Design and Validation of a Process Based on Cationic Niosomes for Gene Delivery into Novel Urine-Derived Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are stem cells present in adult tissues. They can be cultured, have great growth capacity, and can differentiate into several cell types. The isolation of urine-derived mesenchymal stem cells (hUSCs) was recently described. hUSCs present additional benefits in the fact...

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Detalles Bibliográficos
Autores: Vado, Yerai, Puras, Gustavo, Rosique, Melania, Martín, César, Pedraz, José Luis, Jebari-Benslaiman, Shifa, Pancorbo, Marian M. de, Zárate, Jon, Pérez de Nanclares, Guiomar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/310391
Acceso en línea:http://hdl.handle.net/10261/310391
https://api.elsevier.com/content/abstract/scopus_id/85106893050
Access Level:acceso abierto
Palabra clave:Cationic niosomes
Gene therapy
Nonviral vectors
Primary cell culture
Urine-derived mesenchymal stem cells
Descripción
Sumario:Mesenchymal stem cells (MSCs) are stem cells present in adult tissues. They can be cultured, have great growth capacity, and can differentiate into several cell types. The isolation of urine-derived mesenchymal stem cells (hUSCs) was recently described. hUSCs present additional benefits in the fact that they can be easily obtained noninvasively. Regarding gene delivery, nonviral vectors based on cationic niosomes have been used and are more stable and have lower immunogenicity than viral vectors. However, their transfection efficiency is low and in need of improvement.