Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis Cells

Berdasco, María; Alcázar Hernández, Rubén; García-Ortiz, María Victoria; Ballestar Tarín, Esteban; Fernández, Agustín F.; Roldán-Arjona, Teresa; Fernández Tiburcio, Antonio; Altabella Artigas, Teresa; Buisine, Nicolas; Quesneville, Hadi; Baudry, Antoine; Lepiniec, Loïc; Alaminos, Miguel; Rodríguez, Roberto; Lloyd, Alan; Colot, Vincent; Bender, Judith; Canal, María Jesús; Esteller, Manel; Fraga, Mario F.

Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis Cells

Maintaining and acquiring the pluripotent cell state in plants is critical to tissue regeneration and vegetative multiplication. Histone-based epigenetic mechanisms are important for regulating this undifferentiated state. Here we report the use of genetic and pharmacological experimental approaches...

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Autores:	Berdasco, María, Alcázar Hernández, Rubén, García-Ortiz, María Victoria, Ballestar Tarín, Esteban, Fernández, Agustín F., Roldán-Arjona, Teresa, Fernández Tiburcio, Antonio, Altabella Artigas, Teresa, Buisine, Nicolas, Quesneville, Hadi, Baudry, Antoine, Lepiniec, Loïc, Alaminos, Miguel, Rodríguez, Roberto, Lloyd, Alan, Colot, Vincent, Bender, Judith, Canal, María Jesús, Esteller, Manel, Fraga, Mario F.
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2008
País:	España
Institución:	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:	Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:	oai:recercat.cat:2445/61866
Acceso en línea:	https://hdl.handle.net/2445/61866
Access Level:	acceso abierto
Palabra clave:	Arabidopsis Metilació ADN Epigènesi Diferenciació cel·lular Methylation DNA Epigenesis Cell diferentiation

