On interrogating electron microscopy images to discover proteins in the cell

Interrogating individual two-dimensional (2D) cryo-EM images for the presence of defined three-dimensional (3D) structures that correspond to previously known (or predicted) macromolecular complexes is very challenging, but offers attractive opportunities for the analysis of large numbers of specime...

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Detalhes bibliográficos
Autor: Carazo, Jose M.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/391071
Acesso em linha:http://hdl.handle.net/10261/391071
https://api.elsevier.com/content/abstract/scopus_id/86000669764
Access Level:acceso abierto
Palavra-chave:Cryo-EM
Cryo-electron microscopy
Image processing
Protein structure
Template matching
Descrição
Resumo:Interrogating individual two-dimensional (2D) cryo-EM images for the presence of defined three-dimensional (3D) structures that correspond to previously known (or predicted) macromolecular complexes is very challenging, but offers attractive opportunities for the analysis of large numbers of specimens. The work of Zhang et al. [(2025), IUCrJ, 12, 155-176] represents a significant step forward towards this goal.