Stem cell-based gene delivery mediated by cationic niosomes for bone regeneration

Bone morphogenetic protein-7(BMP-7) plays a pivotal role in the transformation of mesenchymal stem cells (MSCs) into bone. However, its impact is hampered due to its short half-life. Therefore, gene therapy may be an interesting approach to deliver BMP-7 gene to D1-MSCs. In this manuscript we prepar...

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Detalles Bibliográficos
Autores: Attia, Noha, Mashal, Mohamed, Grijalvo, Santiago, Eritja Casadellà, Ramón, Zárate, Jon, Puras, Gustavo, Pedraz, José Luís
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/159063
Acceso en línea:http://hdl.handle.net/10261/159063
Access Level:acceso abierto
Palabra clave:Bone regeneration
Gene delivery
Niosomes
Stem cells
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spelling Stem cell-based gene delivery mediated by cationic niosomes for bone regenerationAttia, NohaMashal, MohamedGrijalvo, SantiagoEritja Casadellà, RamónZárate, JonPuras, GustavoPedraz, José LuísBone regenerationGene deliveryNiosomesStem cellsBone morphogenetic protein-7(BMP-7) plays a pivotal role in the transformation of mesenchymal stem cells (MSCs) into bone. However, its impact is hampered due to its short half-life. Therefore, gene therapy may be an interesting approach to deliver BMP-7 gene to D1-MSCs. In this manuscript we prepared and characterized niosomes based on cationic lipid 2,3-di(tetradecyloxy)propan-1-amine, combined with polysorbate 80 for gene delivery purposes. Niosomes were characterized and combined initially with pCMS-EGFP reporter plasmid, and later with pUNO1-hBMP-7 plasmid to evaluate osteogenesis differentiation. Additionally, specific blockers of most relevant endocytic pathways were used to evaluate the intracellular disposition of complexes. MSCs transfected with niosomes showed increased growth rate, enhanced alkaline phosphatase activity (ALP) and extracellular matrix deposition which suggested the formation of osteoblast-like cells. We concluded that hBMP-7-transfected MSCs could be considered not only as an effective delivery tool of hBMP-7, but also as proliferating and bone forming cells for bone regeneration.Peer reviewedElsevierConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201820182017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/159063reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés10.1016/j.nano.2017.11.005Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1590632026-05-22T06:33:51Z
dc.title.none.fl_str_mv Stem cell-based gene delivery mediated by cationic niosomes for bone regeneration
title Stem cell-based gene delivery mediated by cationic niosomes for bone regeneration
spellingShingle Stem cell-based gene delivery mediated by cationic niosomes for bone regeneration
Attia, Noha
Bone regeneration
Gene delivery
Niosomes
Stem cells
title_short Stem cell-based gene delivery mediated by cationic niosomes for bone regeneration
title_full Stem cell-based gene delivery mediated by cationic niosomes for bone regeneration
title_fullStr Stem cell-based gene delivery mediated by cationic niosomes for bone regeneration
title_full_unstemmed Stem cell-based gene delivery mediated by cationic niosomes for bone regeneration
title_sort Stem cell-based gene delivery mediated by cationic niosomes for bone regeneration
dc.creator.none.fl_str_mv Attia, Noha
Mashal, Mohamed
Grijalvo, Santiago
Eritja Casadellà, Ramón
Zárate, Jon
Puras, Gustavo
Pedraz, José Luís
author Attia, Noha
author_facet Attia, Noha
Mashal, Mohamed
Grijalvo, Santiago
Eritja Casadellà, Ramón
Zárate, Jon
Puras, Gustavo
Pedraz, José Luís
author_role author
author2 Mashal, Mohamed
Grijalvo, Santiago
Eritja Casadellà, Ramón
Zárate, Jon
Puras, Gustavo
Pedraz, José Luís
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bone regeneration
Gene delivery
Niosomes
Stem cells
topic Bone regeneration
Gene delivery
Niosomes
Stem cells
description Bone morphogenetic protein-7(BMP-7) plays a pivotal role in the transformation of mesenchymal stem cells (MSCs) into bone. However, its impact is hampered due to its short half-life. Therefore, gene therapy may be an interesting approach to deliver BMP-7 gene to D1-MSCs. In this manuscript we prepared and characterized niosomes based on cationic lipid 2,3-di(tetradecyloxy)propan-1-amine, combined with polysorbate 80 for gene delivery purposes. Niosomes were characterized and combined initially with pCMS-EGFP reporter plasmid, and later with pUNO1-hBMP-7 plasmid to evaluate osteogenesis differentiation. Additionally, specific blockers of most relevant endocytic pathways were used to evaluate the intracellular disposition of complexes. MSCs transfected with niosomes showed increased growth rate, enhanced alkaline phosphatase activity (ALP) and extracellular matrix deposition which suggested the formation of osteoblast-like cells. We concluded that hBMP-7-transfected MSCs could be considered not only as an effective delivery tool of hBMP-7, but also as proliferating and bone forming cells for bone regeneration.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/159063
url http://hdl.handle.net/10261/159063
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv 10.1016/j.nano.2017.11.005
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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