Recruitment of reverse transcriptase-Cas1 fusion proteins by Type VI-A CRISPR-Cas systems

Type VI CRISPR–Cas systems contain a single effector nuclease (Cas13) that exclusively targets single-stranded RNA. It remains unknown how these systems acquire spacers. It has been suggested that type VI systems with adaptation modules can acquire spacers from RNA bacteriophages, but sequence simil...

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Detalles Bibliográficos
Autores: Toro, Nicolás, Mestre, M.R., Martínez-Abarca, Francisco, González-Delgado, Alejandro
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/202024
Acceso en línea:http://hdl.handle.net/10261/202024
Access Level:acceso abierto
Palabra clave:Reverse transcriptase
CRISPR-Cas
Phylogeny
Type VI CRISPR
Cas1
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spelling Recruitment of reverse transcriptase-Cas1 fusion proteins by Type VI-A CRISPR-Cas systemsToro, NicolásMestre, M.R.Martínez-Abarca, FranciscoGonzález-Delgado, AlejandroReverse transcriptaseCRISPR-CasPhylogenyType VI CRISPRCas1Type VI CRISPR–Cas systems contain a single effector nuclease (Cas13) that exclusively targets single-stranded RNA. It remains unknown how these systems acquire spacers. It has been suggested that type VI systems with adaptation modules can acquire spacers from RNA bacteriophages, but sequence similarities suggest that spacers may provide immunity to DNA phages. We searched databases for Cas13 proteins with linked RTs. We identified two different type VI-A systems with adaptation modules including an RT-Cas1 fusion and Cas2 proteins. Phylogenetic reconstruction analyses revealed that these adaptation modules were recruited by different effector Cas13a proteins, possibly from RT-associated type III-D systems within the bacterial classes Alphaproteobacteria and Clostridia. These type VI-A systems are predicted to acquire spacers from RNA molecules, paving the way for future studies investigating their role in bacterial adaptive immunity and biotechnological applications.This work was supported by the Spanish Ministerio de Ciencia, Innovación y Universidades, including ERDF (European Regional Development Funds) research grants (BIO2014-51953-P and BIO2017-82244-P). AG-D was supported by a FPU predoctoral fellowship grant from the Ministerio de Economía y Competitividad (FPU15/02714).Frontiers MediaMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)European CommissionMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020192020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/202024reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2014-51953-PBIO2017-82244-P/AEI/10.13039/501100011033info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BIO2017-82244-Phttp://dx.doi.org/10.3389/fmicb.2019.02160Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2020242026-05-22T06:33:51Z
dc.title.none.fl_str_mv Recruitment of reverse transcriptase-Cas1 fusion proteins by Type VI-A CRISPR-Cas systems
title Recruitment of reverse transcriptase-Cas1 fusion proteins by Type VI-A CRISPR-Cas systems
spellingShingle Recruitment of reverse transcriptase-Cas1 fusion proteins by Type VI-A CRISPR-Cas systems
Toro, Nicolás
Reverse transcriptase
CRISPR-Cas
Phylogeny
Type VI CRISPR
Cas1
title_short Recruitment of reverse transcriptase-Cas1 fusion proteins by Type VI-A CRISPR-Cas systems
title_full Recruitment of reverse transcriptase-Cas1 fusion proteins by Type VI-A CRISPR-Cas systems
title_fullStr Recruitment of reverse transcriptase-Cas1 fusion proteins by Type VI-A CRISPR-Cas systems
title_full_unstemmed Recruitment of reverse transcriptase-Cas1 fusion proteins by Type VI-A CRISPR-Cas systems
title_sort Recruitment of reverse transcriptase-Cas1 fusion proteins by Type VI-A CRISPR-Cas systems
dc.creator.none.fl_str_mv Toro, Nicolás
Mestre, M.R.
Martínez-Abarca, Francisco
González-Delgado, Alejandro
author Toro, Nicolás
author_facet Toro, Nicolás
Mestre, M.R.
Martínez-Abarca, Francisco
González-Delgado, Alejandro
author_role author
author2 Mestre, M.R.
Martínez-Abarca, Francisco
González-Delgado, Alejandro
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
European Commission
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Reverse transcriptase
CRISPR-Cas
Phylogeny
Type VI CRISPR
Cas1
topic Reverse transcriptase
CRISPR-Cas
Phylogeny
Type VI CRISPR
Cas1
description Type VI CRISPR–Cas systems contain a single effector nuclease (Cas13) that exclusively targets single-stranded RNA. It remains unknown how these systems acquire spacers. It has been suggested that type VI systems with adaptation modules can acquire spacers from RNA bacteriophages, but sequence similarities suggest that spacers may provide immunity to DNA phages. We searched databases for Cas13 proteins with linked RTs. We identified two different type VI-A systems with adaptation modules including an RT-Cas1 fusion and Cas2 proteins. Phylogenetic reconstruction analyses revealed that these adaptation modules were recruited by different effector Cas13a proteins, possibly from RT-associated type III-D systems within the bacterial classes Alphaproteobacteria and Clostridia. These type VI-A systems are predicted to acquire spacers from RNA molecules, paving the way for future studies investigating their role in bacterial adaptive immunity and biotechnological applications.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/202024
url http://hdl.handle.net/10261/202024
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2014-51953-P
BIO2017-82244-P/AEI/10.13039/501100011033
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BIO2017-82244-P
http://dx.doi.org/10.3389/fmicb.2019.02160
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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