Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells

Plasma membrane tension regulates many key cellular processes. It is modulated by, and can modulate, membrane trafficking. However, the cellular pathway(s) involved in this interplay is poorly understood. Here we find that, among a number of endocytic processes operating simultaneously at the cell s...

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Autores: Thottacherry, Joseph Jose, Kosmalska, Anita Joanna, Kumar, Amit, Vishen, Amit S., Elosegui Artola, Alberto, Pradhan, Susav, Sharma, Sumit, Singh, Parvinder P., Guadamillas, Marta C., Chaudhary, Natasha, Vishwakarma, Ram, Trepat Guixer, Xavier, Pozo, Miguel A. del, Parton, Robert G., Rao, Madan, Pullarkat, Pramod, Roca-Cusachs Soulere, Pere, Mayor, Satyajit
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/139400
Acceso en línea:https://hdl.handle.net/2445/139400
Access Level:acceso abierto
Palabra clave:Biologia molecular
Transport biològic
Membranes (Biologia)
Molecular biology
Biological transport
Membranes (Biology)
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spelling Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cellsThottacherry, Joseph JoseKosmalska, Anita JoannaKumar, AmitVishen, Amit S.Elosegui Artola, AlbertoPradhan, SusavSharma, SumitSingh, Parvinder P.Guadamillas, Marta C.Chaudhary, NatashaVishwakarma, RamTrepat Guixer, XavierPozo, Miguel A. delParton, Robert G.Rao, MadanPullarkat, PramodRoca-Cusachs Soulere, PereMayor, SatyajitBiologia molecularTransport biològicMembranes (Biologia)Molecular biologyBiological transportMembranes (Biology)Plasma membrane tension regulates many key cellular processes. It is modulated by, and can modulate, membrane trafficking. However, the cellular pathway(s) involved in this interplay is poorly understood. Here we find that, among a number of endocytic processes operating simultaneously at the cell surface, a dynamin independent pathway, the CLIC/GEEC (CG) pathway, is rapidly and specifically upregulated upon a sudden reduction of tension. Moreover, inhibition (activation) of the CG pathway results in lower (higher) membrane tension. However, alteration in membrane tension does not directly modulate CG endocytosis. This requires vinculin, a mechano-transducer recruited to focal adhesion in adherent cells. Vinculin acts by controlling the levels of a key regulator of the CG pathway, GBF1, at the plasma membrane. Thus, the CG pathway directly regulates membrane tension and is in turn controlled via a mechano-chemical feedback inhibition, potentially leading to homeostatic regulation of membrane tension in adherent cells.Nature Publishing Group2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/139400Articles publicats en revistes (Biomedicina)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1038/s41467-018-06738-5Nature Communications, 2018, vol. 9, p. 4217https://doi.org/10.1038/s41467-018-06738-5info:eu-repo/grantAgreement/EC/H2020/731957info:eu-repo/grantAgreement/EC/FP7/616480cc-by (c) Thottacherry, Joseph J. et al., 2018http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1394002026-05-27T06:46:51Z
dc.title.none.fl_str_mv Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells
title Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells
spellingShingle Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells
Thottacherry, Joseph Jose
Biologia molecular
Transport biològic
Membranes (Biologia)
Molecular biology
Biological transport
Membranes (Biology)
title_short Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells
title_full Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells
title_fullStr Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells
title_full_unstemmed Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells
title_sort Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells
dc.creator.none.fl_str_mv Thottacherry, Joseph Jose
Kosmalska, Anita Joanna
Kumar, Amit
Vishen, Amit S.
Elosegui Artola, Alberto
Pradhan, Susav
Sharma, Sumit
Singh, Parvinder P.
Guadamillas, Marta C.
Chaudhary, Natasha
Vishwakarma, Ram
Trepat Guixer, Xavier
Pozo, Miguel A. del
Parton, Robert G.
Rao, Madan
Pullarkat, Pramod
Roca-Cusachs Soulere, Pere
Mayor, Satyajit
author Thottacherry, Joseph Jose
author_facet Thottacherry, Joseph Jose
Kosmalska, Anita Joanna
Kumar, Amit
Vishen, Amit S.
Elosegui Artola, Alberto
Pradhan, Susav
Sharma, Sumit
Singh, Parvinder P.
Guadamillas, Marta C.
Chaudhary, Natasha
Vishwakarma, Ram
Trepat Guixer, Xavier
Pozo, Miguel A. del
Parton, Robert G.
Rao, Madan
Pullarkat, Pramod
Roca-Cusachs Soulere, Pere
Mayor, Satyajit
author_role author
author2 Kosmalska, Anita Joanna
Kumar, Amit
Vishen, Amit S.
Elosegui Artola, Alberto
Pradhan, Susav
Sharma, Sumit
Singh, Parvinder P.
Guadamillas, Marta C.
Chaudhary, Natasha
Vishwakarma, Ram
Trepat Guixer, Xavier
Pozo, Miguel A. del
Parton, Robert G.
Rao, Madan
Pullarkat, Pramod
Roca-Cusachs Soulere, Pere
Mayor, Satyajit
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biologia molecular
Transport biològic
Membranes (Biologia)
Molecular biology
Biological transport
Membranes (Biology)
topic Biologia molecular
Transport biològic
Membranes (Biologia)
Molecular biology
Biological transport
Membranes (Biology)
description Plasma membrane tension regulates many key cellular processes. It is modulated by, and can modulate, membrane trafficking. However, the cellular pathway(s) involved in this interplay is poorly understood. Here we find that, among a number of endocytic processes operating simultaneously at the cell surface, a dynamin independent pathway, the CLIC/GEEC (CG) pathway, is rapidly and specifically upregulated upon a sudden reduction of tension. Moreover, inhibition (activation) of the CG pathway results in lower (higher) membrane tension. However, alteration in membrane tension does not directly modulate CG endocytosis. This requires vinculin, a mechano-transducer recruited to focal adhesion in adherent cells. Vinculin acts by controlling the levels of a key regulator of the CG pathway, GBF1, at the plasma membrane. Thus, the CG pathway directly regulates membrane tension and is in turn controlled via a mechano-chemical feedback inhibition, potentially leading to homeostatic regulation of membrane tension in adherent cells.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/139400
url https://hdl.handle.net/2445/139400
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1038/s41467-018-06738-5
Nature Communications, 2018, vol. 9, p. 4217
https://doi.org/10.1038/s41467-018-06738-5
info:eu-repo/grantAgreement/EC/H2020/731957
info:eu-repo/grantAgreement/EC/FP7/616480
dc.rights.none.fl_str_mv cc-by (c) Thottacherry, Joseph J. et al., 2018
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Thottacherry, Joseph J. et al., 2018
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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