Graph based study of allergen cross-reactivity of plant lipid transfer proteins (LTPs) using microarray in a multicenter study.

The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay....

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Detalles Bibliográficos
Autores: Palacín, Arantxa, Gómez-Casado, Cristina, Rivas, Luis A, Aguirre, Jacobo, Tordesillas, Leticia, Bartra, Joan, Carrillo, Teresa, Cuesta-Herranz, Javier, Frutos, Consolación de, García Alvarez-Eire, Genoveva, Fernández, Francisco J, Gamboa, Pedro, Muñoz, Rosa, Sánchez-Monge, Rosa, Sirvent, Sofía, Torres, María J, Varela-Losada, Susana, Rodríguez, Rosalía, Parro, Victor, Blanca, Miguel, Salcedo, Gabriel, Díaz-Perales, Araceli
Tipo de recurso: artículo
Fecha de publicación:2012
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/17044
Acceso en línea:http://hdl.handle.net/20.500.12105/17044
Access Level:acceso abierto
Palabra clave:Reacciones Cruzadas
Estudios multicéntricos como asunto
Hipersensibilidad
Análisis de micromatrices
Inmunoanálisis
España
Cross Reactions
Carrier Proteins
Plant Proteins
Antigens, Plant
Multicenter Studies as Topic
Hypersensitivity
Microarray Analysis
Immunoassay
Fruit
Plants
Spain
Pollen
Descripción
Sumario:The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay. This enabled us to include a wide number of proteins and a large number of patients, and to study sensitization profiles among members of the LTP family. Fourteen LTPs from the most frequent plant food-induced allergies in the geographical area studied were printed into a microarray specifically designed for this research. 212 patients with fruit allergy and 117 food-tolerant pollen allergic subjects were recruited from seven regions of Spain with different pollen profiles, and their sera were tested with allergen microarray. This approach has proven itself to be a good tool to study cross-reactivity between members of LTP family, and could become a useful strategy to analyze other families of allergens.

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