Extending the shelf-life of fresh-cut fruit salad combining conventional packaging in polylactic acid double bottom container with dynamic antimicrobial imines of trans-2-hexenal and chitosan

Trans-2-Hexenal (HX) was stabilized in amino-polysaccharide chitosan films via reversible imine formation and were integrated into the aliphatic polyester polylactic acid (PLA) container featuring a perforated double bottom for the preservation of a minimally processed mixed fruit salad. Fruit exuda...

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Detalles Bibliográficos
Autores: Esteve Redondo, Patricia, Heras Mozos, Raquel, López-Carballo, Gracia, Gavara, Rafael, Hernández-Muñoz, Pilar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::c7513a2ec6ea7dffb32023e4432506c7
Acceso en línea:http://hdl.handle.net/10261/428416
https://api.elsevier.com/content/abstract/scopus_id/105034468419
Access Level:acceso abierto
Palabra clave:Chitosan
Fresh-cut fruit
Microbial load
Pathogens
Polylactic acid
Responsive packaging
Trans-2-hexenal
chitosan
pathogens
food packaging
fresh-cut produce
Descripción
Sumario:Trans-2-Hexenal (HX) was stabilized in amino-polysaccharide chitosan films via reversible imine formation and were integrated into the aliphatic polyester polylactic acid (PLA) container featuring a perforated double bottom for the preservation of a minimally processed mixed fruit salad. Fruit exudate triggered imine hydrolysis, enabling controlled release of HX. After 12 days of storage at 6 °C, control samples reached microbial loads of 5.2 log CFU/g for moulds, yeasts and mesophilic bacteria, whereas active packages showed significantly lower count. Moreover, active system significantly reduced inoculated populations of Salmonella enterica, Listeria innocua and Escherichia coli, while effectively preserving quality attributes such as colour in pineapple and melon. By day 12, HX sorbed in the salad reached 3.1 ± 0.4 and 2.0 ± 0.4 μg/g when using 0.5 and 0.25 g of active film, respectively. Toxicological assessment using Caenorhabditis elegans showed 100% survival, confirming the safety of HX levels found in the salad.