ANKK1 Is a Wnt/PCP Scaffold Protein for Neural F-ACTIN Assembly.

The IA polymorphism is a marker of both the Ankyrin Repeat and Kinase Domain containing I gene () encoding a RIP-kinase, and the gene for the dopamine receptor D2. Despite a large number of studies of IA in addictions and other psychiatric disorders, there is difficulty in interpreting this genetic...

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Detalhes bibliográficos
Autores: Domínguez-Berzosa, Laura, Cantarero, Lara, Rodríguez-Sanz, María, Tort, Gemma, Garrido, Elena, Troya-Balseca, Johanna, Sáez, María, Castro-Martínez, Xóchitl Helga, Fernandez-Lizarbe, Sara, Urquizu, Edurne, Calvo, Enrique, López, Juan Antonio, Palomo, Tomás, Palau, Francesc, Hoenicka, Janet
Formato: artículo
Fecha de publicación:2024
País:España
Recursos:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/25910
Acesso em linha:https://hdl.handle.net/20.500.12105/25910
Access Level:acceso abierto
Palavra-chave:ANKK1
F-ACTIN
FARP1
TaqIA
Wnt/PCP
addictions
Descrição
Resumo:The IA polymorphism is a marker of both the Ankyrin Repeat and Kinase Domain containing I gene () encoding a RIP-kinase, and the gene for the dopamine receptor D2. Despite a large number of studies of IA in addictions and other psychiatric disorders, there is difficulty in interpreting this genetic phenomenon due to the lack of knowledge about ANKK1 function. In SH-SY5Y neuroblastoma models, we show that ANKK1 interacts with the synapse protein FERM ARH/RhoGEF and Pleckstrin Domain 1 (FARP1), which is a guanine nucleotide exchange factor (GEF) of the RhoGTPases RAC1 and RhoA. ANKK1-FARP1 colocalized in F-ACTIN-rich structures for neuronal maturation and migration, and both proteins activate the Wnt/PCP pathway. ANKK1, but not FARP1, promotes neuritogenesis, and both proteins are involved in neuritic spine outgrowth. Notably, the knockdown of or affects RhoGTPases expression and neural differentiation. Additionally, ANKK1 binds WGEF, another GEF of Wnt/PCP, regulating its interaction with RhoA. During neuronal differentiation, ANKK1-WGEF interaction is downregulated, while ANKK1-FARP1 interaction is increased, suggesting that ANKK1 recruits Wnt/PCP components for bidirectional control of F-ACTIN assembly. Our results suggest a brain structural basis in IA-associated phenotypes.

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