Laser Reduced Graphene Oxide Electrode for Pathogenic Escherichia coli Detection

Graphene-based materials are of interest in electrochemical biosensing due to their unique properties, such as high surface areas, unique electrochemical properties, and biocompatibility. However, the scalable production of graphene electrodes remains a challenge; it is typically slow, expensive, an...

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Autores: Zhao, Lei|||0000-0003-0291-2655, Rosati, Giulio|||0000-0002-0227-4561, Piper, Andrew|||0000-0002-1208-8009, De Carvalho Castro Silva, Cecilia|||0000-0003-3933-1838, Hu, Liming|||0000-0002-8666-9287, Yang, Qiuyue|||0000-0002-7693-6679, Della Pelle, Flavio|||0000-0002-8877-7580, Álvarez Diduk, Ruslan|||0000-0002-9876-1574, Merkoçi, Arben|||0000-0003-2486-8085
Tipo de documento: artigo
Data de publicação:2023
País:España
Recursos:Universitat Autònoma de Barcelona
Repositório:Dipòsit Digital de Documents de la UAB
Idioma:inglês
OAI Identifier:oai:ddd.uab.cat:272461
Acesso em linha:https://ddd.uab.cat/record/272461
https://dx.doi.org/urn:doi:10.1021/acsami.2c20859
Access Level:Acceso aberto
Palavra-chave:Graphene electrodes
Fabrication
Biosensing
Nanomaterials
Bacteria detection
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spelling Laser Reduced Graphene Oxide Electrode for Pathogenic Escherichia coli DetectionZhao, Lei|||0000-0003-0291-2655Rosati, Giulio|||0000-0002-0227-4561Piper, Andrew|||0000-0002-1208-8009De Carvalho Castro Silva, Cecilia|||0000-0003-3933-1838Hu, Liming|||0000-0002-8666-9287Yang, Qiuyue|||0000-0002-7693-6679Della Pelle, Flavio|||0000-0002-8877-7580Álvarez Diduk, Ruslan|||0000-0002-9876-1574Merkoçi, Arben|||0000-0003-2486-8085Graphene electrodesFabricationBiosensingNanomaterialsBacteria detectionGraphene-based materials are of interest in electrochemical biosensing due to their unique properties, such as high surface areas, unique electrochemical properties, and biocompatibility. However, the scalable production of graphene electrodes remains a challenge; it is typically slow, expensive, and inefficient. Herein, we reported a simple, fast, and maskless method for large-scale, low-cost reduced graphene oxide electrode fabrication; using direct writing (laser scribing and inkjet printing) coupled with a stamp-transferring method. In this process, graphene oxide is simultaneously reduced and patterned with a laser, before being press-stamped onto polyester sheets. The transferred electrodes were characterized by SEM, XPS, Raman, and electrochemical methods. The biosensing utility of the electrodes was demonstrated by developing an electrochemical test for Escherichia coli. These biosensors exhibited a wide dynamic range (917-2.1 × 10 7 CFU/mL) of low limits of detection (283 CFU/mL) using just 5 μL of sample. The test was also verified in spiked artificial urine, and the sensor was integrated into a portable wireless system driven and measured by a smartphone. This work demonstrates the potential to use these biosensors for real-world, point-of-care applications. Hypothetically, the devices are suitable for the detection of other pathogenic bacteria. 22023-01-0120232023-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/272461https://dx.doi.org/urn:doi:10.1021/acsami.2c20859reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 SEV-2017-0706European Commission https://doi.org/10.13039/501100000780 825694open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2724612026-06-06T12:50:31Z
dc.title.none.fl_str_mv Laser Reduced Graphene Oxide Electrode for Pathogenic Escherichia coli Detection
title Laser Reduced Graphene Oxide Electrode for Pathogenic Escherichia coli Detection
spellingShingle Laser Reduced Graphene Oxide Electrode for Pathogenic Escherichia coli Detection
