Differential DNA methylation patterns between high and low responders to a weight loss intervention in overweight or obese adolescents: the EVASYON study

In recent years, epigenetic markers emerged as a new tool to understand the influence of lifestyle factors on obesity phenotypes. Adolescence is considered an important epigenetic window over a human's lifetime. The objective of this work was to explore baseline changes in DNA methylation that...

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Autores: Moleres, A. (Adriana)|||/items/f83af6e2-75d6-4841-87d9-82e40a3a2a70, Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb, Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c, Marcos, A. (Ascensión)|||/items/3819856a-a083-497b-9603-0f673a978740, Campoy, C. (Cristina)|||/items/029daec1-bfbf-407b-bde4-116059a92af6, Garagorri, J.M. (Jesús Maria)|||/items/e82b532e-c986-4ef7-b102-b0a56108dc55, Gomez-Martinez, S. (Sonia)|||/items/94f4bc4a-0671-48a2-b4cf-d3fab009cbad, Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131, Azcona-San-Julián, M.C. (María Cristina)|||/items/89d7bb2f-b633-4413-bc4d-cddf6026e848, Marti-del-Moral, A. (Amelia)|||/items/c62ba83d-ba7d-43aa-944c-3b57a06af670
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/29096
Acceso en línea:https://hdl.handle.net/10171/29096
Access Level:acceso abierto
Palabra clave:Epigenetics
Weight loss intervention
Biomarkers
Dieting response
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spelling Differential DNA methylation patterns between high and low responders to a weight loss intervention in overweight or obese adolescents: the EVASYON studyMoleres, A. (Adriana)|||/items/f83af6e2-75d6-4841-87d9-82e40a3a2a70Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724ebCampión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817cMarcos, A. (Ascensión)|||/items/3819856a-a083-497b-9603-0f673a978740Campoy, C. (Cristina)|||/items/029daec1-bfbf-407b-bde4-116059a92af6Garagorri, J.M. (Jesús Maria)|||/items/e82b532e-c986-4ef7-b102-b0a56108dc55Gomez-Martinez, S. (Sonia)|||/items/94f4bc4a-0671-48a2-b4cf-d3fab009cbadMartinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131Azcona-San-Julián, M.C. (María Cristina)|||/items/89d7bb2f-b633-4413-bc4d-cddf6026e848Marti-del-Moral, A. (Amelia)|||/items/c62ba83d-ba7d-43aa-944c-3b57a06af670EpigeneticsWeight loss interventionBiomarkersDieting responseIn recent years, epigenetic markers emerged as a new tool to understand the influence of lifestyle factors on obesity phenotypes. Adolescence is considered an important epigenetic window over a human's lifetime. The objective of this work was to explore baseline changes in DNA methylation that could be associated with a better weight loss response after a multidisciplinary intervention program in Spanish obese or overweight adolescents. Overweight or obese adolescents (n=107) undergoing 10 wk of a multidisciplinary intervention for weight loss were assigned as high or low responders to the treatment. A methylation microarray was performed to search for baseline epigenetic differences between the 2 groups (12 subjects/group), and MALDI-TOF mass spectrometry was used to validate (n=107) relevant CpG sites and surrounding regions. After validation, 5 regions located in or near AQP9, DUSP22, HIPK3, TNNT1, and TNNI3 genes showed differential methylation levels between high and low responders to the multidisciplinary weight loss intervention. Moreover, a calculated methylation score was significantly associated with changes in weight, BMI-SDS, and body fat mass loss after the treatment. In summary, we have identified 5 DNA regions that are differentially methylated depending on weight loss response. These methylation changes may help to better understand the weight loss response in obese adolescents.Federation of American Society of Experimental Biology (FASEB)Dadun. Depósito Académico Digital Universidad de Navarra20132013-04-2220132013-01-0120132013-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleimage/jpegimage/jpegimage/jpegimage/jpegapplication/pdfimage/jpegapplication/pdfhttps://hdl.handle.net/10171/29096reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/290962026-06-21T12:47:57Z
dc.title.none.fl_str_mv Differential DNA methylation patterns between high and low responders to a weight loss intervention in overweight or obese adolescents: the EVASYON study
title Differential DNA methylation patterns between high and low responders to a weight loss intervention in overweight or obese adolescents: the EVASYON study
spellingShingle Differential DNA methylation patterns between high and low responders to a weight loss intervention in overweight or obese adolescents: the EVASYON study
Moleres, A. (Adriana)|||/items/f83af6e2-75d6-4841-87d9-82e40a3a2a70
Epigenetics
Weight loss intervention
Biomarkers
Dieting response
title_short Differential DNA methylation patterns between high and low responders to a weight loss intervention in overweight or obese adolescents: the EVASYON study
