Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO)

Gil-Villalba, Sergio; Palau, Jordi; Soder-Walz, Jesica M.; Vallecillo, Miguel A.; Corregidor, Jordi; Tirado, Andrea; Shouakar-Stash, O.; Guivernau, Miriam; Viñas, Miquel; Soler, Anna; Rosell, Mònica

Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO)

Enhanced In Situ Bioremediation (EISB) using Emulsified Vegetable Oil (EVO) as a long-term electron donor has gained prominence for the treatment of groundwater contaminated with chlorinated ethenes (CEs). This study explores the potential of isotopic and molecular biology tools (MBT) to investigate...

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Autores:	Gil-Villalba, Sergio, Palau, Jordi, Soder-Walz, Jesica M., Vallecillo, Miguel A., Corregidor, Jordi, Tirado, Andrea, Shouakar-Stash, O., Guivernau, Miriam, Viñas, Miquel, Soler, Anna, Rosell, Mònica
Tipo de recurso:	artículo
Estado:	Versión aceptada para publicación
Fecha de publicación:	2024
País:	España
Institución:	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:	Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:	oai:recercat.cat:2445/218415
Acceso en línea:	https://hdl.handle.net/2445/218415
Access Level:	acceso abierto
Palabra clave:	Bioremediació Depuració d'aigües residuals Isòtops Contaminació de l'aigua Bioremediation Purification of sewage Isotopes Water pollution

