Linking the effect of antibiotics on partial-nitritation biofilters: Performance, microbial communities and microbial activities

The emergence and spread of antibiotics resistance in wastewater treatment systems have been pointed as a major environmental health problem. Nevertheless, research about adaptation and antibiotics resistance gain in wastewater treatment systems subjected to antibiotics has not been successfully dev...

Full description

Bibliographic Details
Authors: González-Martínez, Alejandro, Margareto, Alejandro, Rodríguez-Sánchez, Alejandro, Pesciaroli, Chiara, Diaz-Cruz, Silvia, Barceló, Damià, Vahala, Riku
Format: article
Status:Published version
Publication Date:2018
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/174073
Online Access:http://hdl.handle.net/10261/174073
Access Level:Open access
Keyword:Antibiotic resistance
Autotrophic nitrogen removal
Metatranscriptomics
Microbial activity
Microbial population
Partial-nitritation
id ES_ee37be987431c0830a40e1df4f0eecda
oai_identifier_str oai:digital.csic.es:10261/174073
network_acronym_str ES
network_name_str España
repository_id_str
spelling Linking the effect of antibiotics on partial-nitritation biofilters: Performance, microbial communities and microbial activitiesGonzález-Martínez, AlejandroMargareto, AlejandroRodríguez-Sánchez, AlejandroPesciaroli, ChiaraDiaz-Cruz, SilviaBarceló, DamiàVahala, RikuAntibiotic resistanceAutotrophic nitrogen removalMetatranscriptomicsMicrobial activityMicrobial populationPartial-nitritationThe emergence and spread of antibiotics resistance in wastewater treatment systems have been pointed as a major environmental health problem. Nevertheless, research about adaptation and antibiotics resistance gain in wastewater treatment systems subjected to antibiotics has not been successfully developed considering bioreactor performance, microbial community dynamics and microbial activity dynamics at the same time. To observe this in autotrophic nitrogen removal systems, a partial-nitritation biofilter was subjected to a continuous loading of antibiotics mix of azithromycin, norfloxacin, trimethoprim, and sulfamethoxazole. The effect of the antibiotics mix over the performance, bacterial communities and bacterial activity in the system was evaluated. The addition of antibiotics caused a drop of ammonium oxidation efficiency (from 50 to 5%) and of biomass concentration in the bioreactor, which was coupled to the loss of ammonium oxidizing bacteria Nitrosomonas in the bacterial community from 40 to 3%. Biomass in the partial nitritation biofilter experienced a sharp decrease of about 80% due to antibiotics loading, but the biomass adapted and experienced a growth by stabilization under antibiotics feeding. During the experiment several bacterial genera appeared, such as Alcaligenes, Paracoccus, and Acidovorax, clearly dominating the bacterial community with > 20% relative abundance. The system reached around 30% ammonium oxidation efficiency after adaptation to antibiotics, but no effluent nitrite was found, suggesting that dominant antibiotics-resistant phylotypes could be involved in nitrification-denitrification metabolisms. The activity of ammonium oxidation measured as amoA and hao gene expression dropped a 98.25% and 99.21%, respectively, comparing the system before and after the addition of antibiotics. On the other hand, denitrifying activity increased as observed by higher expression of nir and nos genes (83.14% and 252.54%, respectively). In addition, heterotrophic nitrification cyt c-551 was active only after the antibiotics addition. Resistance to the antibiotics was presumably given by ermF, carA and msrA for azithromycin, mutations of the gyrA and grlB for norfloxacin, and by sul123 genes for sulfamethoxazole. Joined physicochemical and microbiological characterization of the system were used to investigate the effect of the antibiotics over the bioprocess. Despite the antibiotics resistance, activity of Bacteria decreased while the activity of Archaea and Fungi increased. © 2018 Gonzalez-Martinez, Margareto, Rodriguez-Sanchez, Pesciaroli, Diaz-Cruz, Barcelo and Vahala.The authors would like to acknowledge the support given by the Built Environment Department in the Aalto University and the Institute of Water Research of the University of Granada. They also acknowledge the research funding from the Generalitat de Catalunya (Consolidated Research Group: Water and Soil Quality Unit 2014-SGR-418). Finally, they want to give thanks to B. San Miguel-Conejero for her microbiological support.Peer reviewedFrontiers MediaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/174073reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3389/fmicb.2018.00354Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1740732026-05-22T06:33:51Z
dc.title.none.fl_str_mv Linking the effect of antibiotics on partial-nitritation biofilters: Performance, microbial communities and microbial activities