Zhao, Lei|||0000-0003-0291-2655
Graphene electrodes
Fabrication
Biosensing
Nanomaterials
Bacteria detection
title_short Laser Reduced Graphene Oxide Electrode for Pathogenic Escherichia coli Detection
title_full Laser Reduced Graphene Oxide Electrode for Pathogenic Escherichia coli Detection
title_fullStr Laser Reduced Graphene Oxide Electrode for Pathogenic Escherichia coli Detection
title_full_unstemmed Laser Reduced Graphene Oxide Electrode for Pathogenic Escherichia coli Detection
title_sort Laser Reduced Graphene Oxide Electrode for Pathogenic Escherichia coli Detection
dc.creator.none.fl_str_mv Zhao, Lei|||0000-0003-0291-2655
Rosati, Giulio|||0000-0002-0227-4561
Piper, Andrew|||0000-0002-1208-8009
De Carvalho Castro Silva, Cecilia|||0000-0003-3933-1838
Hu, Liming|||0000-0002-8666-9287
Yang, Qiuyue|||0000-0002-7693-6679
Della Pelle, Flavio|||0000-0002-8877-7580
Álvarez Diduk, Ruslan|||0000-0002-9876-1574
Merkoçi, Arben|||0000-0003-2486-8085
author Zhao, Lei|||0000-0003-0291-2655
author_facet Zhao, Lei|||0000-0003-0291-2655
Rosati, Giulio|||0000-0002-0227-4561
Piper, Andrew|||0000-0002-1208-8009
De Carvalho Castro Silva, Cecilia|||0000-0003-3933-1838
Hu, Liming|||0000-0002-8666-9287
Yang, Qiuyue|||0000-0002-7693-6679
Della Pelle, Flavio|||0000-0002-8877-7580
Álvarez Diduk, Ruslan|||0000-0002-9876-1574
Merkoçi, Arben|||0000-0003-2486-8085
author_role author
author2 Rosati, Giulio|||0000-0002-0227-4561
Piper, Andrew|||0000-0002-1208-8009
De Carvalho Castro Silva, Cecilia|||0000-0003-3933-1838
Hu, Liming|||0000-0002-8666-9287
Yang, Qiuyue|||0000-0002-7693-6679
Della Pelle, Flavio|||0000-0002-8877-7580
Álvarez Diduk, Ruslan|||0000-0002-9876-1574
Merkoçi, Arben|||0000-0003-2486-8085
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Graphene electrodes
Fabrication
Biosensing
Nanomaterials
Bacteria detection
topic Graphene electrodes
Fabrication
Biosensing
Nanomaterials
Bacteria detection
description Graphene-based materials are of interest in electrochemical biosensing due to their unique properties, such as high surface areas, unique electrochemical properties, and biocompatibility. However, the scalable production of graphene electrodes remains a challenge; it is typically slow, expensive, and inefficient. Herein, we reported a simple, fast, and maskless method for large-scale, low-cost reduced graphene oxide electrode fabrication; using direct writing (laser scribing and inkjet printing) coupled with a stamp-transferring method. In this process, graphene oxide is simultaneously reduced and patterned with a laser, before being press-stamped onto polyester sheets. The transferred electrodes were characterized by SEM, XPS, Raman, and electrochemical methods. The biosensing utility of the electrodes was demonstrated by developing an electrochemical test for Escherichia coli. These biosensors exhibited a wide dynamic range (917-2.1 × 10 7 CFU/mL) of low limits of detection (283 CFU/mL) using just 5 μL of sample. The test was also verified in spiked artificial urine, and the sensor was integrated into a portable wireless system driven and measured by a smartphone. This work demonstrates the potential to use these biosensors for real-world, point-of-care applications. Hypothetically, the devices are suitable for the detection of other pathogenic bacteria.
publishDate 2023
dc.date.none.fl_str_mv 2
2023-01-01
2023
2023-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/272461
https://dx.doi.org/urn:doi:10.1021/acsami.2c20859
url https://ddd.uab.cat/record/272461
https://dx.doi.org/urn:doi:10.1021/acsami.2c20859
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 SEV-2017-0706
European Commission https://doi.org/10.13039/501100000780 825694
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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