Toward Kilogram-Scale Peroxygenase-Catalyzed Oxyfunctionalization of Cyclohexane

Mol-scale oxyfunctionalization of cyclohexane to cyclohexanol/cyclohexanone (KA-oil) using an unspecific peroxygenase is reported. Using AaeUPO from Agrocybe aegerita and simple H2O2 as an oxidant, cyclohexanol concentrations of more than 300 mM (>60% yield) at attractive productivities (157 mM h...

Descripción completa

Detalles Bibliográficos
Autores: Hilberath, T., van Oosten, R., Victoria, J., Brasselet, H., Alcalde Galeote, Miguel, Woodley, J.M., Hollmann, F.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/359313
Acceso en línea:http://hdl.handle.net/10261/359313
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85163489019&doi=10.1021%2facs.oprd.3c00135&partnerID=40&md5=ecbb738ad4012d457c5ad5a817594be1
Access Level:acceso abierto
Palabra clave:Biocatalysis
bulk chemical
cyclohexane
oxyfunctionalization
peroxygenase
upscaling
Descripción
Sumario:Mol-scale oxyfunctionalization of cyclohexane to cyclohexanol/cyclohexanone (KA-oil) using an unspecific peroxygenase is reported. Using AaeUPO from Agrocybe aegerita and simple H2O2 as an oxidant, cyclohexanol concentrations of more than 300 mM (>60% yield) at attractive productivities (157 mM h-1, approx. 15 g L-1 h-1) were achieved. Current limitations of the proposed biooxidation system have been identified paving the way for future improvements and implementation. © 2023 The Authors. Published by American Chemical Society