Early markers are present in both embryogenesis pathways from microspores and immature zygotic embryos in cork oak, Quercus suber L

Background: In Quercus suber, cork oak, a Mediterranean forest tree of economic and social interest, rapid production of isogenic lines and clonal propagation of elite genotypes have been achieved by developing in vitro embryogenesis from microspores and zygotic embryos respectively. Despite its hig...

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Autores: Rodríguez-Sanz, Héctor, Manzanera, José Antonio, Solís González, María Teresa, Gómez-Garay, Aranzazu, Pintos López, Beatriz, Risueño, María C., Testillano, Pilar S.
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/35586
Acceso en línea:https://hdl.handle.net/20.500.14352/35586
Access Level:acceso abierto
Palabra clave:581.526.42
581.3
Somatic embryogenesis
Microspore embryogenesis
Anther culture
DNA methylation
5-methyl-deoxy-cytidine
Pectin esterification
Cell wall
Auxin
IAA
Botánica (Biología)
2417.03 Botánica General
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oai_identifier_str oai:docta.ucm.es:20.500.14352/35586
network_acronym_str ES
network_name_str España
repository_id_str
spelling Early markers are present in both embryogenesis pathways from microspores and immature zygotic embryos in cork oak, Quercus suber LRodríguez-Sanz, HéctorManzanera, José AntonioSolís González, María TeresaGómez-Garay, AranzazuPintos López, BeatrizRisueño, María C.Testillano, Pilar S.581.526.42581.3Somatic embryogenesisMicrospore embryogenesisAnther cultureDNA methylation5-methyl-deoxy-cytidinePectin esterificationCell wallAuxinIAABotánica (Biología)2417.03 Botánica GeneralBackground: In Quercus suber, cork oak, a Mediterranean forest tree of economic and social interest, rapid production of isogenic lines and clonal propagation of elite genotypes have been achieved by developing in vitro embryogenesis from microspores and zygotic embryos respectively. Despite its high potential in tree breeding strategies, due to their recalcitrancy, the efficiency of embryogenesis in vitro systems in many woody species is still very low since factors responsible for embryogenesis initiation and embryo development are still largely unknown. The search for molecular and cellular markers during early stages of in vitro embryogenesis constitutes an important goal to distinguish, after induction, responsive from non-responsive cells, and to elucidate the mechanisms involved in embryogenesis initiation for their efficient manipulation. In this work, we have performed a comparative analysis of two embryogenesis pathways derived from microspores and immature zygotic embryos in cork oak in order to characterize early markers of reprogrammed cells in both pathways. Rearrangements of the cell structural organization, changes in epigenetic marks, cell wall polymers modifications and endogenous auxin changes were analyzed at early embryogenesis stages of the two in vitro systems by a multidisciplinary approach. Results: Results showed that early embryo cells exhibited defined changes of cell components which were similar in both embryogenesis in vitro systems, cellular features that were not found in non-embryogenic cells. DNA methylation level and nuclear pattern, proportion of esterified pectins in cell walls, and endogenous auxin levels were different in embryo cells in comparison with microspores and immature zygotic embryo cells from which embryos originated, constituting early embryogenesis markers. Conclusions: These findings suggest that DNA hypomethylation, cell wall remodeling by pectin esterification and auxin increase are involved in early in vitro embryogenesis in woody species, providing new evidences of the developmental pattern similarity between both embryogenesis pathways, from microspores and immature zygotic embryos, in woody species.BIoMed CentralUniversidad Complutense de Madrid20142014-01-0120142014-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/35586reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/355862026-06-02T12:44:21Z
dc.title.none.fl_str_mv Early markers are present in both embryogenesis pathways from microspores and immature zygotic embryos in cork oak, Quercus suber L
title Early markers are present in both embryogenesis pathways from microspores and immature zygotic embryos in cork oak, Quercus suber L
spellingShingle Early markers are present in both embryogenesis pathways from microspores and immature zygotic embryos in cork oak, Quercus suber L
Rodríguez-Sanz, Héctor
581.526.42
581.3
Somatic embryogenesis
Microspore embryogenesis
Anther culture
DNA methylation
5-methyl-deoxy-cytidine
Pectin esterification
Cell wall
Auxin
IAA
Botánica (Biología)
2417.03 Botánica General
title_short Early markers are present in both embryogenesis pathways from microspores and immature zygotic embryos in cork oak, Quercus suber L
title_full Early markers are present in both embryogenesis pathways from microspores and immature zygotic embryos in cork oak, Quercus suber L
title_fullStr Early markers are present in both embryogenesis pathways from microspores and immature zygotic embryos in cork oak, Quercus suber L
title_full_unstemmed Early markers are present in both embryogenesis pathways from microspores and immature zygotic embryos in cork oak, Quercus suber L
title_sort Early markers are present in both embryogenesis pathways from microspores and immature zygotic embryos in cork oak, Quercus suber L
dc.creator.none.fl_str_mv Rodríguez-Sanz, Héctor
Manzanera, José Antonio
Solís González, María Teresa
Gómez-Garay, Aranzazu
Pintos López, Beatriz
Risueño, María C.
