A Three-Protein Panel to Support the Diagnosis of Sepsis in Children

Sepsis is a syndrome without a standard validated diagnostic test. Early recognition is crucial. Serum proteome analysis in children with sepsis may identify new biomarkers. This study aimed to find suitable blood biomarkers for an early diagnosis of sepsis. An analytical observational case-control...

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Autores: Pilar Orive, Francisco Javier, Astigarraga Aguirre, María Iciar, Azkargorta, Mikel, Elortza, Felix, García Obregón, Susana
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/57839
Acceso en línea:http://hdl.handle.net/10810/57839
Access Level:acceso abierto
Palabra clave:mass spectrometry analysis
proteome
sepsis
septic shock
children
biomarkers
campaign international guidelines
management
proteomics
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spelling A Three-Protein Panel to Support the Diagnosis of Sepsis in ChildrenPilar Orive, Francisco JavierAstigarraga Aguirre, María IciarAzkargorta, MikelElortza, FelixGarcía Obregón, Susanamass spectrometry analysisproteomesepsisseptic shockchildrenbiomarkerscampaign international guidelinesmanagementproteomicsSepsis is a syndrome without a standard validated diagnostic test. Early recognition is crucial. Serum proteome analysis in children with sepsis may identify new biomarkers. This study aimed to find suitable blood biomarkers for an early diagnosis of sepsis. An analytical observational case-control study was carried out in a single center. Children admitted to a Pediatric Intensive Care Unit with clinical diagnosed sepsis were eligible for study. A proteomic analysis conducted by mass spectrometry was performed. Forty patients with sepsis and 24 healthy donors were recruited. Proteomics results revealed 44 proteins differentially expressed between patients and healthy controls. Six proteins were selected to be validated: lactoferrin, serum amyloid-A1 (SAA-1), complement factor B, leucine-rich alpha-2 glycoprotein (LRG1), soluble interleukin-2 alpha chain receptor (sCD25) and soluble haptoglobin-hemoglobin receptor. Our results showed that sCD25, SAA-1, and LRG1 had high levels of specificity and sensitivity, as well as an excellent area under the ROC curve (>0.9). Our study provides a serum proteomic analysis that identifies new diagnostic biomarkers in sepsis. SAA-1, sCD25 and LRG1 were able to separate septic from healthy donor, so they could be used together with other clinical and analytical features to improve sepsis diagnosis in children.This work was funded by Research Projects from University of Basque Country (US10/02) and from the Basque Government (SAIO10-PE10BF02, SAIO12-PE12BF002, 2012111052, 2019111056). CICbioGUNE is supported by Basque Department of Industry, Tourism and Trade (Etortek and Elkartek programs), the Innovation Technology Department of the Bizkaia County, the ProteoRed-ISCIII (Grant PRB3 IPT17/0019), CIBERehd Network and Severo Ochoa Grant (SEV-2016-0644).MDPI202220222022info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/57839reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://www.mdpi.com/2077-0383/11/6/1563/htminfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).Atribución 3.0 Españaoai:addi.ehu.eus:10810/578392026-06-18T09:23:17Z
dc.title.none.fl_str_mv A Three-Protein Panel to Support the Diagnosis of Sepsis in Children
title A Three-Protein Panel to Support the Diagnosis of Sepsis in Children
spellingShingle A Three-Protein Panel to Support the Diagnosis of Sepsis in Children
Pilar Orive, Francisco Javier
mass spectrometry analysis
proteome
sepsis
septic shock
children
biomarkers
campaign international guidelines
management
proteomics
title_short A Three-Protein Panel to Support the Diagnosis of Sepsis in Children
title_full A Three-Protein Panel to Support the Diagnosis of Sepsis in Children
title_fullStr A Three-Protein Panel to Support the Diagnosis of Sepsis in Children
title_full_unstemmed A Three-Protein Panel to Support the Diagnosis of Sepsis in Children
title_sort A Three-Protein Panel to Support the Diagnosis of Sepsis in Children
dc.creator.none.fl_str_mv Pilar Orive, Francisco Javier
Astigarraga Aguirre, María Iciar
Azkargorta, Mikel
Elortza, Felix
García Obregón, Susana
author Pilar Orive, Francisco Javier
author_facet Pilar Orive, Francisco Javier
Astigarraga Aguirre, María Iciar
Azkargorta, Mikel
Elortza, Felix
García Obregón, Susana
author_role author
author2 Astigarraga Aguirre, María Iciar
Azkargorta, Mikel
Elortza, Felix
García Obregón, Susana
author2_role author
author
author
author
dc.subject.none.fl_str_mv mass spectrometry analysis
proteome
sepsis
septic shock
children
biomarkers
campaign international guidelines
management
proteomics
topic mass spectrometry analysis
proteome
sepsis
septic shock
children
biomarkers
campaign international guidelines
management
proteomics
description Sepsis is a syndrome without a standard validated diagnostic test. Early recognition is crucial. Serum proteome analysis in children with sepsis may identify new biomarkers. This study aimed to find suitable blood biomarkers for an early diagnosis of sepsis. An analytical observational case-control study was carried out in a single center. Children admitted to a Pediatric Intensive Care Unit with clinical diagnosed sepsis were eligible for study. A proteomic analysis conducted by mass spectrometry was performed. Forty patients with sepsis and 24 healthy donors were recruited. Proteomics results revealed 44 proteins differentially expressed between patients and healthy controls. Six proteins were selected to be validated: lactoferrin, serum amyloid-A1 (SAA-1), complement factor B, leucine-rich alpha-2 glycoprotein (LRG1), soluble interleukin-2 alpha chain receptor (sCD25) and soluble haptoglobin-hemoglobin receptor. Our results showed that sCD25, SAA-1, and LRG1 had high levels of specificity and sensitivity, as well as an excellent area under the ROC curve (>0.9). Our study provides a serum proteomic analysis that identifies new diagnostic biomarkers in sepsis. SAA-1, sCD25 and LRG1 were able to separate septic from healthy donor, so they could be used together with other clinical and analytical features to improve sepsis diagnosis in children.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/57839
url http://hdl.handle.net/10810/57839
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.mdpi.com/2077-0383/11/6/1563/htm
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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