Alternative General Fitting Methods for Real-Time Cell-Count Experimental Data Processing
This paper reports two general methods for extraction of cell-electrode electrical model parameters in cell culture (CC) assays. The presented approaches can be applied to CC assays based on electrical cell-substrate impedance spectroscopy (ECIS) technique for real-time supervision, providing the ce...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión enviada para evaluación y publicación |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/106614 |
| Acceso en línea: | https://hdl.handle.net/11441/106614 https://doi.org/10.1109/JSEN.2020.3010292 |
| Access Level: | acceso abierto |
| Palabra clave: | Bioimpedance Cell culture ECIS Electrical electrode model OBT |
| Sumario: | This paper reports two general methods for extraction of cell-electrode electrical model parameters in cell culture (CC) assays. The presented approaches can be applied to CC assays based on electrical cell-substrate impedance spectroscopy (ECIS) technique for real-time supervision, providing the cell number per square centimeter, i.e., the cell density, asmain result.Both of the proposedmethods - minimization of system equations and data predictive model - search, during the experiment, the optimum values of the electrical model parameters employed for the electrodecellmodel. The results of this searchenablea fast and efficient calculation of the involved cell-electrode model parameters and supply real-time information on the cell number. For the sake of experimental validation, we applied the proposed methods to practical CCs in cell growth assays with a cell line of AA8 Chinese hamster ovarian fibroblasts and the Oscillation Based Test technique for bioimpedance measurements. These methods can be easily extrapolated to any general cell lines and/or other bioimpedance test methodologies. |
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