Binge ethanol and MDMA combination exacerbates toxic cardiac effects by inducing cellular stress

Binge drinking is a common pattern of ethanol consumption among young people. Binge drinkers are especially susceptible to brain damage when other substances are co-administered, in particular 3,4 methylendioxymethamphetamine (MDMA). The aim of the present work was to study the mechanisms implicated...

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Autores: Navarro Zaragoza, Javier, Ros Simó, Clara, Milanés, María Victoria, Valverde Granados, Olga, Laorden, María Luisa
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/25709
Acceso en línea:http://hdl.handle.net/10230/25709
http://dx.doi.org/10.1371/journal.pone.0141502
Access Level:acceso abierto
Palabra clave:MDMA (Droga)
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spelling Binge ethanol and MDMA combination exacerbates toxic cardiac effects by inducing cellular stressNavarro Zaragoza, JavierRos Simó, ClaraMilanés, María VictoriaValverde Granados, OlgaLaorden, María LuisaMDMA (Droga)Binge drinking is a common pattern of ethanol consumption among young people. Binge drinkers are especially susceptible to brain damage when other substances are co-administered, in particular 3,4 methylendioxymethamphetamine (MDMA). The aim of the present work was to study the mechanisms implicated in the adaptive changes observed after administration of these drugs of abuse. So, we have evaluated the cardiac sympathetic activity and the expression and activation of heat shock protein 27 (HSP27), after voluntary binge ethanol consumption, alone and in combination with MDMA. Both parameters are markers of stressful situations and they could be modified inducing several alterations in different systems. Adolescent mice received MDMA, ethanol or both (ethanol plus MDMA). Drinking in the dark (DID) procedure was used as a model of binge. Noradrenaline (NA) turnover, tyrosine hydroxylase (TH), TH phosphorylated at serine 31 and HSP27 expression and its phosphorylation at serine 82 were evaluated in adolescent mice 48 h, 72 h, and 7 days after treatments in the left ventricle. NA and normetanephrine (NMN) were determined by high-performance liquid chromatography (HPLC); TH and HSP27 expression and phosphorylation were measured by quantitative blot immunollabeling using specific antibodies. Ethanol and MDMA co-administration increased NA turnover and TH expression and phosphorylation versus the consumption of each one of these drugs. In parallel with the described modifications in the cardiac sympathetic activity, our results showed that binge ethanol+MDMA exposure is associated with an increase in HSP27 expression and phosphorylation in the left ventricle, supporting the idea that the combination of both drugs exacerbates the cellular stress induced by ethanol or MDMA alone.The funders of this research have been Ministerio de Economía y Competitividad (Grant: SAF2013/49076) and Instituto de Salud Carlos III, Ministerio de Sanidad (Grant: RD12/0028/0003).Public Library of Science (PLoS)201620162015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/25709http://dx.doi.org/10.1371/journal.pone.0141502reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésPLoS One. 2015;10(10):e0141502info:eu-repo/grantAgreement/ES/1PE/SAF2013-49076© 2015 Navarro-Zaragoza et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/257092026-06-12T07:21:37Z
dc.title.none.fl_str_mv Binge ethanol and MDMA combination exacerbates toxic cardiac effects by inducing cellular stress
title Binge ethanol and MDMA combination exacerbates toxic cardiac effects by inducing cellular stress
spellingShingle Binge ethanol and MDMA combination exacerbates toxic cardiac effects by inducing cellular stress
Navarro Zaragoza, Javier
MDMA (Droga)
title_short Binge ethanol and MDMA combination exacerbates toxic cardiac effects by inducing cellular stress
title_full Binge ethanol and MDMA combination exacerbates toxic cardiac effects by inducing cellular stress
title_fullStr Binge ethanol and MDMA combination exacerbates toxic cardiac effects by inducing cellular stress
title_full_unstemmed Binge ethanol and MDMA combination exacerbates toxic cardiac effects by inducing cellular stress
title_sort Binge ethanol and MDMA combination exacerbates toxic cardiac effects by inducing cellular stress
dc.creator.none.fl_str_mv Navarro Zaragoza, Javier
Ros Simó, Clara
Milanés, María Victoria
Valverde Granados, Olga
Laorden, María Luisa
author Navarro Zaragoza, Javier
author_facet Navarro Zaragoza, Javier
Ros Simó, Clara
Milanés, María Victoria
Valverde Granados, Olga
Laorden, María Luisa
author_role author
author2 Ros Simó, Clara
Milanés, María Victoria
Valverde Granados, Olga
Laorden, María Luisa
author2_role author
author
author
author
dc.subject.none.fl_str_mv MDMA (Droga)
topic MDMA (Droga)
description Binge drinking is a common pattern of ethanol consumption among young people. Binge drinkers are especially susceptible to brain damage when other substances are co-administered, in particular 3,4 methylendioxymethamphetamine (MDMA). The aim of the present work was to study the mechanisms implicated in the adaptive changes observed after administration of these drugs of abuse. So, we have evaluated the cardiac sympathetic activity and the expression and activation of heat shock protein 27 (HSP27), after voluntary binge ethanol consumption, alone and in combination with MDMA. Both parameters are markers of stressful situations and they could be modified inducing several alterations in different systems. Adolescent mice received MDMA, ethanol or both (ethanol plus MDMA). Drinking in the dark (DID) procedure was used as a model of binge. Noradrenaline (NA) turnover, tyrosine hydroxylase (TH), TH phosphorylated at serine 31 and HSP27 expression and its phosphorylation at serine 82 were evaluated in adolescent mice 48 h, 72 h, and 7 days after treatments in the left ventricle. NA and normetanephrine (NMN) were determined by high-performance liquid chromatography (HPLC); TH and HSP27 expression and phosphorylation were measured by quantitative blot immunollabeling using specific antibodies. Ethanol and MDMA co-administration increased NA turnover and TH expression and phosphorylation versus the consumption of each one of these drugs. In parallel with the described modifications in the cardiac sympathetic activity, our results showed that binge ethanol+MDMA exposure is associated with an increase in HSP27 expression and phosphorylation in the left ventricle, supporting the idea that the combination of both drugs exacerbates the cellular stress induced by ethanol or MDMA alone.
publishDate 2015
dc.date.none.fl_str_mv 2015
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/25709
http://dx.doi.org/10.1371/journal.pone.0141502
url http://hdl.handle.net/10230/25709
http://dx.doi.org/10.1371/journal.pone.0141502
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv PLoS One. 2015;10(10):e0141502
info:eu-repo/grantAgreement/ES/1PE/SAF2013-49076
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
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