Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes

Cefepime is an antibiotic with a broad spectrum of antimicrobial activity. However, this antibiotic has several side effects and a high degradation rate. For this reason, the preparation and characterization of new liposomes that are able to encapsulate this antibiotic seem to be an important resear...

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Autores: Moyá Morán, María Luisa, López López, Manuel, Lebrón Romero, José Antonio, Ostos Marcos, Francisco José, Camacho Sánchez, Vanesa, Merino Bohórquez, Vicente, López-Cornejo, María del Pilar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/82954
Acceso en línea:https://hdl.handle.net/11441/82954
https://doi.org/10.3390/pharmaceutics11020069
Access Level:acceso abierto
Palabra clave:bactericidal activity
cefepime
encapsulation
Liposome
surfactant
zeta potentia
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spelling Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic LiposomesMoyá Morán, María LuisaLópez López, ManuelLebrón Romero, José AntonioOstos Marcos, Francisco JoséCamacho Sánchez, VanesaMerino Bohórquez, VicenteLópez-Cornejo, María del Pilarbactericidal activitycefepimeencapsulationLiposomesurfactantzeta potentiaCefepime is an antibiotic with a broad spectrum of antimicrobial activity. However, this antibiotic has several side effects and a high degradation rate. For this reason, the preparation and characterization of new liposomes that are able to encapsulate this antibiotic seem to be an important research line in the pharmaceutical industry. Anionic and cationic liposomes were prepared and characterized. All cationic structures contained the same cationic surfactant, N,N,N-triethyl-N-(12-naphthoxydodecyl)ammonium. Results showed a better encapsulation-efficiency percentage (EE%) of cefepime in liposomes with phosphatidylcholine and cholesterol than with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE). The presence of cholesterol and the quantity of egg-yolk phospholipid in the liposome increased the encapsulation percentage. The bactericidal activity against Escherichia coli of cefepime loaded into liposomes with phosphatidylcholine was measured. The inhibitory zone in an agar plate for free cefepime was similar to that obtained for loaded cefepime. The growth-rate constant of E. coli culture was also measured in working conditions. The liposome without any antibiotic exerted no influence in such a rate constant. All obtained results suggest that PC:CH:12NBr liposomes are biocompatible nanocarriers of cefepime that can be used in bacterial infections against Escherichia coli with high inhibitory activity.MDPIQuímica FísicaMicrobiología2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/82954https://doi.org/10.3390/pharmaceutics11020069reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésPharmaceutics, 11 (2), 1-12.https://doi.org/10.3390/pharmaceutics11020069info:eu-repo/semantics/openAccessoai:idus.us.es:11441/829542026-06-17T12:51:07Z
dc.title.none.fl_str_mv Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes
title Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes
spellingShingle Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes
Moyá Morán, María Luisa
bactericidal activity
cefepime
encapsulation
Liposome
surfactant
zeta potentia
title_short Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes
title_full Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes
title_fullStr Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes
title_full_unstemmed Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes
title_sort Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes
dc.creator.none.fl_str_mv Moyá Morán, María Luisa
López López, Manuel
Lebrón Romero, José Antonio
Ostos Marcos, Francisco José
Camacho Sánchez, Vanesa
Merino Bohórquez, Vicente
López-Cornejo, María del Pilar
author Moyá Morán, María Luisa
author_facet Moyá Morán, María Luisa
López López, Manuel
Lebrón Romero, José Antonio
Ostos Marcos, Francisco José
Camacho Sánchez, Vanesa
Merino Bohórquez, Vicente
López-Cornejo, María del Pilar
author_role author
author2 López López, Manuel
Lebrón Romero, José Antonio
Ostos Marcos, Francisco José
Camacho Sánchez, Vanesa
Merino Bohórquez, Vicente
López-Cornejo, María del Pilar
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Química Física
Microbiología
dc.subject.none.fl_str_mv bactericidal activity
cefepime
encapsulation
Liposome
surfactant
zeta potentia
topic bactericidal activity
cefepime
encapsulation
Liposome
surfactant
zeta potentia
description Cefepime is an antibiotic with a broad spectrum of antimicrobial activity. However, this antibiotic has several side effects and a high degradation rate. For this reason, the preparation and characterization of new liposomes that are able to encapsulate this antibiotic seem to be an important research line in the pharmaceutical industry. Anionic and cationic liposomes were prepared and characterized. All cationic structures contained the same cationic surfactant, N,N,N-triethyl-N-(12-naphthoxydodecyl)ammonium. Results showed a better encapsulation-efficiency percentage (EE%) of cefepime in liposomes with phosphatidylcholine and cholesterol than with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE). The presence of cholesterol and the quantity of egg-yolk phospholipid in the liposome increased the encapsulation percentage. The bactericidal activity against Escherichia coli of cefepime loaded into liposomes with phosphatidylcholine was measured. The inhibitory zone in an agar plate for free cefepime was similar to that obtained for loaded cefepime. The growth-rate constant of E. coli culture was also measured in working conditions. The liposome without any antibiotic exerted no influence in such a rate constant. All obtained results suggest that PC:CH:12NBr liposomes are biocompatible nanocarriers of cefepime that can be used in bacterial infections against Escherichia coli with high inhibitory activity.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/82954
https://doi.org/10.3390/pharmaceutics11020069
url https://hdl.handle.net/11441/82954
https://doi.org/10.3390/pharmaceutics11020069
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Pharmaceutics, 11 (2), 1-12.
https://doi.org/10.3390/pharmaceutics11020069
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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