HDL anti-inflammatory function is impaired and associated with high SAA1 and low APOA4 levels in aneurysmal subarachnoid hemorrhage

Aneurysmal subarachnoid hemorrhage (aSAH) is a devastating disease with high morbidity and mortality rates. Within 24 hours after aSAH, monocytes are recruited and enter the subarachnoid space, where they mature into macrophages, increasing the inflammatory response and contributing, along with othe...

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Autores: Azúa-López, Zaida Ruiz de, Pezzotti, M Rosa, González-Díaz, Ángela, Meilhac, Olivier, Ureña, Juan, Amaya-Villar, Rosario, Castellano Orozco, Antonio Gonzalo, Varela Pérez, Lourdes María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/167456
Acceso en línea:https://hdl.handle.net/11441/167456
https://doi.org/10.1177/0271678x231184806
Access Level:acceso abierto
Palabra clave:Aneurysmal subarachnoid hemorrhage (aSAH)
Apolipoprotein A4 (APOA4)
High-density lipoproteins (HDL)
Serum amyloid A1 (SAA1)
Vascular cell adhesion protein-1 (VCAM-1)
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spelling HDL anti-inflammatory function is impaired and associated with high SAA1 and low APOA4 levels in aneurysmal subarachnoid hemorrhageAzúa-López, Zaida Ruiz dePezzotti, M RosaGonzález-Díaz, ÁngelaMeilhac, OlivierUreña, JuanAmaya-Villar, RosarioCastellano Orozco, Antonio GonzaloVarela Pérez, Lourdes MaríaAneurysmal subarachnoid hemorrhage (aSAH)Apolipoprotein A4 (APOA4)High-density lipoproteins (HDL)Serum amyloid A1 (SAA1)Vascular cell adhesion protein-1 (VCAM-1)Aneurysmal subarachnoid hemorrhage (aSAH) is a devastating disease with high morbidity and mortality rates. Within 24 hours after aSAH, monocytes are recruited and enter the subarachnoid space, where they mature into macrophages, increasing the inflammatory response and contributing, along with other factors, to delayed neurological dysfunction and poor outcomes. High-density lipoproteins (HDL) are lipid-protein complexes that exert anti-inflammatory effects but under pathological conditions undergo structural alterations that have been associated with loss of functionality. Plasma HDL were isolated from patients with aSAH and analyzed for their anti-inflammatory activity and protein composition. HDLisolated from patients lost the ability to prevent VCAM-1 expression in endothelial cells (HUVEC) and subsequent adhesion of THP-1 monocytes to the endothelium. Proteomic analysis showed that HDL particles from patients had an altered composition compared to those of healthy subjects. We confirmed by western blot that low levels of apolipo protein A4 (APOA4) and high of serum amyloid A1 (SAA1) in HDL were associated with the lack of anti-inflammatory function observed in aSAH. Our results indicate that the study of HDL in the pathophysiology of aSAH is needed, and functional HDL supplementation could be considered a novel therapeutic approach to the treatment of the inflamma tory response after aSAH.SageFisiología Médica y BiofísicaCTS591: Fisiopatología molecular del sistema cardiovascular2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/167456https://doi.org/10.1177/0271678x231184806reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésJournal of cerebral blood flow and metabolism, 43 (11), 1919-1930.https://journals.sagepub.com/doi/10.1177/0271678X231184806info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1674562026-06-17T12:51:07Z
dc.title.none.fl_str_mv HDL anti-inflammatory function is impaired and associated with high SAA1 and low APOA4 levels in aneurysmal subarachnoid hemorrhage
title HDL anti-inflammatory function is impaired and associated with high SAA1 and low APOA4 levels in aneurysmal subarachnoid hemorrhage
spellingShingle HDL anti-inflammatory function is impaired and associated with high SAA1 and low APOA4 levels in aneurysmal subarachnoid hemorrhage
Azúa-López, Zaida Ruiz de
Aneurysmal subarachnoid hemorrhage (aSAH)
