Respiration in Ndufs4−/− macrophages [Dataset]
(A) Activities of MRC complex (I–IV) were assayed spectrophotometrically, and the results were normalized to citrate synthase (CS) activity in mitochondria isolated from parental (Par) and Ndufs4−/− RAW 264.7 cells. (B) Left panel, a representative experiment showing OCR in RAW 264.7 sublines before...
| Autores: | , , , , , , , , |
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| Tipo de recurso: | conjunto de datos |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/352307 |
| Acceso en línea: | http://hdl.handle.net/10261/352307 |
| Access Level: | acceso abierto |
| Palabra clave: | Leigh syndrome patients Inflammatory cytokine profile Increasing evidence demonstrate Div >< p Mitochondrial respiration alterations Macrophage effector functions Effector functions Maximal respiration Vitro </ Supernumerary subunit Shift towards Phagocytose gram Ndufs4 </ Mutational hotspot Modulatory role Lower levels Lipopolysaccharide challenge Improved ability Critical role Atp production |
| Sumario: | (A) Activities of MRC complex (I–IV) were assayed spectrophotometrically, and the results were normalized to citrate synthase (CS) activity in mitochondria isolated from parental (Par) and Ndufs4−/− RAW 264.7 cells. (B) Left panel, a representative experiment showing OCR in RAW 264.7 sublines before and after the sequential addition of oligomycin (2.6 μM), FCCP (1 μM), and a combination of rotenone (Rot) and antimycin A (AA) (1 μM). Right panel, basal respiration, maximal respiration, and ATP production. Ns, not significant; *, P <0.05; **, P <0.01; ***, P<0.005; ****, P<0.001. Each point represents a biological replicate. Data are shown as the mean ± SEM. |
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