Long-range guidance of spinal commissural axons by netrin1 and sonic hedgehog from midline floor plate cells

An important model for axon pathfinding is provided by guidance of embryonic commissural axons from dorsal spinal cord to ventral midline floor plate (FP). FP cells produce a chemoattractive activity, comprised largely of netrin1 (FP-netrin1) and Sonic hedgehog (Shh), that can attract the axons at a...

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Autores: Wu, Zhuhao, Makihara, Shirin, Yam, Patricia T., Teo, Shaun, Renier, Nicolas, Balekoglu, Nursen, Moreno-Bravo, Juan Antonio, Olsen, Olav, Chédotal, Alain, Charron, Frédéric, Tessier-Lavigne, Marc
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/308987
Acesso em linha:http://hdl.handle.net/10261/308987
Access Level:acceso abierto
Palavra-chave:Axon guidance
Long-range guidance
Netrin1
Sonic hedgehog
Chemoattraction
Haptotaxis
Commissural neurons
Spinal cord
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spelling Long-range guidance of spinal commissural axons by netrin1 and sonic hedgehog from midline floor plate cellsWu, ZhuhaoMakihara, ShirinYam, Patricia T.Teo, ShaunRenier, NicolasBalekoglu, NursenMoreno-Bravo, Juan AntonioOlsen, OlavChédotal, AlainCharron, FrédéricTessier-Lavigne, MarcAxon guidanceLong-range guidanceNetrin1Sonic hedgehogChemoattractionHaptotaxisCommissural neuronsSpinal cordAn important model for axon pathfinding is provided by guidance of embryonic commissural axons from dorsal spinal cord to ventral midline floor plate (FP). FP cells produce a chemoattractive activity, comprised largely of netrin1 (FP-netrin1) and Sonic hedgehog (Shh), that can attract the axons at a distance in vitro. netrin1 is also produced by ventricular zone (VZ) progenitors along the axons’ route (VZ-netrin1). Recent studies using region-specific netrin1 deletion suggested that FP-netrin1 is dispensable and VZ-netrin1 sufficient for netrin guidance activity in vivo. We show that removing FP-netrin1 actually causes guidance defects in spinal cord consistent with long-range action (i.e., over hundreds of micrometers), and double mutant analysis supports that FP-netrin1 and Shh collaborate to attract at long range. We further provide evidence that netrin1 may guide via chemotaxis or haptotaxis. These results support the model that netrin1 signals at both short and long range to guide commissural axons in spinal cord.Z.W. was supported by the Kavli Neural Systems Institute at The Rockefeller University. S.M. was supported by a Keidanren Ishizaka Memorial Foundation fellowship. S.T. was supported by fellowship funds from the Agency for Science, Technology and Research, Singapore (A∗STAR). N.R. was supported by a post-doctoral fellowship from the Shelby White – Leon Levy Foundation. Work performed in the M.T.-L. laboratory was supported by The Rockefeller University and Stanford University, work performed in the A.C. laboratory was supported by a grant from the Agence Nationale de la Recherche (ANR-14-CE13-0004-01), and work performed in the F.C. laboratory was supported by the Canadian Institutes of Health Research (CIHR FDN334023), the Fonds de Recherche du Québec - Santé (FRQS), and the Canada Foundation for Innovation (CFI 33768). F.C. holds the Canada Research Chair in Developmental Neurobiology.Peer reviewedElsevierKavli FoundationRockefeller UniversityAgency for Science, Technology and Research A*STAR (Singapore)Leon Levy FoundationStanford UniversityAgence Nationale de la Recherche (France)Canadian Institutes of Health ResearchCanada Foundation for Innovation202320232019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/308987reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésNeuronSynergistic Activity of Floor-Plate- and Ventricular-Zone-Derived Netrin-1 in Spinal Cord Commissural Axon Guidance Neuron, Volume 101, Issue 4, 20 February 2019, Pages 625-634.e3 Juan Antonio Moreno-Bravo, Sergi Roig Puiggros, Patrick Mehlen, Alain Chédotalhttps://doi.org/10.1016/j.neuron.2018.12.025Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3089872026-05-22T06:33:51Z
dc.title.none.fl_str_mv Long-range guidance of spinal commissural axons by netrin1 and sonic hedgehog from midline floor plate cells
title Long-range guidance of spinal commissural axons by netrin1 and sonic hedgehog from midline floor plate cells
spellingShingle Long-range guidance of spinal commissural axons by netrin1 and sonic hedgehog from midline floor plate cells
Wu, Zhuhao
Axon guidance
Long-range guidance
Netrin1
Sonic hedgehog
Chemoattraction
Haptotaxis
Commissural neurons
Spinal cord
title_short Long-range guidance of spinal commissural axons by netrin1 and sonic hedgehog from midline floor plate cells
title_full Long-range guidance of spinal commissural axons by netrin1 and sonic hedgehog from midline floor plate cells
title_fullStr Long-range guidance of spinal commissural axons by netrin1 and sonic hedgehog from midline floor plate cells
title_full_unstemmed Long-range guidance of spinal commissural axons by netrin1 and sonic hedgehog from midline floor plate cells
title_sort Long-range guidance of spinal commissural axons by netrin1 and sonic hedgehog from midline floor plate cells
dc.creator.none.fl_str_mv Wu, Zhuhao
Makihara, Shirin
Yam, Patricia T.
