In vitro establishment and micropropagation of mango (Mangifera indica L.) from cotyledonary nodes

Mango is the fifth most important fruit crop in the world in terms of production with an increasing demand for high-quality plant material for new plantings. Compared to other fruit tree crops, vegetative propagation in mango is slow and allows only a relatively limited production of plant material....

Descripción completa

Detalles Bibliográficos
Autores: Conde, Francisco, Carmona-Martin, Elizabeth, Hormaza, Jose I., Petri, Cesar
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/372027
Acceso en línea:http://hdl.handle.net/10261/372027
https://api.elsevier.com/content/abstract/scopus_id/85148654887
Access Level:acceso abierto
Palabra clave:Anacardiaceae | Fruit crops | Germination | Organogenesis | Shoot multiplication | Shoot regeneration
id ES_f07ab2fba8a767f3c3c8f0a83cfed566
oai_identifier_str oai:digital.csic.es:10261/372027
network_acronym_str ES
network_name_str España
repository_id_str
spelling In vitro establishment and micropropagation of mango (Mangifera indica L.) from cotyledonary nodesConde, FranciscoCarmona-Martin, ElizabethHormaza, Jose I.Petri, CesarAnacardiaceae | Fruit crops | Germination | Organogenesis | Shoot multiplication | Shoot regenerationMango is the fifth most important fruit crop in the world in terms of production with an increasing demand for high-quality plant material for new plantings. Compared to other fruit tree crops, vegetative propagation in mango is slow and allows only a relatively limited production of plant material. To date, efficient procedures for in vitro establishment and micropropagation are not yet available in mango. This work aims at filling this gap. Germination of mango seeds in vitro, compared with ex vitro conditions, significantly increased the germination rate for the monoembryonic genotype tested (‘Irwin’). In vitro germination also increased the number of developed embryos for the two polyembryonic genotypes analysed, ‘Ataulfo’ and ‘Gomera-4’. Regarding the use of shoot tips for in vitro establishment and micropropagation, our results confirmed that this explant is not adequate for this purpose. We report for the first time the use of cotyledonary nodes as initial explants in mango. Axillary shoots were obtained in all the genotypes tested (‘Ataulfo’, ‘Sabre’, ‘Gomera-4’, ‘Irwin’ and ‘Keitt’), although the regeneration rate was highly genotype-dependent. Thidiazuron induced high-frequency regeneration patterns. The best results were observed with ‘Keitt’. When 3.0 mg l−1 thidiazuron was added to the medium, a 63.15% regeneration rate was reached and about 4 shoots per regenerating explant were obtained. Subsequently, microshoots excised from the cotyledonary nodes were successfully rooted in vitro and acclimatized to ex vitro conditions. Our results show that the use of cotyledonary nodes is efficient for mango mass propagation and, consequently, represents a qualitative advance for in vitro propagation of this recalcitrant species.This study was funded by Junta de Andalucía co-financed by FEDER funds (project reference P18-RT-3272) and by Agencia Estatal de Investigación – Ministerio de Ciencia e Innovación (Spain) (project reference PID2019-109566RB-I00/AEIPeer reviewedSpringerJunta de AndalucíaMinisterio de Ciencia e Innovación (España)European CommissionAgencia Estatal de Investigación (España)0000-0003-0773-929XConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/372027https://api.elsevier.com/content/abstract/scopus_id/85148654887reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109566RB-I00In Vitro Cellular and Developmental Biology - Planthttps://doi.org/10.13039/501100011033)Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3720272026-05-22T06:33:51Z
dc.title.none.fl_str_mv In vitro establishment and micropropagation of mango (Mangifera indica L.) from cotyledonary nodes
title In vitro establishment and micropropagation of mango (Mangifera indica L.) from cotyledonary nodes
spellingShingle In vitro establishment and micropropagation of mango (Mangifera indica L.) from cotyledonary nodes
Conde, Francisco
Anacardiaceae | Fruit crops | Germination | Organogenesis | Shoot multiplication | Shoot regeneration
title_short In vitro establishment and micropropagation of mango (Mangifera indica L.) from cotyledonary nodes
title_full In vitro establishment and micropropagation of mango (Mangifera indica L.) from cotyledonary nodes
title_fullStr In vitro establishment and micropropagation of mango (Mangifera indica L.) from cotyledonary nodes
title_full_unstemmed In vitro establishment and micropropagation of mango (Mangifera indica L.) from cotyledonary nodes
title_sort In vitro establishment and micropropagation of mango (Mangifera indica L.) from cotyledonary nodes
dc.creator.none.fl_str_mv Conde, Francisco
Carmona-Martin, Elizabeth
Hormaza, Jose I.
Petri, Cesar
author Conde, Francisco
author_facet Conde, Francisco
Carmona-Martin, Elizabeth
Hormaza, Jose I.
Petri, Cesar
author_role author
author2 Carmona-Martin, Elizabeth
Hormaza, Jose I.
Petri, Cesar
author2_role author
author
author
dc.contributor.none.fl_str_mv Junta de Andalucía
Ministerio de Ciencia e Innovación (España)
European Commission
Agencia Estatal de Investigación (España)
0000-0003-0773-929X
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Anacardiaceae | Fruit crops | Germination | Organogenesis | Shoot multiplication | Shoot regeneration
topic Anacardiaceae | Fruit crops | Germination | Organogenesis | Shoot multiplication | Shoot regeneration
description Mango is the fifth most important fruit crop in the world in terms of production with an increasing demand for high-quality plant material for new plantings. Compared to other fruit tree crops, vegetative propagation in mango is slow and allows only a relatively limited production of plant material. To date, efficient procedures for in vitro establishment and micropropagation are not yet available in mango. This work aims at filling this gap. Germination of mango seeds in vitro, compared with ex vitro conditions, significantly increased the germination rate for the monoembryonic genotype tested (‘Irwin’). In vitro germination also increased the number of developed embryos for the two polyembryonic genotypes analysed, ‘Ataulfo’ and ‘Gomera-4’. Regarding the use of shoot tips for in vitro establishment and micropropagation, our results confirmed that this explant is not adequate for this purpose. We report for the first time the use of cotyledonary nodes as initial explants in mango. Axillary shoots were obtained in all the genotypes tested (‘Ataulfo’, ‘Sabre’, ‘Gomera-4’, ‘Irwin’ and ‘Keitt’), although the regeneration rate was highly genotype-dependent. Thidiazuron induced high-frequency regeneration patterns. The best results were observed with ‘Keitt’. When 3.0 mg l−1 thidiazuron was added to the medium, a 63.15% regeneration rate was reached and about 4 shoots per regenerating explant were obtained. Subsequently, microshoots excised from the cotyledonary nodes were successfully rooted in vitro and acclimatized to ex vitro conditions. Our results show that the use of cotyledonary nodes is efficient for mango mass propagation and, consequently, represents a qualitative advance for in vitro propagation of this recalcitrant species.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/372027
https://api.elsevier.com/content/abstract/scopus_id/85148654887
url http://hdl.handle.net/10261/372027
https://api.elsevier.com/content/abstract/scopus_id/85148654887
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109566RB-I00
In Vitro Cellular and Developmental Biology - Plant
https://doi.org/10.13039/501100011033)
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869423959963860992
score 15,811543

Similares en LA Referencia