Boosting pro-vitamin A content and bioaccessibility in leaves by combining engineered biosynthesis and storage pathways with high-light treatments

[EN] Biofortification of green leafy vegetables with pro-vitamin A carotenoids, such as beta-carotene, has remained challenging to date. Here, we combined two strategies to achieve this goal. One of them involves producing beta-carotene in the cytosol of leaf cells to avoid the negative impacts on p...

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Autores: Morelli, Luca, Reig-Lopez, Diego, Di, Xueni, Llorente, Briardo, Pérez-Colao, Pablo, RODRIGUEZ-CONCEPCION, Manuel|||0000-0002-1280-2305
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/209357
Acceso en línea:https://riunet.upv.es/handle/10251/209357
Access Level:acceso abierto
Palabra clave:Beta-carotene
Bioaccessibility
Biofortification
Carotenoids
Lettuce
Plastoglobules
Vitamin A
02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
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network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Boosting pro-vitamin A content and bioaccessibility in leaves by combining engineered biosynthesis and storage pathways with high-light treatments
title Boosting pro-vitamin A content and bioaccessibility in leaves by combining engineered biosynthesis and storage pathways with high-light treatments
spellingShingle Boosting pro-vitamin A content and bioaccessibility in leaves by combining engineered biosynthesis and storage pathways with high-light treatments
Morelli, Luca
Beta-carotene
Bioaccessibility
Biofortification
Carotenoids
Lettuce
Plastoglobules
Vitamin A
02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
title_short Boosting pro-vitamin A content and bioaccessibility in leaves by combining engineered biosynthesis and storage pathways with high-light treatments
title_full Boosting pro-vitamin A content and bioaccessibility in leaves by combining engineered biosynthesis and storage pathways with high-light treatments
title_fullStr Boosting pro-vitamin A content and bioaccessibility in leaves by combining engineered biosynthesis and storage pathways with high-light treatments
title_full_unstemmed Boosting pro-vitamin A content and bioaccessibility in leaves by combining engineered biosynthesis and storage pathways with high-light treatments
title_sort Boosting pro-vitamin A content and bioaccessibility in leaves by combining engineered biosynthesis and storage pathways with high-light treatments
dc.creator.none.fl_str_mv Morelli, Luca
Reig-Lopez, Diego
Di, Xueni
Llorente, Briardo
Pérez-Colao, Pablo
RODRIGUEZ-CONCEPCION, Manuel|||0000-0002-1280-2305
author Morelli, Luca
author_facet Morelli, Luca
Reig-Lopez, Diego
Di, Xueni
Llorente, Briardo
Pérez-Colao, Pablo
RODRIGUEZ-CONCEPCION, Manuel|||0000-0002-1280-2305
author_role author
author2 Reig-Lopez, Diego
Di, Xueni
Llorente, Briardo
Pérez-Colao, Pablo
RODRIGUEZ-CONCEPCION, Manuel|||0000-0002-1280-2305
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
European Commission
Generalitat Valenciana
Agencia Estatal de Investigación
Gordon and Betty Moore Foundation
Ministerio de Ciencia e Innovación
Fundació Bancària Caixa d'Estalvis i Pensions de Barcelona
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Beta-carotene
Bioaccessibility
Biofortification
Carotenoids
Lettuce
Plastoglobules
Vitamin A
02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
topic Beta-carotene
Bioaccessibility
Biofortification
Carotenoids
Lettuce
Plastoglobules
Vitamin A
02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
description [EN] Biofortification of green leafy vegetables with pro-vitamin A carotenoids, such as beta-carotene, has remained challenging to date. Here, we combined two strategies to achieve this goal. One of them involves producing beta-carotene in the cytosol of leaf cells to avoid the negative impacts on photosynthesis derived from changing the balance of carotenoids and chlorophylls in chloroplasts. The second approach involves the conversion of chloroplasts into non-photosynthetic, carotenoid-overaccumulating chromoplasts in leaves agroinfiltrated or infected with constructs encoding the bacterial phytoene synthase crtB, leaving other non-engineered leaves of the plant to sustain normal growth. A combination of these two strategies, referred to as strategy C (for cytosolic production) and strategy P (for plastid conversion mediated by crtB), resulted in a 5-fold increase in the amount of beta-carotene in Nicotiana benthamiana leaves. Following several attempts to further improve beta-carotene leaf contents by metabolic engineering, hormone treatments and genetic screenings, it was found that promoting the proliferation of plastoglobules with increased light-intensity treatments not only improved beta-carotene accumulation but it also resulted in a much higher bioaccessibility. The combination of strategies C and P together with a more intense light treatment increased the levels of accessible beta-carotene 30-fold compared to controls. We further demonstrated that stimulating plastoglobule proliferation with strategy P, but also with a higher-light treatment alone, also improved beta-carotene contents and bioaccessibility in edible lettuce (Lactuca sativa) leaves.; Biofortification of leaves and other green tissues with pro-vitamin A carotenoids such as beta-carotene still remains challenging mostly due to the negative impact that altering carotenoid contents may have in photosynthesis. Here, we combined extraplastidial production and artificial chromoplast differentiation together with chemical (hormones) and physical (high-light) treatments to boost the beta-carotene contents of leaves while improving bioaccessibility, using Nicotiana benthamiana as the test system and lettuce (Lactuca sativa) as the crop model. image
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-09-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/209357
url https://riunet.upv.es/handle/10251/209357
