Analysis of intracellular chitin deposition [Dataset]
All images show salivary glands. (A, C, E-L, N, O) Show single confocal sections and (B, D, M) show projections of several sections. (A-A’) The concomitant expression of reb and GFP-kkv leads to luminal chitin deposition (blue arrow in orthogonal section in A’). (B, C) Coexpression of expΔMH2 and GF...
| Autores: | , , , |
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| Tipo de recurso: | conjunto de datos |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/337675 |
| Acceso en línea: | http://hdl.handle.net/10261/337675 |
| Access Level: | acceso abierto |
| Palabra clave: | Highly abundant polymer Div >< p Chitin structural diversity Chitin accumulates intracellularly kkv subcellular localisation Fully elucidated yet Ectodermal tissues requires Apical extracellular matrices Elucidated yet Extracellular space Apical domain Chitin synthase Chitin deposition Chitin biosynthesis Topological distribution Rebuf reveals Proteins expansión Principal component Previously showed Multiple applications Molecular mechanisms Molecular análisis Mh2 domain Less punctae kkv complexes Excellent biomaterial Dynamic interplay Drosophila </ Conserved proteins Conserved nα Concomitant activities Although kkv |
| Sumario: | All images show salivary glands. (A, C, E-L, N, O) Show single confocal sections and (B, D, M) show projections of several sections. (A-A’) The concomitant expression of reb and GFP-kkv leads to luminal chitin deposition (blue arrow in orthogonal section in A’). (B, C) Coexpression of expΔMH2 and GFP-kkv produces intracellular chitin punctae, some of which partially colocalise with GFP-Kkv vesicles (yellow arrowhead) while others do not (red arrowhead). GFP-Kkv vesicles without chitin are also observed (green arrowhead). Note the accumulation of chitin in the apical domain (white arrow in C) that is not deposited extracellularly in the lumen (blue arrow in orthogonal section in C’). (D) In Rab5DN background, intracellular chitin punctae are still present (white arrowheads). (E-L) Analysis of the nature of GFP-Kkv vesicles and chitin punctae using markers Golgin245 (E-F), Hrs 27–4 (G-H), Arl8 (I-J), and Rab11 (K-L); arrowheads indicate colocalisation between Kkv and each specific marker. (M, N) All GFP-Kkv vesicles colocalise with the membrane marker CD4-mIFP (white arrowheads), and few of them also with chitin (orange arrowheads); single chitin punctae do not colocalise with CD4-mIFP (red arrowheads). (O-O”) Frames from live imaging movie show that partially colocalising GFP-Kkv and chitin punctae (yellow arrow) can separate from each other; however, many GFP-Kkv (green arrow) and chitin puncta (red arrow) do not colocalise. Scale bars A-D, M: 10 μm; E-L, N-N”‘: 1 μm; O-O”: 5 μm. |
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