5-ht5B Receptor mRNA in the Raphe Nuclei: Coexpression with Serotonin Transporter
We used double-label in situ hybridization to examine the cellular localization of 5-ht5B receptor mRNA in relation to serotonin transporter mRNA in the rat dorsal raphe (DR) and central superior nucleus (CS, median raphe nucleus). 5-ht5B receptor mRNA hybridization signal was often found on seroton...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2003 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/112549 |
| Acceso en línea: | http://hdl.handle.net/10261/112549 |
| Access Level: | acceso abierto |
| Palabra clave: | Median raphe nucleus Dorsal raphe In situ hybridization Autoreceptors |
| Sumario: | We used double-label in situ hybridization to examine the cellular localization of 5-ht5B receptor mRNA in relation to serotonin transporter mRNA in the rat dorsal raphe (DR) and central superior nucleus (CS, median raphe nucleus). 5-ht5B receptor mRNA hybridization signal was often found on serotonin transporter mRNA-positive neuron profiles. The degree of cellular colocalization of these mRNAs notably varied among the different regions of the raphe nuclei. In the DR, cell bodies showing 5-ht5B receptor mRNA expression were abundant in the medial portions of the nucleus, all of them being also labeled for serotonin transporter mRNA. In contrast, in the ventrolateral regions (lateral wings) of the DR, we observed serotonin transporter mRNA-positive cells, but they were devoid of 5-ht5B receptor mRNA signal. In the CS, the level of coexpression of 5-ht5B receptor mRNA with serotonin transporter mRNA was high in the intermediate portions of the nucleus; however, we were unable to detect specific 5-ht 5B receptor mRNA hybridization signal in its caudal extent. Our results support the presence of 5-ht5B receptor in serotonergic neurons in the DR and CS, suggesting an autoreceptor role for this receptor subtype. © 2003 Wiley-Liss, Inc. |
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