Testillano, Pilar S.
author Rodríguez-Sanz, Héctor
author_facet Rodríguez-Sanz, Héctor
Manzanera, José Antonio
Solís González, María Teresa
Gómez-Garay, Aranzazu
Pintos López, Beatriz
Risueño, María C.
Testillano, Pilar S.
author_role author
author2 Manzanera, José Antonio
Solís González, María Teresa
Gómez-Garay, Aranzazu
Pintos López, Beatriz
Risueño, María C.
Testillano, Pilar S.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 581.526.42
581.3
Somatic embryogenesis
Microspore embryogenesis
Anther culture
DNA methylation
5-methyl-deoxy-cytidine
Pectin esterification
Cell wall
Auxin
IAA
Botánica (Biología)
2417.03 Botánica General
topic 581.526.42
581.3
Somatic embryogenesis
Microspore embryogenesis
Anther culture
DNA methylation
5-methyl-deoxy-cytidine
Pectin esterification
Cell wall
Auxin
IAA
Botánica (Biología)
2417.03 Botánica General
description Background: In Quercus suber, cork oak, a Mediterranean forest tree of economic and social interest, rapid production of isogenic lines and clonal propagation of elite genotypes have been achieved by developing in vitro embryogenesis from microspores and zygotic embryos respectively. Despite its high potential in tree breeding strategies, due to their recalcitrancy, the efficiency of embryogenesis in vitro systems in many woody species is still very low since factors responsible for embryogenesis initiation and embryo development are still largely unknown. The search for molecular and cellular markers during early stages of in vitro embryogenesis constitutes an important goal to distinguish, after induction, responsive from non-responsive cells, and to elucidate the mechanisms involved in embryogenesis initiation for their efficient manipulation. In this work, we have performed a comparative analysis of two embryogenesis pathways derived from microspores and immature zygotic embryos in cork oak in order to characterize early markers of reprogrammed cells in both pathways. Rearrangements of the cell structural organization, changes in epigenetic marks, cell wall polymers modifications and endogenous auxin changes were analyzed at early embryogenesis stages of the two in vitro systems by a multidisciplinary approach. Results: Results showed that early embryo cells exhibited defined changes of cell components which were similar in both embryogenesis in vitro systems, cellular features that were not found in non-embryogenic cells. DNA methylation level and nuclear pattern, proportion of esterified pectins in cell walls, and endogenous auxin levels were different in embryo cells in comparison with microspores and immature zygotic embryo cells from which embryos originated, constituting early embryogenesis markers. Conclusions: These findings suggest that DNA hypomethylation, cell wall remodeling by pectin esterification and auxin increase are involved in early in vitro embryogenesis in woody species, providing new evidences of the developmental pattern similarity between both embryogenesis pathways, from microspores and immature zygotic embryos, in woody species.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01
2014
2014-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/35586
url https://hdl.handle.net/20.500.14352/35586
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BIoMed Central
publisher.none.fl_str_mv BIoMed Central
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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