Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells

Plasma membrane tension regulates many key cellular processes. It is modulated by, and can modulate, membrane trafficking. However, the cellular pathway(s) involved in this interplay is poorly understood. Here we find that, among a number of endocytic processes operating simultaneously at the cell s...

Descripción completa

Detalles Bibliográficos
Autores: Thottacherry, Joseph Jose, Kosmalska, Anita Joanna, Kumar, Amit, Vishen, Amit Singh, Elosegui-Artola, Alberto, Pradhan, Susav, Sharma, Sumit, Singh, Parvinder P., Guadamillas, Marta C., Chaudhary, Natasha, Vishwakarma, Ram, Trepat, Xavier, del Pozo, Miguel Angel, Parton, Robert G., Rao, Madan, Pullarkat, Pramod, Roca-Cusachs, Pere, Mayor, Satyajit
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/6529
Acceso en línea:http://hdl.handle.net/20.500.12105/6529
Access Level:acceso abierto
Palabra clave:CLATHRIN-MEDIATED ENDOCYTOSIS
FOCAL ADHESION DYNAMICS
TETHER FORMATION
MECHANOTRANSDUCTION
VINCULIN
ACTIVATION
FORCE
ENDOSOMES
INTEGRIN
PATHWAY
Descripción
Sumario:Plasma membrane tension regulates many key cellular processes. It is modulated by, and can modulate, membrane trafficking. However, the cellular pathway(s) involved in this interplay is poorly understood. Here we find that, among a number of endocytic processes operating simultaneously at the cell surface, a dynamin independent pathway, the CLIC/GEEC (CG) pathway, is rapidly and specifically upregulated upon a sudden reduction of tension. Moreover, inhibition (activation) of the CG pathway results in lower (higher) membrane tension. However, alteration in membrane tension does not directly modulate CG endocytosis. This requires vinculin, a mechano-transducer recruited to focal adhesion in adherent cells. Vinculin acts by controlling the levels of a key regulator of the CG pathway, GBF1, at the plasma membrane. Thus, the CG pathway directly regulates membrane tension and is in turn controlled via a mechano-chemical feedback inhibition, potentially leading to homeostatic regulation of membrane tension in adherent cells.

Similares en LA Referencia