Remote Cell Growth Sensing Using Self-Sustained Bio-Oscillations

A smart sensor system for cell culture real-time supervision is proposed, allowing for a significant reduction in human effort applied to this type of assay. The approach converts the cell culture under test into a suitable “biological” oscillator. The system enables the remote acquisition and manag...

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Detalles Bibliográficos
Autores: Pérez, Pablo, Huertas, Gloria, Olmo, Alberto, Maldonado-Jacobi, Andrés, Serrano, Juan Alfonso, Martín-Rubio, María E., Daza, Paula, Yúfera, Alberto
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/169026
Acceso en línea:http://hdl.handle.net/10261/169026
Access Level:acceso abierto
Palabra clave:Smart sensing
Bioimpedance
Cell culture
OBT
Real-time monitoring
Descripción
Sumario:A smart sensor system for cell culture real-time supervision is proposed, allowing for a significant reduction in human effort applied to this type of assay. The approach converts the cell culture under test into a suitable “biological” oscillator. The system enables the remote acquisition and management of the “biological” oscillation signals through a secure web interface. The indirectly observed biological properties are cell growth and cell number, which are straightforwardly related to the measured bio-oscillation signal parameters, i.e., frequency and amplitude. The sensor extracts the information without complex circuitry for acquisition and measurement, taking advantage of the microcontroller features. A discrete prototype for sensing and remote monitoring is presented along with the experimental results obtained from the performed measurements, achieving the expected performance and outcomes.