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PCI2021-121941 USE OF TOMATO LINES TOLERANT TO PROXIMITY SHADE TO INCREASE YIELD AND QUALITY IN INTERCROPPING AGROSYSTEMS
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-115810GB-I00 MECANISMOS MOLECULARES DE LA BIOGENESIS DE CROMOPLASTOS
Generalitat Valenciana https://doi.org/10.13039/501100003359 PROMETEO%2F2021%2F056
Generalitat Valenciana https://doi.org/10.13039/501100003359 CIACIF%2F2021%2F278
Fundació Bancària Caixa d'Estalvis i Pensions de Barcelona Fundació Bancària Caixa d'Estalvis i Pensions de Barcelona LCF%2FBQ%2FIN18%2F11660004
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 RED2022-134577-T
Generalitat Valenciana https://doi.org/10.13039/501100003359 AGROALNEXT%2F2022%2F067
GBMF GBMF GBMF9319
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Boosting pro-vitamin A content and bioaccessibility in leaves by combining engineered biosynthesis and storage pathways with high-light treatmentsMorelli, LucaReig-Lopez, DiegoDi, XueniLlorente, BriardoPérez-Colao, PabloRODRIGUEZ-CONCEPCION, Manuel|||0000-0002-1280-2305Beta-caroteneBioaccessibilityBiofortificationCarotenoidsLettucePlastoglobulesVitamin A02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades[EN] Biofortification of green leafy vegetables with pro-vitamin A carotenoids, such as beta-carotene, has remained challenging to date. Here, we combined two strategies to achieve this goal. One of them involves producing beta-carotene in the cytosol of leaf cells to avoid the negative impacts on photosynthesis derived from changing the balance of carotenoids and chlorophylls in chloroplasts. The second approach involves the conversion of chloroplasts into non-photosynthetic, carotenoid-overaccumulating chromoplasts in leaves agroinfiltrated or infected with constructs encoding the bacterial phytoene synthase crtB, leaving other non-engineered leaves of the plant to sustain normal growth. A combination of these two strategies, referred to as strategy C (for cytosolic production) and strategy P (for plastid conversion mediated by crtB), resulted in a 5-fold increase in the amount of beta-carotene in Nicotiana benthamiana leaves. Following several attempts to further improve beta-carotene leaf contents by metabolic engineering, hormone treatments and genetic screenings, it was found that promoting the proliferation of plastoglobules with increased light-intensity treatments not only improved beta-carotene accumulation but it also resulted in a much higher bioaccessibility. The combination of strategies C and P together with a more intense light treatment increased the levels of accessible beta-carotene 30-fold compared to controls. We further demonstrated that stimulating plastoglobule proliferation with strategy P, but also with a higher-light treatment alone, also improved beta-carotene contents and bioaccessibility in edible lettuce (Lactuca sativa) leaves.; Biofortification of leaves and other green tissues with pro-vitamin A carotenoids such as beta-carotene still remains challenging mostly due to the negative impact that altering carotenoid contents may have in photosynthesis. Here, we combined extraplastidial production and artificial chromoplast differentiation together with chemical (hormones) and physical (high-light) treatments to boost the beta-carotene contents of leaves while improving bioaccessibility, using Nicotiana benthamiana as the test system and lettuce (Lactuca sativa) as the crop model. imageWe thank Trine B. Andersen and Jose A. Daros for materials, Jose Luis Micol for the ron1-2 mutant, Felix Kessler for the vte1 mutant, Alberto Coronado-Martin for help with protoplast isolation, and M. Rosa Rodriguez, Jose Perez-Beser and the staff at the IBMCP Metabolomics Platform for technical support. We also thank the Microscopy Section at the University of Valencia SCSIE and Maria T. Minguez for her specialized support with electron microscopy. This work was funded by grants from Spanish MCIN/AEI/10.13039/501100011033 and European NextGeneration EU/PRTR and PRIMA programs to MR-C (PID2020-115810GB-I00 and UToPIQ-PCI2021-121941). MR-C is also supported by Generalitat Valenciana (PROMETEU/2021/056 and AGROALNEXT/2022/067) and the MCIN/AEI-funded Spanish Carotenoid Network, CaRed (RED2022-134577-T). BL acknowledges the support of the Gordon and Betty Moore Foundation (GBMF9319, grant DOI: ), the ARC Centre of Excellence for Synthetic Biology, Twist Bioscience, and the Allen Foundation. LM and PP-C received predoctoral fellowships from La Caixa Foundation (INPhINIT program LCF/BQ/IN18/11660004) and Generalitat Valenciana (CIACIF/2021/278), respectively.Blackwell PublishingInstituto Universitario Mixto de Biología Molecular y Celular de PlantasEuropean CommissionGeneralitat ValencianaAgencia Estatal de InvestigaciónGordon and Betty Moore FoundationMinisterio de Ciencia e InnovaciónFundació Bancària Caixa d'Estalvis i Pensions de BarcelonaRepositorio Institucional de la Universitat Politècnica de València Riunet20242024-09-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/209357reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PCI2021-121941 USE OF TOMATO LINES TOLERANT TO PROXIMITY SHADE TO INCREASE YIELD AND QUALITY IN INTERCROPPING AGROSYSTEMSAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-115810GB-I00 MECANISMOS MOLECULARES DE LA BIOGENESIS DE CROMOPLASTOSGeneralitat Valenciana https://doi.org/10.13039/501100003359 PROMETEO%2F2021%2F056Generalitat Valenciana https://doi.org/10.13039/501100003359 CIACIF%2F2021%2F278Fundació Bancària Caixa d'Estalvis i Pensions de Barcelona Fundació Bancària Caixa d'Estalvis i Pensions de Barcelona LCF%2FBQ%2FIN18%2F11660004Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 RED2022-134577-TGeneralitat Valenciana https://doi.org/10.13039/501100003359 AGROALNEXT%2F2022%2F067GBMF GBMF GBMF9319open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2093572026-06-13T07:49:27Z
score 15,812429

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