A new microspore embryogenesis system under low temperature which mimics zygotic embryogenesis initials, expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napus
Microspore embryogenesis represents a unique system of single cell reprogramming in plants wherein a highly specialized cell, the microspore, by specific stress treatment, switches its fate towards an embryogenesis pathway. In Brassica napus, a model species for this phenomenon, incubation of isolat...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2012 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/95748 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/95748 |
| Access Level: | acceso abierto |
| Palabra clave: | 576.32 Microspore embryogenesis Zygotic-like microspore embryogenesis Suspensor-like Brassica napus Doubled-haploid Embryo germination Plant regeneration In vitro microspore culture Auxin IAA Biología celular (Biología) Biotecnología 31 Ciencias Agrarias 2407 Biología Celular |
| Sumario: | Microspore embryogenesis represents a unique system of single cell reprogramming in plants wherein a highly specialized cell, the microspore, by specific stress treatment, switches its fate towards an embryogenesis pathway. In Brassica napus, a model species for this phenomenon, incubation of isolated microspores at 32°C is considered to be a pre-requisite for embryogenesis induction. |
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