title_full Differential DNA methylation patterns between high and low responders to a weight loss intervention in overweight or obese adolescents: the EVASYON study
title_fullStr Differential DNA methylation patterns between high and low responders to a weight loss intervention in overweight or obese adolescents: the EVASYON study
title_full_unstemmed Differential DNA methylation patterns between high and low responders to a weight loss intervention in overweight or obese adolescents: the EVASYON study
title_sort Differential DNA methylation patterns between high and low responders to a weight loss intervention in overweight or obese adolescents: the EVASYON study
dc.creator.none.fl_str_mv Moleres, A. (Adriana)|||/items/f83af6e2-75d6-4841-87d9-82e40a3a2a70
Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb
Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c
Marcos, A. (Ascensión)|||/items/3819856a-a083-497b-9603-0f673a978740
Campoy, C. (Cristina)|||/items/029daec1-bfbf-407b-bde4-116059a92af6
Garagorri, J.M. (Jesús Maria)|||/items/e82b532e-c986-4ef7-b102-b0a56108dc55
Gomez-Martinez, S. (Sonia)|||/items/94f4bc4a-0671-48a2-b4cf-d3fab009cbad
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
Azcona-San-Julián, M.C. (María Cristina)|||/items/89d7bb2f-b633-4413-bc4d-cddf6026e848
Marti-del-Moral, A. (Amelia)|||/items/c62ba83d-ba7d-43aa-944c-3b57a06af670
author Moleres, A. (Adriana)|||/items/f83af6e2-75d6-4841-87d9-82e40a3a2a70
author_facet Moleres, A. (Adriana)|||/items/f83af6e2-75d6-4841-87d9-82e40a3a2a70
Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb
Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c
Marcos, A. (Ascensión)|||/items/3819856a-a083-497b-9603-0f673a978740
Campoy, C. (Cristina)|||/items/029daec1-bfbf-407b-bde4-116059a92af6
Garagorri, J.M. (Jesús Maria)|||/items/e82b532e-c986-4ef7-b102-b0a56108dc55
Gomez-Martinez, S. (Sonia)|||/items/94f4bc4a-0671-48a2-b4cf-d3fab009cbad
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
Azcona-San-Julián, M.C. (María Cristina)|||/items/89d7bb2f-b633-4413-bc4d-cddf6026e848
Marti-del-Moral, A. (Amelia)|||/items/c62ba83d-ba7d-43aa-944c-3b57a06af670
author_role author
author2 Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb
Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c
Marcos, A. (Ascensión)|||/items/3819856a-a083-497b-9603-0f673a978740
Campoy, C. (Cristina)|||/items/029daec1-bfbf-407b-bde4-116059a92af6
Garagorri, J.M. (Jesús Maria)|||/items/e82b532e-c986-4ef7-b102-b0a56108dc55
Gomez-Martinez, S. (Sonia)|||/items/94f4bc4a-0671-48a2-b4cf-d3fab009cbad
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
Azcona-San-Julián, M.C. (María Cristina)|||/items/89d7bb2f-b633-4413-bc4d-cddf6026e848
Marti-del-Moral, A. (Amelia)|||/items/c62ba83d-ba7d-43aa-944c-3b57a06af670
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Epigenetics
Weight loss intervention
Biomarkers
Dieting response
topic Epigenetics
Weight loss intervention
Biomarkers
Dieting response
description In recent years, epigenetic markers emerged as a new tool to understand the influence of lifestyle factors on obesity phenotypes. Adolescence is considered an important epigenetic window over a human's lifetime. The objective of this work was to explore baseline changes in DNA methylation that could be associated with a better weight loss response after a multidisciplinary intervention program in Spanish obese or overweight adolescents. Overweight or obese adolescents (n=107) undergoing 10 wk of a multidisciplinary intervention for weight loss were assigned as high or low responders to the treatment. A methylation microarray was performed to search for baseline epigenetic differences between the 2 groups (12 subjects/group), and MALDI-TOF mass spectrometry was used to validate (n=107) relevant CpG sites and surrounding regions. After validation, 5 regions located in or near AQP9, DUSP22, HIPK3, TNNT1, and TNNI3 genes showed differential methylation levels between high and low responders to the multidisciplinary weight loss intervention. Moreover, a calculated methylation score was significantly associated with changes in weight, BMI-SDS, and body fat mass loss after the treatment. In summary, we have identified 5 DNA regions that are differentially methylated depending on weight loss response. These methylation changes may help to better understand the weight loss response in obese adolescents.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-04-22
2013
2013-01-01
2013
2013-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/29096
url https://hdl.handle.net/10171/29096
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv image/jpeg
image/jpeg
image/jpeg
image/jpeg
application/pdf
image/jpeg
application/pdf
dc.publisher.none.fl_str_mv Federation of American Society of Experimental Biology (FASEB)
publisher.none.fl_str_mv Federation of American Society of Experimental Biology (FASEB)
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
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