Teo, Shaun
Renier, Nicolas
Balekoglu, Nursen
Moreno-Bravo, Juan Antonio
Olsen, Olav
Chédotal, Alain
Charron, Frédéric
Tessier-Lavigne, Marc
author Wu, Zhuhao
author_facet Wu, Zhuhao
Makihara, Shirin
Yam, Patricia T.
Teo, Shaun
Renier, Nicolas
Balekoglu, Nursen
Moreno-Bravo, Juan Antonio
Olsen, Olav
Chédotal, Alain
Charron, Frédéric
Tessier-Lavigne, Marc
author_role author
author2 Makihara, Shirin
Yam, Patricia T.
Teo, Shaun
Renier, Nicolas
Balekoglu, Nursen
Moreno-Bravo, Juan Antonio
Olsen, Olav
Chédotal, Alain
Charron, Frédéric
Tessier-Lavigne, Marc
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Kavli Foundation
Rockefeller University
Agency for Science, Technology and Research A*STAR (Singapore)
Leon Levy Foundation
Stanford University
Agence Nationale de la Recherche (France)
Canadian Institutes of Health Research
Canada Foundation for Innovation
dc.subject.none.fl_str_mv Axon guidance
Long-range guidance
Netrin1
Sonic hedgehog
Chemoattraction
Haptotaxis
Commissural neurons
Spinal cord
topic Axon guidance
Long-range guidance
Netrin1
Sonic hedgehog
Chemoattraction
Haptotaxis
Commissural neurons
Spinal cord
description An important model for axon pathfinding is provided by guidance of embryonic commissural axons from dorsal spinal cord to ventral midline floor plate (FP). FP cells produce a chemoattractive activity, comprised largely of netrin1 (FP-netrin1) and Sonic hedgehog (Shh), that can attract the axons at a distance in vitro. netrin1 is also produced by ventricular zone (VZ) progenitors along the axons’ route (VZ-netrin1). Recent studies using region-specific netrin1 deletion suggested that FP-netrin1 is dispensable and VZ-netrin1 sufficient for netrin guidance activity in vivo. We show that removing FP-netrin1 actually causes guidance defects in spinal cord consistent with long-range action (i.e., over hundreds of micrometers), and double mutant analysis supports that FP-netrin1 and Shh collaborate to attract at long range. We further provide evidence that netrin1 may guide via chemotaxis or haptotaxis. These results support the model that netrin1 signals at both short and long range to guide commissural axons in spinal cord.
publishDate 2019
dc.date.none.fl_str_mv 2019
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/308987
url http://hdl.handle.net/10261/308987
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Neuron
Synergistic Activity of Floor-Plate- and Ventricular-Zone-Derived Netrin-1 in Spinal Cord Commissural Axon Guidance Neuron, Volume 101, Issue 4, 20 February 2019, Pages 625-634.e3 Juan Antonio Moreno-Bravo, Sergi Roig Puiggros, Patrick Mehlen, Alain Chédotal
https://doi.org/10.1016/j.neuron.2018.12.025
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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