Advanced Methods for Natural Products Discovery: Bioactivity Screening, Dereplication, Metabolomics Profiling, Genomic Sequencing, Databases and Informatic Tools, and Structure Elucidation

Natural Products (NP) are essential for the discovery of novel drugs and products for numerous biotechnological applications. The NP discovery process is expensive and time-consuming, having as major hurdles dereplication (early identification of known compounds) and structure elucidation, particula...

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Detalles Bibliográficos
Autores: Gaudêncio, Susana P., Bayram, Engin, Bilela, Lada Lukic, Cueto, Mercedes, Díaz-Marrero, Ana Raquel, Haznedaroglu, Berat Z., Jimenez, Carlos, Mandalakis, Manolis, Pereira, Florbela, Reyes, Fernando, Tasdemir, Deniz
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::3ed9b331448ff27ac42d1918a9005b89
Acceso en línea:http://hdl.handle.net/10261/309327
Access Level:acceso abierto
Palabra clave:Blue biotechnology
Natural products
high-throughput screening (HTS)
mode of action (MoA)
molecular networking
dereplication
natural products databases
Global Natural Product Social Molecular Networking (GNPS)
informatic chemometrics
high-throughput genome sequencing (HTGS)
computer assisted structure elucidation (CASE)
relative and absolute configuration determination in structure elucidation
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Descripción
Sumario:Natural Products (NP) are essential for the discovery of novel drugs and products for numerous biotechnological applications. The NP discovery process is expensive and time-consuming, having as major hurdles dereplication (early identification of known compounds) and structure elucidation, particularly the determination of the absolute configuration of metabolites with stereogenic centers. This review comprehensively focuses on recent technological and instrumental advances, highlighting the development of methods that alleviate these obstacles, paving the way for accelerating NP discovery towards biotechnological applications. Herein, we emphasize the most innovative high-throughput tools and methods for advancing bioactivity screening, NP chemical analysis, dereplication, metabolite profiling, metabolomics, genome sequencing and/or genomics approaches, databases, bioinformatics, chemoinformatics, and three-dimensional NP structure elucidation.