 patients with different forms of cutaneous T-cell lymphomas. Clonality was detected in 17 biopsy specimens when PCR products were evaluated by Genescan analysis. Seventeen showed discrete bands when visualized in polyacrylamide gel and 14 cases were clonal when visualized with agarose gel. In five cases, a clonal population was seen in the gels, but not with Genescan. On sequencing the PCR products we demonstrated nonclonality of these five samples. Our results confirm that PCR-Genescan is a useful, reliable and specific screening method for detecting dominant clones in patients with T-cell lymphoma.
publishDate 2004
dc.date.none.fl_str_mv 2004
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/127629
url https://hdl.handle.net/2445/127629
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1080/00015550310015798
Acta Dermato-Venereologica, 2004, vol. 84, num. 1, p. 6-11
https://doi.org/10.1080/00015550310015798
dc.rights.none.fl_str_mv (c) Costa, Carlota et al., 2004
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Costa, Carlota et al., 2004
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Society for the Publication of Acta Dermato-Venereologica
publisher.none.fl_str_mv Society for the Publication of Acta Dermato-Venereologica
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Clíniques)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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