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spelling	Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis CellsBerdasco, MaríaAlcázar Hernández, RubénGarcía-Ortiz, María VictoriaBallestar Tarín, EstebanFernández, Agustín F.Roldán-Arjona, TeresaFernández Tiburcio, AntonioAltabella Artigas, TeresaBuisine, NicolasQuesneville, HadiBaudry, AntoineLepiniec, LoïcAlaminos, MiguelRodríguez, RobertoLloyd, AlanColot, VincentBender, JudithCanal, María JesúsEsteller, ManelFraga, Mario F.ArabidopsisMetilacióADNEpigènesiDiferenciació cel·lularArabidopsisMethylationDNAEpigenesisCell diferentiationMaintaining and acquiring the pluripotent cell state in plants is critical to tissue regeneration and vegetative multiplication. Histone-based epigenetic mechanisms are important for regulating this undifferentiated state. Here we report the use of genetic and pharmacological experimental approaches to show that Arabidopsis cell suspensions and calluses specifically repress some genes as a result of promoter DNA hypermethylation. We found that promoters of the MAPK12, GSTU10 and BXL1 genes become hypermethylated in callus cells and that hypermethylation also affects the TTG1, GSTF5, SUVH8, fimbrin and CCD7 genes in cell suspensions. Promoter hypermethylation in undifferentiated cells was associated with histone hypoacetylation and primarily occurred at CpG sites. Accordingly, we found that the process specifically depends on MET1 and DRM2 methyltransferases, as demonstrated with DNA methyltransferase mutants. Our results suggest that promoter DNA methylation may be another important epigenetic mechanism for the establishment and/or maintenance of the undifferentiated state in plant cells.Public Library of Science (PLoS)2015201520082015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion10 p.application/pdfhttps://hdl.handle.net/2445/61866Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0003306PLoS One, 2008, vol. 3, num. 10, p. e3306http://dx.doi.org/10.1371/journal.pone.0003306cc-by (c) Berdasco, María et al., 2008http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/618662026-05-29T05:05:01Z
dc.title.none.fl_str_mv	Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis Cells
title	Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis Cells
spellingShingle	Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis Cells Berdasco, María Arabidopsis Metilació ADN Epigènesi Diferenciació cel·lular Arabidopsis Methylation DNA Epigenesis Cell diferentiation
title_short	Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis Cells
title_full	Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis Cells
title_fullStr	Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis Cells
title_full_unstemmed	Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis Cells
title_sort	Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis Cells
dc.creator.none.fl_str_mv	Berdasco, María Alcázar Hernández, Rubén García-Ortiz, María Victoria Ballestar Tarín, Esteban Fernández, Agustín F. Roldán-Arjona, Teresa Fernández Tiburcio, Antonio Altabella Artigas, Teresa Buisine, Nicolas Quesneville, Hadi Baudry, Antoine Lepiniec, Loïc Alaminos, Miguel Rodríguez, Roberto Lloyd, Alan Colot, Vincent Bender, Judith Canal, María Jesús Esteller, Manel Fraga, Mario F.
author	Berdasco, María
author_facet	Berdasco, María Alcázar Hernández, Rubén García-Ortiz, María Victoria Ballestar Tarín, Esteban Fernández, Agustín F. Roldán-Arjona, Teresa Fernández Tiburcio, Antonio Altabella Artigas, Teresa Buisine, Nicolas Quesneville, Hadi Baudry, Antoine Lepiniec, Loïc Alaminos, Miguel Rodríguez, Roberto Lloyd, Alan Colot, Vincent Bender, Judith Canal, María Jesús Esteller, Manel Fraga, Mario F.
author_role	author
author2	Alcázar Hernández, Rubén García-Ortiz, María Victoria Ballestar Tarín, Esteban Fernández, Agustín F. Roldán-Arjona, Teresa Fernández Tiburcio, Antonio Altabella Artigas, Teresa Buisine, Nicolas Quesneville, Hadi Baudry, Antoine Lepiniec, Loïc Alaminos, Miguel Rodríguez, Roberto Lloyd, Alan Colot, Vincent Bender, Judith Canal, María Jesús Esteller, Manel Fraga, Mario F.
author2_role	author author author author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	Arabidopsis Metilació ADN Epigènesi Diferenciació cel·lular Arabidopsis Methylation DNA Epigenesis Cell diferentiation
topic	Arabidopsis Metilació ADN Epigènesi Diferenciació cel·lular Arabidopsis Methylation DNA Epigenesis Cell diferentiation
description	Maintaining and acquiring the pluripotent cell state in plants is critical to tissue regeneration and vegetative multiplication. Histone-based epigenetic mechanisms are important for regulating this undifferentiated state. Here we report the use of genetic and pharmacological experimental approaches to show that Arabidopsis cell suspensions and calluses specifically repress some genes as a result of promoter DNA hypermethylation. We found that promoters of the MAPK12, GSTU10 and BXL1 genes become hypermethylated in callus cells and that hypermethylation also affects the TTG1, GSTF5, SUVH8, fimbrin and CCD7 genes in cell suspensions. Promoter hypermethylation in undifferentiated cells was associated with histone hypoacetylation and primarily occurred at CpG sites. Accordingly, we found that the process specifically depends on MET1 and DRM2 methyltransferases, as demonstrated with DNA methyltransferase mutants. Our results suggest that promoter DNA methylation may be another important epigenetic mechanism for the establishment and/or maintenance of the undifferentiated state in plant cells.
publishDate	2008
dc.date.none.fl_str_mv	2008 2015 2015 2015
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://hdl.handle.net/2445/61866
url	https://hdl.handle.net/2445/61866
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0003306 PLoS One, 2008, vol. 3, num. 10, p. e3306 http://dx.doi.org/10.1371/journal.pone.0003306
dc.rights.none.fl_str_mv	cc-by (c) Berdasco, María et al., 2008 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess
rights_invalid_str_mv	cc-by (c) Berdasco, María et al., 2008 http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	10 p. application/pdf
dc.publisher.none.fl_str_mv	Public Library of Science (PLoS)
publisher.none.fl_str_mv	Public Library of Science (PLoS)
dc.source.none.fl_str_mv	Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str	Recercat. Dipósit de la Recerca de Catalunya
collection	Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15.811543

Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis Cells

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