Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis Cells

Maintaining and acquiring the pluripotent cell state in plants is critical to tissue regeneration and vegetative multiplication. Histone-based epigenetic mechanisms are important for regulating this undifferentiated state. Here we report the use of genetic and pharmacological experimental approaches...

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Detalles Bibliográficos
Autores: Berdasco, María, Alcázar Hernández, Rubén, García-Ortiz, María Victoria, Ballestar Tarín, Esteban, Fernández, Agustín F., Roldán-Arjona, Teresa, Fernández Tiburcio, Antonio, Altabella Artigas, Teresa, Buisine, Nicolas, Quesneville, Hadi, Baudry, Antoine, Lepiniec, Loïc, Alaminos, Miguel, Rodríguez, Roberto, Lloyd, Alan, Colot, Vincent, Bender, Judith, Canal, María Jesús, Esteller, Manel, Fraga, Mario F.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/61866
Acceso en línea:https://hdl.handle.net/2445/61866
Access Level:acceso abierto
Palabra clave:Arabidopsis
Metilació
ADN
Epigènesi
Diferenciació cel·lular
Methylation
DNA
Epigenesis
Cell diferentiation
Descripción
Sumario:Maintaining and acquiring the pluripotent cell state in plants is critical to tissue regeneration and vegetative multiplication. Histone-based epigenetic mechanisms are important for regulating this undifferentiated state. Here we report the use of genetic and pharmacological experimental approaches to show that Arabidopsis cell suspensions and calluses specifically repress some genes as a result of promoter DNA hypermethylation. We found that promoters of the MAPK12, GSTU10 and BXL1 genes become hypermethylated in callus cells and that hypermethylation also affects the TTG1, GSTF5, SUVH8, fimbrin and CCD7 genes in cell suspensions. Promoter hypermethylation in undifferentiated cells was associated with histone hypoacetylation and primarily occurred at CpG sites. Accordingly, we found that the process specifically depends on MET1 and DRM2 methyltransferases, as demonstrated with DNA methyltransferase mutants. Our results suggest that promoter DNA methylation may be another important epigenetic mechanism for the establishment and/or maintenance of the undifferentiated state in plant cells.

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