Apolipoprotein A4 (APOA4)
High-density lipoproteins (HDL)
Serum amyloid A1 (SAA1)
Vascular cell adhesion protein-1 (VCAM-1)
title_short HDL anti-inflammatory function is impaired and associated with high SAA1 and low APOA4 levels in aneurysmal subarachnoid hemorrhage
title_full HDL anti-inflammatory function is impaired and associated with high SAA1 and low APOA4 levels in aneurysmal subarachnoid hemorrhage
title_fullStr HDL anti-inflammatory function is impaired and associated with high SAA1 and low APOA4 levels in aneurysmal subarachnoid hemorrhage
title_full_unstemmed HDL anti-inflammatory function is impaired and associated with high SAA1 and low APOA4 levels in aneurysmal subarachnoid hemorrhage
title_sort HDL anti-inflammatory function is impaired and associated with high SAA1 and low APOA4 levels in aneurysmal subarachnoid hemorrhage
dc.creator.none.fl_str_mv Azúa-López, Zaida Ruiz de
Pezzotti, M Rosa
González-Díaz, Ángela
Meilhac, Olivier
Ureña, Juan
Amaya-Villar, Rosario
Castellano Orozco, Antonio Gonzalo
Varela Pérez, Lourdes María
author Azúa-López, Zaida Ruiz de
author_facet Azúa-López, Zaida Ruiz de
Pezzotti, M Rosa
González-Díaz, Ángela
Meilhac, Olivier
Ureña, Juan
Amaya-Villar, Rosario
Castellano Orozco, Antonio Gonzalo
Varela Pérez, Lourdes María
author_role author
author2 Pezzotti, M Rosa
González-Díaz, Ángela
Meilhac, Olivier
Ureña, Juan
Amaya-Villar, Rosario
Castellano Orozco, Antonio Gonzalo
Varela Pérez, Lourdes María
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fisiología Médica y Biofísica
CTS591: Fisiopatología molecular del sistema cardiovascular
dc.subject.none.fl_str_mv Aneurysmal subarachnoid hemorrhage (aSAH)
Apolipoprotein A4 (APOA4)
High-density lipoproteins (HDL)
Serum amyloid A1 (SAA1)
Vascular cell adhesion protein-1 (VCAM-1)
topic Aneurysmal subarachnoid hemorrhage (aSAH)
Apolipoprotein A4 (APOA4)
High-density lipoproteins (HDL)
Serum amyloid A1 (SAA1)
Vascular cell adhesion protein-1 (VCAM-1)
description Aneurysmal subarachnoid hemorrhage (aSAH) is a devastating disease with high morbidity and mortality rates. Within 24 hours after aSAH, monocytes are recruited and enter the subarachnoid space, where they mature into macrophages, increasing the inflammatory response and contributing, along with other factors, to delayed neurological dysfunction and poor outcomes. High-density lipoproteins (HDL) are lipid-protein complexes that exert anti-inflammatory effects but under pathological conditions undergo structural alterations that have been associated with loss of functionality. Plasma HDL were isolated from patients with aSAH and analyzed for their anti-inflammatory activity and protein composition. HDLisolated from patients lost the ability to prevent VCAM-1 expression in endothelial cells (HUVEC) and subsequent adhesion of THP-1 monocytes to the endothelium. Proteomic analysis showed that HDL particles from patients had an altered composition compared to those of healthy subjects. We confirmed by western blot that low levels of apolipo protein A4 (APOA4) and high of serum amyloid A1 (SAA1) in HDL were associated with the lack of anti-inflammatory function observed in aSAH. Our results indicate that the study of HDL in the pathophysiology of aSAH is needed, and functional HDL supplementation could be considered a novel therapeutic approach to the treatment of the inflamma tory response after aSAH.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/167456
https://doi.org/10.1177/0271678x231184806
url https://hdl.handle.net/11441/167456
https://doi.org/10.1177/0271678x231184806
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of cerebral blood flow and metabolism, 43 (11), 1919-1930.
https://journals.sagepub.com/doi/10.1177/0271678X231184806
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sage
publisher.none.fl_str_mv Sage
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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