Tracking the emergence and dissemination of a blaNDM-23 gene in a multidrug resistance plasmid of Klebsiella pneumoniae

On behalf of the Networked Laboratory for Antimicrobial Resistance Surveillance of Comunitat Valenciana (Spain).

Detalles Bibliográficos
Autores: García-González, Neris, Beamud, Beatriz, Fuster Escrivá, Begoña, Giner, Salvador, Domínguez, Maria Victoria, Sánchez, Antonia, Sevilla, Jordi, Coque, Teresa, Gimeno-Cardona, Concepción, González-Candelas, Fernando
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/353413
Acceso en línea:http://hdl.handle.net/10261/353413
Access Level:acceso abierto
Palabra clave:Klebsiella pneumoniae
MDR plasmid
Novel NDM
Genomic epidemiology
Complex class 1 integron
XDR plasmid
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network_name_str España
repository_id_str
dc.title.none.fl_str_mv Tracking the emergence and dissemination of a blaNDM-23 gene in a multidrug resistance plasmid of Klebsiella pneumoniae
title Tracking the emergence and dissemination of a blaNDM-23 gene in a multidrug resistance plasmid of Klebsiella pneumoniae
spellingShingle Tracking the emergence and dissemination of a blaNDM-23 gene in a multidrug resistance plasmid of Klebsiella pneumoniae
García-González, Neris
Klebsiella pneumoniae
MDR plasmid
Novel NDM
Genomic epidemiology
Complex class 1 integron
XDR plasmid
title_short Tracking the emergence and dissemination of a blaNDM-23 gene in a multidrug resistance plasmid of Klebsiella pneumoniae
title_full Tracking the emergence and dissemination of a blaNDM-23 gene in a multidrug resistance plasmid of Klebsiella pneumoniae
title_fullStr Tracking the emergence and dissemination of a blaNDM-23 gene in a multidrug resistance plasmid of Klebsiella pneumoniae
title_full_unstemmed Tracking the emergence and dissemination of a blaNDM-23 gene in a multidrug resistance plasmid of Klebsiella pneumoniae
title_sort Tracking the emergence and dissemination of a blaNDM-23 gene in a multidrug resistance plasmid of Klebsiella pneumoniae
dc.creator.none.fl_str_mv García-González, Neris
Beamud, Beatriz
Fuster Escrivá, Begoña
Giner, Salvador
Domínguez, Maria Victoria
Sánchez, Antonia
Sevilla, Jordi
Coque, Teresa
Gimeno-Cardona, Concepción
González-Candelas, Fernando
author García-González, Neris
author_facet García-González, Neris
Beamud, Beatriz
Fuster Escrivá, Begoña
Giner, Salvador
Domínguez, Maria Victoria
Sánchez, Antonia
Sevilla, Jordi
Coque, Teresa
Gimeno-Cardona, Concepción
González-Candelas, Fernando
author_role author
author2 Beamud, Beatriz
Fuster Escrivá, Begoña
Giner, Salvador
Domínguez, Maria Victoria
Sánchez, Antonia
Sevilla, Jordi
Coque, Teresa
Gimeno-Cardona, Concepción
González-Candelas, Fernando
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Ministerio de Ciencia e Innovación (España)
European Commission
Agencia Estatal de Investigación (España)
Generalitat Valenciana
Instituto de Salud Carlos III
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Klebsiella pneumoniae
MDR plasmid
Novel NDM
Genomic epidemiology
Complex class 1 integron
XDR plasmid
topic Klebsiella pneumoniae
MDR plasmid
Novel NDM
Genomic epidemiology
Complex class 1 integron
XDR plasmid
description On behalf of the Networked Laboratory for Antimicrobial Resistance Surveillance of Comunitat Valenciana (Spain).
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/353413
url http://hdl.handle.net/10261/353413
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2017-89594-R
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1128/spectrum.02585-22
https://doi.org/10.1128/spectrum.02585-22
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869423436056494080
