Cost-effective laser diode scanning 3D photoacoustic microscopy of melanocytic dermal tumors in situ
Management of melanoma skin cancer involves a wide excision followed by histopathological analysis to confirm the diagnosis, which is only guided by superficial dermoscopy. Dermatological oncology can thus benefit from high-resolution, 3D images provided by photoacoustic microscopy; however, its cli...
| Autores: | , , , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2026 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/419175 |
| Acesso em linha: | http://hdl.handle.net/10261/419175 https://api.elsevier.com/content/abstract/scopus_id/105017230179 |
| Access Level: | acceso abierto |
| Palavra-chave: | Cost-effective 3D photoacoustic microscopy Laser diode scanning Melanoma imaging Point of care |
| Resumo: | Management of melanoma skin cancer involves a wide excision followed by histopathological analysis to confirm the diagnosis, which is only guided by superficial dermoscopy. Dermatological oncology can thus benefit from high-resolution, 3D images provided by photoacoustic microscopy; however, its clinical translation is hindered by its elevated cost. Opening a pathway to point-of-care 3D melanoma assessment, we present the first demonstration of a cost-effective photoacoustic microscope (CePAM) utilizing fast laser diode scanning with a focused transducer in reflection mode. A novel application of a direct model inversion (DiMI) algorithm allowed a 55-fold enlargement of the image field of view beyond the transducer acoustic focus, reaching a 1-square millimeter laser-scanned area with uniform sensitivity (>20 dB). Furthermore, DiMI improved the intrinsic axial resolution of the system via multiple selective-plane scanning. The feasibility of CePAM is demonstrated by quantitative tests on phantoms and in vivo imaging of murine melanocytic dermal tumors, yielding accurate 3D images and virtual cross-sections consistent with histology. |
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