Evaluation of parameters which influence voluntary ingestion of supplements in rats

This article belongs to the Special Issue Implementing the 3Rs in Laboratory Animal Research—From Theory to Practice.

Detalles Bibliográficos
Autores: Ruvira, Santiago, Rodríguez-Rodríguez, Pilar, Cañas, Silvia, Ramiro-Cortijo, David, Aguilera-Gutiérrez, Yolanda, Muñoz-Valverde, David, Arribas, Silvia M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/337996
Acceso en línea:http://hdl.handle.net/10261/337996
Access Level:acceso abierto
Palabra clave:Food supplement
Refinement
Rats
Sex
Gelatin
Habituation
Voluntary ingestion
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dc.title.none.fl_str_mv Evaluation of parameters which influence voluntary ingestion of supplements in rats
title Evaluation of parameters which influence voluntary ingestion of supplements in rats
spellingShingle Evaluation of parameters which influence voluntary ingestion of supplements in rats
Ruvira, Santiago
Food supplement
Refinement
Rats
Sex
Gelatin
Habituation
Voluntary ingestion
title_short Evaluation of parameters which influence voluntary ingestion of supplements in rats
title_full Evaluation of parameters which influence voluntary ingestion of supplements in rats
title_fullStr Evaluation of parameters which influence voluntary ingestion of supplements in rats
title_full_unstemmed Evaluation of parameters which influence voluntary ingestion of supplements in rats
title_sort Evaluation of parameters which influence voluntary ingestion of supplements in rats
dc.creator.none.fl_str_mv Ruvira, Santiago
Rodríguez-Rodríguez, Pilar
Cañas, Silvia
Ramiro-Cortijo, David
Aguilera-Gutiérrez, Yolanda
Muñoz-Valverde, David
Arribas, Silvia M.
author Ruvira, Santiago
author_facet Ruvira, Santiago
Rodríguez-Rodríguez, Pilar
Cañas, Silvia
Ramiro-Cortijo, David
Aguilera-Gutiérrez, Yolanda
Muñoz-Valverde, David
Arribas, Silvia M.
author_role author
author2 Rodríguez-Rodríguez, Pilar
Cañas, Silvia
Ramiro-Cortijo, David
Aguilera-Gutiérrez, Yolanda
Muñoz-Valverde, David
Arribas, Silvia M.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Food supplement
Refinement
Rats
Sex
Gelatin
Habituation
Voluntary ingestion
topic Food supplement
Refinement
Rats
Sex
Gelatin
Habituation
Voluntary ingestion
description This article belongs to the Special Issue Implementing the 3Rs in Laboratory Animal Research—From Theory to Practice.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/337996
url http://hdl.handle.net/10261/337996
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-097504-B-I00
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/ani13111827
https://doi.org/10.3390/ani13111827
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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repository.mail.fl_str_mv
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spelling Evaluation of parameters which influence voluntary ingestion of supplements in ratsRuvira, SantiagoRodríguez-Rodríguez, PilarCañas, SilviaRamiro-Cortijo, DavidAguilera-Gutiérrez, YolandaMuñoz-Valverde, DavidArribas, Silvia M.Food supplementRefinementRatsSexGelatinHabituationVoluntary ingestionThis article belongs to the Special Issue Implementing the 3Rs in Laboratory Animal Research—From Theory to Practice.Drug safety and efficacy studies frequently use oral gavage, but repetitive usage may cause problems. Administration through voluntary ingestion represents an opportunity for refinement. We aimed to develop a protocol for voluntary ingestion of gelatin-based supplements in rats, assessing the influence of age, sex, fasting (4 h), and additives (vanilla, VF; sucralose, S), and to test it in lactating dams. Three-week-old and 5-month-old Sprague-Dawley rats were placed individually in an empty cage containing a gelatin cube and trained daily (5 days/week), recording the day the whole cube was consumed (latency). Rats trained prior to gestation were offered a gelatin containing 250 mg/kg cocoa shell extract (CSE) during lactation. Rats that did not eat the cube after 8 training days were considered non-habituated, with a proportion similar in young males (7.1%), young females (11.1%), and adult females (10.3%), but significantly higher in adult males (39.3%). Excluding non-habituated rats, latency was 2–3 days, without differences between young and adult rats (p = 0.657) or between males and females (p = 0.189). VF or VF + S in the gelatin did not modify latency, while fasting significantly reduced it in females (p = 0.007) but not in males (p = 0.501). During lactation, trained females ate the CSE-gelatin within 1–5 min without litter problems. Conclusions: Acceptance of a gelatin-based supplement is negatively influenced by male sex, facilitated by fasting, and not modified by additives. Training is remembered after 2 months and does not interfere with lactation. Gelatin-based voluntary ingestion is suitable to administer drugs that need to pass through the digestive system, ensuring adequate dosage, and is important to detect non-habituated rats prior to the study. The current protocol may be implemented by training the rats in their own cage.[Simple Summary] Preclinical studies evaluating the safety and efficacy of new drugs require experimental animals, which is important to ensure both adequate dosage and animal welfare. Administration through voluntary ingestion can improve animal wellbeing. We aimed to develop a protocol using small gelatin cubes to train rats, assessing the influence of age, sex, fasting, flavors (vanilla), and sweeteners (sucralose) to accept the new food. We tested the usefulness of the protocol to supplement rats during lactation. We demonstrated that most animals were easily trained to accept the gelatin cube in 2–3 days. However, some rats refused the new food even after 8 days. The proportion of rats that did not train was higher in adult males compared to young males, adults, and young females, suggesting the influence of sex. Four-hour food deprivation reduced the time for acceptance only in females, but flavoring or sweeteners in the gelatin did not modify it. Rats trained prior to gestation remembered training 2 months later and ate a gelatin containing a supplement daily during lactation for 1–5 min, without problems with the pups. We conclude that gelatin-based supplementation can be used for drug studies in rats, ensuring adequate dosage and wellbeing, which is important for the detection of non-trained rats.This research was funded by Ministerio de Ciencia Innovación y Universidades (Spain), grant number RTI2018-097504-B-I00 and The APC was funded by D.R.-C.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/337996reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-097504-B-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/ani13111827https://doi.org/10.3390/ani13111827Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3379962026-05-22T06:33:51Z
score 15,81155

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