title Linking the effect of antibiotics on partial-nitritation biofilters: Performance, microbial communities and microbial activities
spellingShingle Linking the effect of antibiotics on partial-nitritation biofilters: Performance, microbial communities and microbial activities
González-Martínez, Alejandro
Antibiotic resistance
Autotrophic nitrogen removal
Metatranscriptomics
Microbial activity
Microbial population
Partial-nitritation
title_short Linking the effect of antibiotics on partial-nitritation biofilters: Performance, microbial communities and microbial activities
title_full Linking the effect of antibiotics on partial-nitritation biofilters: Performance, microbial communities and microbial activities
title_fullStr Linking the effect of antibiotics on partial-nitritation biofilters: Performance, microbial communities and microbial activities
title_full_unstemmed Linking the effect of antibiotics on partial-nitritation biofilters: Performance, microbial communities and microbial activities
title_sort Linking the effect of antibiotics on partial-nitritation biofilters: Performance, microbial communities and microbial activities
dc.creator.none.fl_str_mv González-Martínez, Alejandro
Margareto, Alejandro
Rodríguez-Sánchez, Alejandro
Pesciaroli, Chiara
Diaz-Cruz, Silvia
Barceló, Damià
Vahala, Riku
author González-Martínez, Alejandro
author_facet González-Martínez, Alejandro
Margareto, Alejandro
Rodríguez-Sánchez, Alejandro
Pesciaroli, Chiara
Diaz-Cruz, Silvia
Barceló, Damià
Vahala, Riku
author_role author
author2 Margareto, Alejandro
Rodríguez-Sánchez, Alejandro
Pesciaroli, Chiara
Diaz-Cruz, Silvia
Barceló, Damià
Vahala, Riku
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Antibiotic resistance
Autotrophic nitrogen removal
Metatranscriptomics
Microbial activity
Microbial population
Partial-nitritation
topic Antibiotic resistance
Autotrophic nitrogen removal
Metatranscriptomics
Microbial activity
Microbial population
Partial-nitritation
description The emergence and spread of antibiotics resistance in wastewater treatment systems have been pointed as a major environmental health problem. Nevertheless, research about adaptation and antibiotics resistance gain in wastewater treatment systems subjected to antibiotics has not been successfully developed considering bioreactor performance, microbial community dynamics and microbial activity dynamics at the same time. To observe this in autotrophic nitrogen removal systems, a partial-nitritation biofilter was subjected to a continuous loading of antibiotics mix of azithromycin, norfloxacin, trimethoprim, and sulfamethoxazole. The effect of the antibiotics mix over the performance, bacterial communities and bacterial activity in the system was evaluated. The addition of antibiotics caused a drop of ammonium oxidation efficiency (from 50 to 5%) and of biomass concentration in the bioreactor, which was coupled to the loss of ammonium oxidizing bacteria Nitrosomonas in the bacterial community from 40 to 3%. Biomass in the partial nitritation biofilter experienced a sharp decrease of about 80% due to antibiotics loading, but the biomass adapted and experienced a growth by stabilization under antibiotics feeding. During the experiment several bacterial genera appeared, such as Alcaligenes, Paracoccus, and Acidovorax, clearly dominating the bacterial community with > 20% relative abundance. The system reached around 30% ammonium oxidation efficiency after adaptation to antibiotics, but no effluent nitrite was found, suggesting that dominant antibiotics-resistant phylotypes could be involved in nitrification-denitrification metabolisms. The activity of ammonium oxidation measured as amoA and hao gene expression dropped a 98.25% and 99.21%, respectively, comparing the system before and after the addition of antibiotics. On the other hand, denitrifying activity increased as observed by higher expression of nir and nos genes (83.14% and 252.54%, respectively). In addition, heterotrophic nitrification cyt c-551 was active only after the antibiotics addition. Resistance to the antibiotics was presumably given by ermF, carA and msrA for azithromycin, mutations of the gyrA and grlB for norfloxacin, and by sul123 genes for sulfamethoxazole. Joined physicochemical and microbiological characterization of the system were used to investigate the effect of the antibiotics over the bioprocess. Despite the antibiotics resistance, activity of Bacteria decreased while the activity of Archaea and Fungi increased. © 2018 Gonzalez-Martinez, Margareto, Rodriguez-Sanchez, Pesciaroli, Diaz-Cruz, Barcelo and Vahala.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/174073
url http://hdl.handle.net/10261/174073
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3389/fmicb.2018.00354
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869423625293004801
score 15,811543

Similar Items