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spelling	Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO)Gil-Villalba, SergioPalau, JordiSoder-Walz, Jesica M.Vallecillo, Miguel A.Corregidor, JordiTirado, AndreaShouakar-Stash, O.Guivernau, MiriamViñas, MiquelSoler, AnnaRosell, MònicaBioremediacióDepuració d'aigües residualsIsòtopsContaminació de l'aiguaBioremediationPurification of sewageIsotopesWater pollutionEnhanced In Situ Bioremediation (EISB) using Emulsified Vegetable Oil (EVO) as a long-term electron donor has gained prominence for the treatment of groundwater contaminated with chlorinated ethenes (CEs). This study explores the potential of isotopic and molecular biology tools (MBT) to investigate the CEs (PCE, TCE and cis-DCE) bioremediation using EVO in a contaminated site. A multiple approach using C and Cl-CSIA, quantification of Dehalococcoides (Dhc) and specific reductive dechlorination (RD) gene population, and hydrochemical data in microcosm experiments and field samples was applied. Despite the high partitioning of CEs into the EVO phase, the carbon isotopic values of the remaining CEs fraction in the aqueous phase did not exhibit significant changes caused by phase partitioning in laboratory experiments. Both microcosm experiments and field data revealed a rapid RD of PCE and TCE, resulting in the transient accumulation of cis-DCE, which was slowly degraded to vinyl chloride (VC). These results agreed with the presence of Dhc populations and a shift to stronger reducing conditions in the field: i) RD functional genes (tceA, vcrA and bvcA) exhibited a trend to higher values and ii) a substantial increase in Dhc populations (up to 30% of the total bacterial populations) was observed over time. The dual-element isotope slope ΛC-Cl for RD of cis-DCE obtained from field data (ΛC – Cl = 5 ± 3) was similar to the one determined from the microcosm experiments under controlled anoxic conditions (ΛC – Cl = 4.9 ± 0.8). However, ΛC-Cl values differ from those reported so far for laboratory studies with Dhc strains and mixed cultures containing Dhc, i.e., between 8.3 and 17.8. This observation underscores the potential variety of reductive dehalogenases involved during cis-DCE RD and the importance of determining site-specific Λ and ε values in order to improve the identification and quantification of transformation processes in the field.Elsevier B.V.2025202520242025info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion12 p.application/pdfhttps://hdl.handle.net/2445/218415Articles publicats en revistes (Mineralogia, Petrologia i Geologia Aplicada)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1016/j.scitotenv.2024.175351Science of the Total Environment, 2024, vol. 951, 175351cc-by (c) The Authors., 2024http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2184152026-05-29T05:05:01Z
dc.title.none.fl_str_mv	Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO)
title	Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO)
spellingShingle	Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO) Gil-Villalba, Sergio Bioremediació Depuració d'aigües residuals Isòtops Contaminació de l'aigua Bioremediation Purification of sewage Isotopes Water pollution
title_short	Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO)
title_full	Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO)
title_fullStr	Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO)
title_full_unstemmed	Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO)
title_sort	Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO)
dc.creator.none.fl_str_mv	Gil-Villalba, Sergio Palau, Jordi Soder-Walz, Jesica M. Vallecillo, Miguel A. Corregidor, Jordi Tirado, Andrea Shouakar-Stash, O. Guivernau, Miriam Viñas, Miquel Soler, Anna Rosell, Mònica
author	Gil-Villalba, Sergio
author_facet	Gil-Villalba, Sergio Palau, Jordi Soder-Walz, Jesica M. Vallecillo, Miguel A. Corregidor, Jordi Tirado, Andrea Shouakar-Stash, O. Guivernau, Miriam Viñas, Miquel Soler, Anna Rosell, Mònica
author_role	author
author2	Palau, Jordi Soder-Walz, Jesica M. Vallecillo, Miguel A. Corregidor, Jordi Tirado, Andrea Shouakar-Stash, O. Guivernau, Miriam Viñas, Miquel Soler, Anna Rosell, Mònica
author2_role	author author author author author author author author author author
dc.subject.none.fl_str_mv	Bioremediació Depuració d'aigües residuals Isòtops Contaminació de l'aigua Bioremediation Purification of sewage Isotopes Water pollution
topic	Bioremediació Depuració d'aigües residuals Isòtops Contaminació de l'aigua Bioremediation Purification of sewage Isotopes Water pollution
description	Enhanced In Situ Bioremediation (EISB) using Emulsified Vegetable Oil (EVO) as a long-term electron donor has gained prominence for the treatment of groundwater contaminated with chlorinated ethenes (CEs). This study explores the potential of isotopic and molecular biology tools (MBT) to investigate the CEs (PCE, TCE and cis-DCE) bioremediation using EVO in a contaminated site. A multiple approach using C and Cl-CSIA, quantification of Dehalococcoides (Dhc) and specific reductive dechlorination (RD) gene population, and hydrochemical data in microcosm experiments and field samples was applied. Despite the high partitioning of CEs into the EVO phase, the carbon isotopic values of the remaining CEs fraction in the aqueous phase did not exhibit significant changes caused by phase partitioning in laboratory experiments. Both microcosm experiments and field data revealed a rapid RD of PCE and TCE, resulting in the transient accumulation of cis-DCE, which was slowly degraded to vinyl chloride (VC). These results agreed with the presence of Dhc populations and a shift to stronger reducing conditions in the field: i) RD functional genes (tceA, vcrA and bvcA) exhibited a trend to higher values and ii) a substantial increase in Dhc populations (up to 30% of the total bacterial populations) was observed over time. The dual-element isotope slope ΛC-Cl for RD of cis-DCE obtained from field data (ΛC – Cl = 5 ± 3) was similar to the one determined from the microcosm experiments under controlled anoxic conditions (ΛC – Cl = 4.9 ± 0.8). However, ΛC-Cl values differ from those reported so far for laboratory studies with Dhc strains and mixed cultures containing Dhc, i.e., between 8.3 and 17.8. This observation underscores the potential variety of reductive dehalogenases involved during cis-DCE RD and the importance of determining site-specific Λ and ε values in order to improve the identification and quantification of transformation processes in the field.
publishDate	2024
dc.date.none.fl_str_mv	2024 2025 2025 2025
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion
format	article
status_str	acceptedVersion
dc.identifier.none.fl_str_mv	https://hdl.handle.net/2445/218415
url	https://hdl.handle.net/2445/218415
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	Reproducció del document publicat a: https://doi.org/10.1016/j.scitotenv.2024.175351 Science of the Total Environment, 2024, vol. 951, 175351
dc.rights.none.fl_str_mv	cc-by (c) The Authors., 2024 http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess
rights_invalid_str_mv	cc-by (c) The Authors., 2024 http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	12 p. application/pdf
dc.publisher.none.fl_str_mv	Elsevier B.V.
publisher.none.fl_str_mv	Elsevier B.V.
dc.source.none.fl_str_mv	Articles publicats en revistes (Mineralogia, Petrologia i Geologia Aplicada) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str	Recercat. Dipósit de la Recerca de Catalunya
collection	Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO)

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