The Addiction-Related Protein ANKK1 is Differentially Expressed During the Cell Cycle in Neural Precursors

TaqIA is a polymorphism associated with addictions and dopamine-related traits. It is located in the ankyrin repeat and kinase domain containing 1 gene (ANKK1) nearby the gene for the dopamine D2 receptor (D2R). Since ANKK1 function is unknown, TaqIA-associated traits have been explained only by dif...

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Autores: España-Serrano L, Guerra Martín-Palanco N, Montero-Pedrazuela A, Pérez-Santamarina E, Vidal R, García-Consuegra I, Valdizán EM, Pazos A, Palomo T, Jiménez-Arriero MÁ, Guadaño-Ferraz A, Hoenicka J
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Recursos:Fundació Sant Joan de Déu
Repositorio:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
OAI Identifier:oai:fsjd.fundanetsuite.com:p9855
Acesso em linha:https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=9855
Access Level:acceso abierto
Palavra-chave:addictions
ANKK1
cell cycle
DRD2
neurogenesis
TaqIA
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repository_id_str
spelling The Addiction-Related Protein ANKK1 is Differentially Expressed During the Cell Cycle in Neural PrecursorsEspaña-Serrano LGuerra Martín-Palanco NMontero-Pedrazuela APérez-Santamarina EVidal RGarcía-Consuegra IValdizán EMPazos APalomo TJiménez-Arriero MÁGuadaño-Ferraz AHoenicka JaddictionsANKK1cell cycleDRD2neurogenesisTaqIATaqIA is a polymorphism associated with addictions and dopamine-related traits. It is located in the ankyrin repeat and kinase domain containing 1 gene (ANKK1) nearby the gene for the dopamine D2 receptor (D2R). Since ANKK1 function is unknown, TaqIA-associated traits have been explained only by differences in D2R. Here we report ANKK1 studies in mouse and human brain using quantitative real-time PCR, Western blot, immunohistochemistry, and flow cytometry. ANKK1 mRNA and protein isoforms vary along neurodevelopment in the human and mouse brain. In mouse adult brain ANKK1 is located in astrocytes, nuclei of postmitotic neurons and neural precursors from neurogenic niches. In both embryos and adults, nuclei of neural precursors show significant variation of ANKK1 intensity. We demonstrate a correlation between ANKK1 and the cell cycle. Cell synchronization experiments showed a significant increment of ANKK1-kinase in mitotic cells while ANKK1-kinase overexpression affects G1 and M phase that were found to be modulated by ANKK1 alleles and apomorphine treatment. Furthermore, during embryonic neurogenesis ANKK1 was expressed in slow-dividing neuroblasts and rapidly dividing precursors which are mitotic cells. These results suggest a role of ANKK1 during the cell cycle in neural precursors thus providing biological support to brain structure involvement in the TaqIA-associated phenotypes.OXFORD UNIV PRESS INC2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=9855CEREBRAL CORTEXISSN: 10473211ISSNe: 14602199reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déuinstname:Fundació Sant Joan de DéuInglésinfo:eu-repo/semantics/openAccessoai:fsjd.fundanetsuite.com:p98552026-05-27T12:37:41Z
dc.title.none.fl_str_mv The Addiction-Related Protein ANKK1 is Differentially Expressed During the Cell Cycle in Neural Precursors
title The Addiction-Related Protein ANKK1 is Differentially Expressed During the Cell Cycle in Neural Precursors
spellingShingle The Addiction-Related Protein ANKK1 is Differentially Expressed During the Cell Cycle in Neural Precursors
España-Serrano L
addictions
ANKK1
cell cycle
DRD2
neurogenesis
TaqIA
title_short The Addiction-Related Protein ANKK1 is Differentially Expressed During the Cell Cycle in Neural Precursors
title_full The Addiction-Related Protein ANKK1 is Differentially Expressed During the Cell Cycle in Neural Precursors
title_fullStr The Addiction-Related Protein ANKK1 is Differentially Expressed During the Cell Cycle in Neural Precursors
title_full_unstemmed The Addiction-Related Protein ANKK1 is Differentially Expressed During the Cell Cycle in Neural Precursors
title_sort The Addiction-Related Protein ANKK1 is Differentially Expressed During the Cell Cycle in Neural Precursors
dc.creator.none.fl_str_mv España-Serrano L
Guerra Martín-Palanco N
Montero-Pedrazuela A
Pérez-Santamarina E
Vidal R
García-Consuegra I
Valdizán EM
Pazos A
Palomo T
Jiménez-Arriero MÁ
Guadaño-Ferraz A
Hoenicka J
author España-Serrano L
author_facet España-Serrano L
Guerra Martín-Palanco N
Montero-Pedrazuela A
Pérez-Santamarina E
Vidal R
García-Consuegra I
Valdizán EM
Pazos A
Palomo T
Jiménez-Arriero MÁ
Guadaño-Ferraz A
Hoenicka J
author_role author
author2 Guerra Martín-Palanco N
Montero-Pedrazuela A
Pérez-Santamarina E
Vidal R
García-Consuegra I
Valdizán EM
Pazos A
Palomo T
Jiménez-Arriero MÁ
Guadaño-Ferraz A
Hoenicka J
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv addictions
ANKK1
cell cycle
DRD2
neurogenesis
TaqIA
topic addictions
ANKK1
cell cycle
DRD2
neurogenesis
TaqIA
description TaqIA is a polymorphism associated with addictions and dopamine-related traits. It is located in the ankyrin repeat and kinase domain containing 1 gene (ANKK1) nearby the gene for the dopamine D2 receptor (D2R). Since ANKK1 function is unknown, TaqIA-associated traits have been explained only by differences in D2R. Here we report ANKK1 studies in mouse and human brain using quantitative real-time PCR, Western blot, immunohistochemistry, and flow cytometry. ANKK1 mRNA and protein isoforms vary along neurodevelopment in the human and mouse brain. In mouse adult brain ANKK1 is located in astrocytes, nuclei of postmitotic neurons and neural precursors from neurogenic niches. In both embryos and adults, nuclei of neural precursors show significant variation of ANKK1 intensity. We demonstrate a correlation between ANKK1 and the cell cycle. Cell synchronization experiments showed a significant increment of ANKK1-kinase in mitotic cells while ANKK1-kinase overexpression affects G1 and M phase that were found to be modulated by ANKK1 alleles and apomorphine treatment. Furthermore, during embryonic neurogenesis ANKK1 was expressed in slow-dividing neuroblasts and rapidly dividing precursors which are mitotic cells. These results suggest a role of ANKK1 during the cell cycle in neural precursors thus providing biological support to brain structure involvement in the TaqIA-associated phenotypes.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=9855
url https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=9855
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv OXFORD UNIV PRESS INC
publisher.none.fl_str_mv OXFORD UNIV PRESS INC
dc.source.none.fl_str_mv CEREBRAL CORTEX
ISSN: 10473211
ISSNe: 14602199
reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
instname:Fundació Sant Joan de Déu
instname_str Fundació Sant Joan de Déu
reponame_str r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
collection r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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