5-ht5B Receptor mRNA in the Raphe Nuclei: Coexpression with Serotonin Transporter

We used double-label in situ hybridization to examine the cellular localization of 5-ht5B receptor mRNA in relation to serotonin transporter mRNA in the rat dorsal raphe (DR) and central superior nucleus (CS, median raphe nucleus). 5-ht5B receptor mRNA hybridization signal was often found on seroton...

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Autores: Serrats, Jordi, Raurich, Andreu, Vilaró, Maria Teresa, Mengod Los Arcos, Guadalupe, Cortés, Roser
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2003
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/112549
Acceso en línea:http://hdl.handle.net/10261/112549
Access Level:acceso abierto
Palabra clave:Median raphe nucleus
Dorsal raphe
In situ hybridization
Autoreceptors
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spelling 5-ht5B Receptor mRNA in the Raphe Nuclei: Coexpression with Serotonin TransporterSerrats, JordiRaurich, AndreuVilaró, Maria TeresaMengod Los Arcos, GuadalupeCortés, RoserMedian raphe nucleusDorsal rapheIn situ hybridizationAutoreceptorsWe used double-label in situ hybridization to examine the cellular localization of 5-ht5B receptor mRNA in relation to serotonin transporter mRNA in the rat dorsal raphe (DR) and central superior nucleus (CS, median raphe nucleus). 5-ht5B receptor mRNA hybridization signal was often found on serotonin transporter mRNA-positive neuron profiles. The degree of cellular colocalization of these mRNAs notably varied among the different regions of the raphe nuclei. In the DR, cell bodies showing 5-ht5B receptor mRNA expression were abundant in the medial portions of the nucleus, all of them being also labeled for serotonin transporter mRNA. In contrast, in the ventrolateral regions (lateral wings) of the DR, we observed serotonin transporter mRNA-positive cells, but they were devoid of 5-ht5B receptor mRNA signal. In the CS, the level of coexpression of 5-ht5B receptor mRNA with serotonin transporter mRNA was high in the intermediate portions of the nucleus; however, we were unable to detect specific 5-ht 5B receptor mRNA hybridization signal in its caudal extent. Our results support the presence of 5-ht5B receptor in serotonergic neurons in the DR and CS, suggesting an autoreceptor role for this receptor subtype. © 2003 Wiley-Liss, Inc.Contract grant sponsor: >Ministerio de Ciencia y Tecnología> (MCYT); Contract grant numbers: SAF97-0117, SAF2000-0212; Contract grant sponsor: DURSI - >Generalitat de Catalunya> to the Department of Neurochemistry of IIBB-CSIC (IDIBAPS) as a >Grup de Recerca Consolidat> (5092-CSIC-01); Contract grant number: 2001-SGR-00355; Contract grant sponsors: >Institut d'Investigacions Biomèdiques August Pi i Synyer> (IDIBAPS) (fellowship >Beca de Formació de Personal Investigador> to JS), CIRIT (>Generalitt de Catalunya>) (fellowship to AR), the Ministerio de Ciencia y Tecnología (>Ramón y Cajal> contract to MTV)Peer ReviewedJohn Wiley & Sons2015201520032015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/112549reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1002/syn.10287info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1125492026-05-22T06:33:51Z
dc.title.none.fl_str_mv 5-ht5B Receptor mRNA in the Raphe Nuclei: Coexpression with Serotonin Transporter
title 5-ht5B Receptor mRNA in the Raphe Nuclei: Coexpression with Serotonin Transporter
spellingShingle 5-ht5B Receptor mRNA in the Raphe Nuclei: Coexpression with Serotonin Transporter
Serrats, Jordi
Median raphe nucleus
Dorsal raphe
In situ hybridization
Autoreceptors
title_short 5-ht5B Receptor mRNA in the Raphe Nuclei: Coexpression with Serotonin Transporter
title_full 5-ht5B Receptor mRNA in the Raphe Nuclei: Coexpression with Serotonin Transporter
title_fullStr 5-ht5B Receptor mRNA in the Raphe Nuclei: Coexpression with Serotonin Transporter
title_full_unstemmed 5-ht5B Receptor mRNA in the Raphe Nuclei: Coexpression with Serotonin Transporter
title_sort 5-ht5B Receptor mRNA in the Raphe Nuclei: Coexpression with Serotonin Transporter
dc.creator.none.fl_str_mv Serrats, Jordi
Raurich, Andreu
Vilaró, Maria Teresa
Mengod Los Arcos, Guadalupe
Cortés, Roser
author Serrats, Jordi
author_facet Serrats, Jordi
Raurich, Andreu
Vilaró, Maria Teresa
Mengod Los Arcos, Guadalupe
Cortés, Roser
author_role author
author2 Raurich, Andreu
Vilaró, Maria Teresa
Mengod Los Arcos, Guadalupe
Cortés, Roser
author2_role author
author
author
author
dc.subject.none.fl_str_mv Median raphe nucleus
Dorsal raphe
In situ hybridization
Autoreceptors
topic Median raphe nucleus
Dorsal raphe
In situ hybridization
Autoreceptors
description We used double-label in situ hybridization to examine the cellular localization of 5-ht5B receptor mRNA in relation to serotonin transporter mRNA in the rat dorsal raphe (DR) and central superior nucleus (CS, median raphe nucleus). 5-ht5B receptor mRNA hybridization signal was often found on serotonin transporter mRNA-positive neuron profiles. The degree of cellular colocalization of these mRNAs notably varied among the different regions of the raphe nuclei. In the DR, cell bodies showing 5-ht5B receptor mRNA expression were abundant in the medial portions of the nucleus, all of them being also labeled for serotonin transporter mRNA. In contrast, in the ventrolateral regions (lateral wings) of the DR, we observed serotonin transporter mRNA-positive cells, but they were devoid of 5-ht5B receptor mRNA signal. In the CS, the level of coexpression of 5-ht5B receptor mRNA with serotonin transporter mRNA was high in the intermediate portions of the nucleus; however, we were unable to detect specific 5-ht 5B receptor mRNA hybridization signal in its caudal extent. Our results support the presence of 5-ht5B receptor in serotonergic neurons in the DR and CS, suggesting an autoreceptor role for this receptor subtype. © 2003 Wiley-Liss, Inc.
publishDate 2003
dc.date.none.fl_str_mv 2003
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/112549
url http://hdl.handle.net/10261/112549
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1002/syn.10287
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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