Type I and Type II photosensitization of DNA etheno adducts

[EN] Photophysical and photochemical studies were carried out to examine the photoreactivity of etheno adducts, 1,N6-ethenoadenine (epsilon dA) and 1,N2-ethenoguanine (epsilon dG), in the presence of two well-known photosensitizers acting by Type I and/or Type II mechanisms such as 4-carboxybenzophe...

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Autores: Lizondo-Aranda, Paloma, Miranda, Miguel A., Heyne, Belinda, Rodríguez Muñiz, Gemma María|||0000-0001-8989-2401, Lhiaubet, Virginie Lyria|||0000-0002-8205-8892
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/220226
Acceso en línea:https://riunet.upv.es/handle/10251/220226
Access Level:acceso abierto
Palabra clave:Singlet oxygen
DNA lesions
Electron transfer
Lipid peroxidation
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spelling Type I and Type II photosensitization of DNA etheno adductsLizondo-Aranda, PalomaMiranda, Miguel A.Heyne, BelindaRodríguez Muñiz, Gemma María|||0000-0001-8989-2401Lhiaubet, Virginie Lyria|||0000-0002-8205-8892Singlet oxygenDNA lesionsElectron transferLipid peroxidation[EN] Photophysical and photochemical studies were carried out to examine the photoreactivity of etheno adducts, 1,N6-ethenoadenine (epsilon dA) and 1,N2-ethenoguanine (epsilon dG), in the presence of two well-known photosensitizers acting by Type I and/or Type II mechanisms such as 4-carboxybenzophenone (CBP) and rose Bengal (RB), respectively. Steady-state photolysis experiments combined with HPLC and mass spectroscopy measurements lead to photoproducts that correspond to the repaired nucleosides. To determine the mechanism of this photooxidation processes, phosphorescence spectroscopy, direct detection of singlet oxygen luminescence and laser flash photolysis were carried out. This work establishes that epsilon dG and epsilon dA are sensitive to both types of processes (Type I and II).Financial support from the Spanish (projects PID2021-128348NB-I00 and Severo Ochoa Center of Excellence Program CEX2021-001230-S, funded by MCIN/AEI/https://doi.org/10.13039/501100011033/and "FEDER a way of making Europe", and grant BES-2016-077517 to P. L.-A.) and regional (CIAICO/2021/061) governments is acknowledged.The Royal Society of ChemistryInstituto Universitario Mixto de Tecnología QuímicaAgencia Estatal de InvestigaciónEuropean Regional Development FundMinisterio de Ciencia e InnovaciónUniversitat Politècnica de ValènciaConselleria de Innovación, Universidades, Ciencia y Sociedad Digital, Generalitat ValencianaRepositorio Institucional de la Universitat Politècnica de València Riunet20252025-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/220226reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2021-128348NB-I00 TRANSFERENCIA DE ENERGIA TRIPLETE-TRIPLETE A LARGA DISTANCIA EN SISTEMAS MODELO DE ADNGeneralitat Valenciana https://doi.org/10.13039/501100003359 CIAICO%2F2021%2F061 Sunscreen-based photocagesAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 CEX2021-001230-SMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BES-2016-077517 Lesiones del ADN como fotosensibilizadores intrínsecos El concepto de caballo de Troyaopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2202262026-06-13T07:49:27Z
dc.title.none.fl_str_mv Type I and Type II photosensitization of DNA etheno adducts
title Type I and Type II photosensitization of DNA etheno adducts
spellingShingle Type I and Type II photosensitization of DNA etheno adducts
Lizondo-Aranda, Paloma
Singlet oxygen
DNA lesions
Electron transfer
Lipid peroxidation
title_short Type I and Type II photosensitization of DNA etheno adducts
title_full Type I and Type II photosensitization of DNA etheno adducts
title_fullStr Type I and Type II photosensitization of DNA etheno adducts
title_full_unstemmed Type I and Type II photosensitization of DNA etheno adducts
title_sort Type I and Type II photosensitization of DNA etheno adducts
dc.creator.none.fl_str_mv Lizondo-Aranda, Paloma
Miranda, Miguel A.
Heyne, Belinda
Rodríguez Muñiz, Gemma María|||0000-0001-8989-2401
Lhiaubet, Virginie Lyria|||0000-0002-8205-8892
author Lizondo-Aranda, Paloma
author_facet Lizondo-Aranda, Paloma
Miranda, Miguel A.
Heyne, Belinda
Rodríguez Muñiz, Gemma María|||0000-0001-8989-2401
Lhiaubet, Virginie Lyria|||0000-0002-8205-8892
author_role author
author2 Miranda, Miguel A.
Heyne, Belinda
Rodríguez Muñiz, Gemma María|||0000-0001-8989-2401
Lhiaubet, Virginie Lyria|||0000-0002-8205-8892
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Tecnología Química
Agencia Estatal de Investigación
European Regional Development Fund
Ministerio de Ciencia e Innovación
Universitat Politècnica de València
Conselleria de Innovación, Universidades, Ciencia y Sociedad Digital, Generalitat Valenciana
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Singlet oxygen
DNA lesions
Electron transfer
Lipid peroxidation
topic Singlet oxygen
DNA lesions
Electron transfer
Lipid peroxidation
description [EN] Photophysical and photochemical studies were carried out to examine the photoreactivity of etheno adducts, 1,N6-ethenoadenine (epsilon dA) and 1,N2-ethenoguanine (epsilon dG), in the presence of two well-known photosensitizers acting by Type I and/or Type II mechanisms such as 4-carboxybenzophenone (CBP) and rose Bengal (RB), respectively. Steady-state photolysis experiments combined with HPLC and mass spectroscopy measurements lead to photoproducts that correspond to the repaired nucleosides. To determine the mechanism of this photooxidation processes, phosphorescence spectroscopy, direct detection of singlet oxygen luminescence and laser flash photolysis were carried out. This work establishes that epsilon dG and epsilon dA are sensitive to both types of processes (Type I and II).
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/220226
url https://riunet.upv.es/handle/10251/220226
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2021-128348NB-I00 TRANSFERENCIA DE ENERGIA TRIPLETE-TRIPLETE A LARGA DISTANCIA EN SISTEMAS MODELO DE ADN
Generalitat Valenciana https://doi.org/10.13039/501100003359 CIAICO%2F2021%2F061 Sunscreen-based photocages
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 CEX2021-001230-S
Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BES-2016-077517 Lesiones del ADN como fotosensibilizadores intrínsecos El concepto de caballo de Troya
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Royal Society of Chemistry
publisher.none.fl_str_mv The Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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