spelling Tracking the emergence and dissemination of a blaNDM-23 gene in a multidrug resistance plasmid of Klebsiella pneumoniaeGarcía-González, NerisBeamud, BeatrizFuster Escrivá, BegoñaGiner, SalvadorDomínguez, Maria VictoriaSánchez, AntoniaSevilla, JordiCoque, TeresaGimeno-Cardona, ConcepciónGonzález-Candelas, FernandoKlebsiella pneumoniaeMDR plasmidNovel NDMGenomic epidemiologyComplex class 1 integronXDR plasmidOn behalf of the Networked Laboratory for Antimicrobial Resistance Surveillance of Comunitat Valenciana (Spain).Since the discovery of blaNDM-1, NDM β-lactamases have become one of the most widespread carbapenemases worldwide. To date, 43 different NDM variants have been reported but some, such as blaNDM-23, have not been characterized in detail yet. Here, we describe the emergence of a novel blaNDM-23 allele from a blaNDM-1 ancestor and the multidrug resistance plasmid that has disseminated it through a Klebsiella pneumoniae ST437 clone in several Spanish hospitals. Between 2016 and 2019, 1,972 isolates were collected in an epidemiological survey for extended-spectrum-β-lactamase (ESBL)-producing Klebsiella pneumoniae in the Comunitat Valenciana (Spain). Three carbapenem-resistant strains failed to be detected by carbapenemase-producing Enterobacteriaceae (CPE) screening tests. These isolates carried a blaNDM-23 gene. To characterize this gene, its emergence, and its dissemination, we performed antimicrobial susceptibility tests, hybrid sequencing with Illumina and Nanopore technologies, and phylogenetic analyses. The MICs of the blaNDM-23 allele were identical to those of the blaNDM-1 allele. The blaNDM-23 allele was found in 14 isolates on a 97-kb nonmobilizable, multidrug-resistant plasmid carrying 19 resistance genes for 9 different antimicrobial families. In this plasmid, the blaNDM-23 gene is in the variable region of a complex class 1 integron with a singular genetic environment. The small genetic distance between blaNDM-23-producing isolates reflects a 5-year-long clonal dispersion involving several hospitals and interregional spread. We have characterized the genomic and epidemiological contexts in the emergence and community spread of a new blaNDM-23 allele in a multidrug resistance (MDR) plasmid of Klebsiella pneumoniae.[Importance] At a time when antimicrobial resistance has become one of the biggest concerns worldwide, the emergence of novel alleles and extremely drug-resistant plasmids is a threat to public health worldwide, especially when they produce carbapenem resistance in one of the most problematic pathogens, such as Klebsiella pneumoniae. We used genomic epidemiology to describe the emergence of a novel NDM-23 allele and identify it in a MDR plasmid that has disseminated through a K. pneumoniae ST437 clone in several hospitals in Spain. Using bioinformatic and phylogenetic analyses, we have traced the evolutionary and epidemiological route of the new allele, the hosting plasmid, and the strain that carried both of them from Pakistan to Spain. A better understanding of the NDM-producing K. pneumoniae populations and plasmids has made evident the spread of this clone through the region, enhancing the importance of genomic surveillance in the control of antimicrobial resistance.This research was supported by project BFU2017-89594R (MICIN, Spanish Government) and by the European Union through the Operational Program of European Regional Development Fund (ERDF) of Valencia Region (Spain) 2014-2020. Additional support was provided by the Network Research Centre for Epidemiology and Public Health (CIBERESP).Peer reviewedAmerican Society for MicrobiologyMinisterio de Ciencia, Innovación y Universidades (España)Ministerio de Ciencia e Innovación (España)European CommissionAgencia Estatal de Investigación (España)Generalitat ValencianaInstituto de Salud Carlos IIIConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/353413reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2017-89594-RThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1128/spectrum.02585-22https://doi.org/10.1128/spectrum.02585-22Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3534132026-05-22T06:33:51Z
score 15,811543

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